Ncoa2 (NM_001302702) Mouse Untagged Clone

CAT#: MC229574

Ncoa2 (untagged) - Mouse nuclear receptor coactivator 2 (Ncoa2), transcript variant c

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ncoa2
• Synonyms: bHLHe75; D1Ertd433e; GRIP-1; Grip1; KAT13C; NCoA-2; SRC-2; TIF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229574 representing NM_001302702
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGGATGGGAGAAAACACCTCTGACCCGTCCAGGGCAGAGACCAGAAAACGCAAGGAATGTCCCG
  ACCAGCTCGGACCCAGCCCCAAAAGGAGCACTGAGAAACGGAACCGCGAGCAGGAGAATAAGTACATAGA
  GGAGCTGGCCGAGCTGATCTTCGCAAACTTTAATGATATTGACAACTTCAACTTCAAACCTGACAAATGT
  GCCATCCTAAAAGAAACTGTGAAGCAGATCCGCCAGATCAAAGAGCAAGAGAAAGCAGCAGCTGCCAACA
  TAGATGAAGTGCAGAAGTCAGATGTGTCGTCCACGGGGCAGGGTGTCATCGACAAGGATGCACTGGGGCC
  CATGATGCTTGAGGCCCTCGATGGGTTCTTCTTCGTTGTGAACCTGGAAGGCAGTGTGGTGTTCGTGTCA
  GAGAATGTGACACAGTATCTACGGTATAACCAAGAAGAGCTGATGAACAAGAGTGTCTACAGCATCCTGC
  ATGTCGGGGACCACACTGAATTTGTCAAGAACCTGCTGCCAAAGTCCATGGTGAATGGAGGATCCTGGTC
  TGGAGAACCTCCCAGGCGGAGCAGCCATACCTTCAACTGTCGCATGCTGGTGAAGCCTTTGCCAGATTCA
  GAAGAGGAAGGCCATGATAGCCAGGAAGCCCATCAGAAATACGAGGCGATGCAGTGCTTCGCTGTGTCTC
  AGCCCAAGTCCATCAAAGAGGAAGGCGAAGATTTGCAGTCCTGCTTGATTTGTGTGGCACGAAGAGTCCC
  CATGAAGGAAAGACCAACTCTTCCCTCATCAGAAAGCTTTACCACCCGCCAGGACCTCCAAGGCAAGATC
  ACTTCACTGGACACTAGCACCATGAGAGCCGCCATGAAGCCGGGCTGGGAAGATCTGGTAAGAAGATGCA
  TTCAGAAGTTCCACACACAGCATGAAGGGGAGTCTCTATCATATGCCAAGAGGCATCACCATGAAGTTCT
  GAGACAAGGGTTGGCGTTCAGTCAGATCTATCGTTTTTCTTTGTCTGATGGCACTCTCGTTGCTGCACAA
  ACCAAGAGCAAACTCATCCGTTCTCAGACTACTAATGAGCCTCAGCTTGTAATATCTTTACACATGCTTC
  ACAGAGAGCAGAATGTATGTGTAATGAATCCGGATCTGACTGGACAAGCGATGGGGAAGCCATTGAATCC
  AATTAGCTCTAGCAGCCCTGCCCACCAGGCCCTGTGCAGTGGGAACCCAGGTCAGGACATGACCCTCGGT
  AGCAATATAAATTTTCCCATGAATGGCCCAAAGGAACAAATGGGCATGCCTATGGGCAGGTTTGGTGGTT
  CTGGGGGCATGAACCATGTGTCAGGCATGCAAGCAACCACTCCTCAGGGTAGTAACTATGCACTCAAAAT
  GAACAGTCCCTCGCAAAGCAGCCCCGGCATGAACCCGGGGCAAGCCAGCTCCGTGCTCTCCCCAAGGCAG
  CGCATGAGCCCCGGCGTGGCTGGCAGTCCTCGCATCCCACCCAGTCAGTTTTCCCCTGCAGGAAGCTTGC
  ATTCCCCTGTGGGAGTTTGCAGCAGCACAGGAAATAGCCATAGTTATACCAACAGTTCCCTCAATGCACT
  GCAAGCCCTCAGCGAGGGCCATGGGGTCTCACTCGGGTCCTCGCTGGCTTCACCGGACCTAAAAATGGGC
  AATTTGCAAAACTCCCCAGTTAATATGAATCCTCCCCCACTCAGCAAGATGGGAAGCTTGGACTCCAAAG
  ACTGTTTTGGACTTTATGGGGAGCCCTCAGAAGGTACAACTGGACAAGCAGAGGCCAGCTGCCATCCTGA
  AGAACAAAAGGGGCCCAATGATTCCAGCATGCCCCAGGCGGCCAGCGGGGACAGGGCTGAGGGACACAGC
  CGGCTGCATGACAGCAAAGGGCAGACCAAACTCCTGCAGCTGCTGACCACCAAGTCCGACCAGATGGAGC
  CTTCACCCTTGCCCAGCTCCTTGTCGGACACAAACAAGGACTCAACAGGGAGCTTGCCTGGGCCTGGGTC
  CACGCATGGCACCTCGCTCAAGGAGAAGCATAAGATTTTGCACAGACTCTTACAGGACAGCAGTTCCCCT
  GTGGACTTGGCCAAGCTGACAGCAGAAGCCACAGGCAAAGAGCTGAGCCAGGAGTCCAGCAGCACAGCTC
  CTGGGTCGGAAGTGACTGTCAAACAGGAGCCAGCGAGCCCCAAGAAGAAAGAGAATGCACTACTGCGCTA
