Edc4 (NM_001301100) Mouse Untagged Clone

CAT#: MC229581

Edc4 (untagged) - Mouse enhancer of mRNA decapping 4 (Edc4), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Edc4
• Synonyms: BC022641
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229581 representing NM_001301100
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCTGCGCGAGCATCGACATTGAGGACGCCACGCAGCATCTGCGGGACATACTCAAACTGGACC
  GGCCCGCCGGAGGTTCCAATGCCGAAAGCCAAAGGCCATCCAGTGCCTACAATGGAGACCTCAATGGGCT
  CCTGGTCCCCGACCCGCTCAGTTCAGGTGATGGTAACTCAACGAACAAACCTGGTATACGGACCATGCCA
  CCCATTAACCTGCAGGAAAAGCAGGTCATTTGCCTCTCTGGAGATGACAGCTCCACCTGCATTGGGATTT
  TAGCCAAGGAAGTGGAAATTGTGGCCAGCAGTGATTCTAGCATTTCCAGCAAGGCTCGGGGGAGCAACAA
  GGTGAAAATCCAGCCTGTCGCCAAGTATGACTGGGAGCAGAAATACTACTATGGCAACCTGATTGCAGTG
  TCTAACTCCTTCTTGGCATACGCCATTAGGGCTGCCAACAATGGCTCAGCAATGGTCCGTGTGATCAGTG
  TCAGCACTTCGGAGCGGACCCTGCTCAAAGGTTTCACAGGCAGTGTGGCTGATCTTGCTTTTGCACACCT
  CAACTCTCCACAGCTCGCCTGCCTGGATGAGGCTGGCAATCTTTTTGTGTGGCGCTTGGCTCTTGTTAAG
  GGTAAAATTCAAGAAGAGATTTTAGTCCATATCCGGCAGCCAGAGGGTACAGCGCTGAACCACTTCCGAA
  GGATCATCTGGTGCCCTTTCATCCCCGAGGAGAGTGAGGACTGTTGTGAGGAGAGCAGCCCGACAGTGGC
  CCTGCTGCATGAGGATCGGGCTGAGGTGTGGGACTTGGATATGCTTCGCTCCAGCCACAACACCTGGCCT
  GTGGATGTCAGCCAAATCAAGCAAGGCTTTATTGTGGTGAAAGGCCATAGCACGTGCCTAAGTGAAGGAG
  CCCTCTCCCCTGATGGGACTGTCCTGGCTACCGCAAGCCATGATGGCTTTGTCAAGTTCTGGCAGATCTA
  TATTGAAGGTCAGGATGAGCCAAGGTGTCTGCATGAATGGAAGCCTCATGATGGGAGGCCTCTTTCTTGC
  CTCCTGTTCTGTGATAACCATAAGAAACAGGATCCTGAGGTCCCGTTCTGGAGGTTCCTCATAACCGGCG
  CCGACCAGAATCGAGAGCTGAAGATGTGGTGCACAGTGTCCTGGACCTGCCTGCAAACCATTCGCTTCTC
  CCCAGATATCTTCAGCTCAGTGAGTGTGCCTCCCAGCCTCAAAGTTTGTTTGGACCTCTCAGCAGAGTAT
  TTGATTCTTAGTGATGTGCAACGAAAGGTCCTCTATGTGATGGAGCTGCTGCAGAACCAGGACGAGGGCC
  GCGCGTGCTTTAGTTCTATCTCTGAGTTCCTGCTCACCCACCCTGTGCTGAGCTTTGGCATCCAGGTTGT
  CAGTCGCTGCCGGCTACGGCACACTGAGGTCCTGCCTGCTGAGGAGGAGAATGACAGTCTGGGGACCGAG
  AGTTCCCATGGAGCTGGAGCCTTGGAATCCGCAGCTGGTGTGCTTATCAAGCTCTTTTGTGTGCACACTA
  AGGCACTACAAGATGTGCAGATCCGCTTCCAGCCACAGCTGAACCCTGATGTGGTGGCCCCACTCTCTAC
  CCACACTGCCCACGAGGACTTCACATTTGGAGAGTCTCGACCTGAACTAGGCTCCGAGGGGCTAGCTTCA
  GCTGCTCATGGTTCCCAGCCTGACCTGCGGCGCATTGTGGAACTGCCTGCTCCTGCAGACTTCCTCAGTC
  TGAGCAGTGAGACCAAGCCCAAGTTGATGACACCTGATGCTTTCATGACACCTACTGCCTCCCTGCAGCA
  GATCTCTGCATCCCCTAGTAGTAGCAGCAGCAGTAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGC
  AGCTCTCTAACAGCTGTGTCTGCTGTGAGCAGCTCCTCAGCCATGGACCCTTCCTTGCCCAGACCACCTG
  AGGAGCTGACCTTGAGCCCCAAGCTGCAGCTAGATGGCAGCCTGACGCTAAACAGCAGCAGCAGCAGCCT
  GCAGGCAAGCCCTCGAAGCCTCCTTCCGGGCCTGCTCCCAGGCCCAGCTGACAAGTTGATTTCCAAGGGA
  CCTGGTCAGGTATCCACTGCTGCCTCTGCACTGTCCTTGGATTTGCAGGAAGTGGAGCCATTAGGGCTAC
  CCCAGGCCTCCCCAAGTCGAACCCGTTCTCCTGATGTGATCTCCTCAGCATCCACTGCCCTGTCCCAGGA