  TTTGCTCGACAAAGATGATACTAAAGATATTGGTTTACCGGAAATAACCCCCAAACTCGAGCGACTGGAC
  AGTAAGACAGATCCTGCCAGTAACACAAAGTTAATTGCTATGAAAACTGTGAAGGAGGAGGTGAGCTTTG
  AGCCCAGTGACCAGCCTGGCAGCGAGCTGGACAACTTGGAAGAGATTTTGGATGATTTGCAGAACAGTCA
  GTTACCACAGCTTTTCCCAGACACAAGGCCAGGAGCTCCTACTGGGTCAGTTGACAAGCAAGCCATCATC
  AATGACCTCATGCAACTCACAGCTGACAGCAGTCCCGTCCCACCTGCCGGAGCCCAGAAGGCAGCACTGC
  GCATGTCACAGAGCAGAATGATTGGCAGCAGCACTTCCCGGCCCAGCATGCCTTCTGGGGAATGGGCACC
  ACAGAGTCCAGCTGTGAGAGTCACTTGTGCTGCTACCACTGGTGCCATGAACCGACCAGTCCAAGGAGGC
  ATGATTCGGAACCCAACAGCCAGCATCCCCATGCGAGCCAACAGCCAGCCTGGCCAAAGACAGATGCTTC
  AGTCTCAGGTCATGAACATAGGCCCTTCTGAGTTAGAGATGAACATGGGAGGACCTCAGTATAATCAACA
  GCAGGCCCCTCCGAACCAAACTGCCCCGTGGCCTGAGAGCATCCTGCCTATAGACCAGGCATCGTTTGCC
  AGCCAGAACAGGCAGCCCTTCGGCAGCTCCCCTGATGACCTGCTGTGTCCACATCCTGCAGCAGAGTCGC
  CAAGCGATGAGGGCGCTCTTCTTGACCAGCTGTATCTGGCCTTGCGGAACTTCGATGGCCTTGAGGAGAT
  TGATAGAGCTCTGGGGATACCAGAACTGGTCAGCCAGAGCCAAGCTGTGGATGCAGAGCAGTTCTCAAGT
  CAGGAGTCCAGCATAATGCTGGAGCAGAAGCCCCCCGTTTTCCCACAGCAGTACGCATCTCAGGCACAAA
  TGGCCCAGGGTGGCTATAATCCCATGCAAGATCCAAACTTTCACACCATGGGACAGCGGCCAAATTACAC
  CACACTCCGTATGCAGCCACGGCCAGGCCTCAGGCCCACAGGCATTGTGCAGAACCAGCCAAACCAACTG
  AGACTTCAGCTTCAGCACCGCCTCCAAGCACAGCAGAATCGCCAGCCGCTTATGAATCAGATCAGCAGTG
  TTTCCAATGTGAACCTGACTCTGAGGCCTGGAGTGCCCACTCAGGCTCCTATTAATGCACAGATGCTGGC
  CCAGAGGCAGAGGGAAATCCTCAACCAACATCTTCGGCAGAGACAGATGCAGCAGCAGGTGCAGCAGCGG
  ACTTTGATGATGAGAGGACAGGGCTTGAATGTGACCCCAAGCATGGTGGCTCCCGCTGGCCTACCAGCAG
  CCATGAGCAATCCCCGGATCCCCCAGGCCAATGCCCAGCAGTTCCCATTTCCTCCGAACTACGGAATAAG
  TCAACAACCTGATCCTGGCTTTACTGGGGCTACAACTCCCCAGAGTCCTCTAATGTCTCCCCGGATGGCA
  CATACTCAGAGTCCCATGATGCAGCAGTCTCAAGCCAACCCAGCCTACCAGCCCACCTCAGACATGAATG
  GATGGGCACAGGGGAGCATGGGTGGAAACAGCATGTTCTCACAGCAGTCCCCACCACACTTTGGGCAACA
  AGCAAACACCAGCATGTATAGTAACAACATGAACATCAGTGTGTCGATGGCAACCAACACGGGTGGCTTG
  AGCAGCATGAACCAGATGACAGGCCAGATGAGCATGACCTCAGTGACCTCCGTGCCTACGTCAGGACTGC
  CCTCCATGGGTCCCGAGCAGGTCAATGACCCTGCTCTGAGGGGAGGCAACCTTTTCCCAAACCAACTGCC
  TGGAATGGACATGATCAAGCAGGAGGGAGATGCATCTCGGAAATACTGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302702
• Insert Size: 4182 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302702.1, NP_001289631.1
• RefSeq Size: 8180 bp
• RefSeq ORF: 4182 bp
• Locus ID: 17978
• Cytogenetics: 1 4.12 cM
• Gene Summary: Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as RORA coactivator to specifically modulate G6PC expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3. Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-ARNTL/BMAL1 heterodimer (PubMed:24529706).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (c) differs in the 5' UTR and lacks an in-frame exon compared to variant a. The encoded isoform (b) is shorter than isoform a.