  CATTCCTGAAATCGCATCTGAGGCCCTGTCCCGTGGCTTTGGCTCCTCTGTTCCTGAGGGCCTCATTGAG
  CCAGACAGCATGGCCTCAGCTGCCTCAGCACTACACCTACTCTCTCCTCGGCCCAGGCAAGGCCCTGAGC
  TTGGCTCTCAGCTGGGTCTGGATGGAGGCCCTGGAGATGGGGATAGGCATAGTACCCCTTCCCTGCTGGA
  AGCAGCCCTGACCCAGGAAGTTGCAACCCCTGACAGTCAAGTCTGGCCTACGGCACCTGACATTACTCGT
  GAGACCTGTAGCACACTGACAGAAAGCCCCAGGAATGGCCTCCAGGAAAAGCACAAAAGTCTGGCTTTCC
  ACAGGCCACCTTATCACTTGCTGCAGCAACGTGACAGTCAGGACACAAGTGCTGAGCAAAGTGACCATGA
  TGATGAGGTTGCCAGCCTTGCCTCTGCCTCAGGAGGTTTTGGCAGCAAAATTCCTACTCCACGGCTGCCT
  TCCAAGGATTGGAAGACGAAGGGATCCCCTCGGACGTCACCTAAGCTCAAGAGAAAAAGCAAGAAGGATG
  ATGGGGATTCAGCTGTGGGATCCCGGCTCACAGAGCACCAGGTGGCAGAGCCCCCTGAAGACTGGCCAGC
  ACTAATTTGGCAGCAGCAAAGAGAGCTGGCAGAGCTATGGCACAACCAAGAAGAACTGCTACAGCGTCTC
  TGTGCCCAGCTTGAAGGTCTTCAGAGTACTGTCACAGACCATGTAGAGCGTGCCCTGGAGACGCGGCATG
  AGCAGGAGCAACGGCGGCTGGAACGGGCACTGGCTGAGGGGCAGCAACGGGGTGGGCAGCTGCAGGAGCA
  GCTGACACAGCAGCTATCCCAGGCATTGTCTTCAGCTGTGGCTGGGCGGCTAGAGCGCAGCGTAAGGGAT
  GAAATCAAGAAAACGGTTCCTCCATGTGTCTCCAGAAGTCTGGAGCCTGTTGCAGGTCAACTAAGCAACT
  CAGTGGCTACCAAGCTTACAGCTGTGGAAGGCAGCATGAAAGAGAATATCTCTAAGCTACTCAAGTCCAA
  GAACTTAACAGATGCCATTGCCCGAGCAGCTGCAGACACGTTACAGGGACCAATGCAGGCTGCCTACCGG
  GAAGCCTTCCAGAGTGTGGTACTGCCAGCTTTTGAGAAGAGTTGCCAGGCTATGTTCCAGCAAATCAATG
  ACAGCTTTAGGCTGGGCACACAGGAATATTTGCAGCAGCTGGAGAGTCACATGAAGAGCCGGAAGGCCCG
  TGAACAAGAAGCTAGGGAGCCTGTGCTGGCCCAGCTTCGGGGCCTGGTCAGCACACTGCAGAGTGCCACC
  GAGCAGATGGCGGCCACTGTATCTAGCAGTGTTCGGGCCGAGGTGCAACACCAGCTGCATGTGGCTGTGG
  GCAGCTTGCAGGAGTCAATCTTAGCACAAGTACAGCGCATTGTCAAGGGTGAAGTGAGTGTGGCACTTAA
  GGAGCAGCAGGCCACCGTCACTTCCAGCATCATGCAGGCTATGCGTTCAGCTGCTGGCACACCTGTCCCC
  TCTGCCCACCTTGACTGCCAGGCCCAACAAGCCCATATCTTGCAGTTACTGCAGCAGGGCCACCTCAATC
  AGGCCTTCCAGCAGGCACTGACTGCTGCTGATCTCAATCTGGTGCTGTATGTGTGTGAAACTGTCGACCC
  AGCCCAGGTTTTTGGGCAGCCGCCCTGTCCACTATCCCAGCCTGTTCTCCTTTCCCTAATCCAGCAGCTA
  GCATCTGACCTTGGAACTAGAAGTGACCTCAAGCTCAGCTACTTGGAAGAAGCTGTGATGCACCTGGACC
  ACAGCGATCCCATCACTCGAGACCACATGGGCTCTGTCATGGCTCAGGTGCGCCAGAAGCTCTTCCAGTT
  CCTGCAGGCTGATCCACACAACTCACTTAGCAAAGCTGCCCGCCGCCTCAGCCTAATGTTACACGGCCTT
  GTGACCCCTAGCCTCCCTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301100
• Insert Size: 4221 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301100.1, NP_001288029.1
• RefSeq Size: 4803 bp
• RefSeq ORF: 4221 bp
• Locus ID: 234699
• UniProt ID: Q3UJB9
• Cytogenetics: 8 D3
• Gene Summary: The protein encoded by this gene is thought to promote mRNA decay, and is known to interact with several mRNA decapping proteins. In humans, decreased expression of this gene prevents the accumulation of mRNA decapping proteins to mRNA processing bodies (P-body). Alternative splicing results in multiple protein isoforms. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).