Gprasp1 (NM_001005385) Mouse Untagged Clone

CAT#: MC229567

Gprasp1 (untagged) - Mouse G protein-coupled receptor associated sorting protein 1 (Gprasp1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gprasp1
• Synonyms: 2210415K24Rik; 3110031O14Rik; C87852; GASP; GASP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229567 representing NM_001005385
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAGGGCAGAGGTTGAGCCTGGTGCCCAGGCAAAGGCTGAAAATAAGCCTGGGGATGAGAATGCTA
  ATGCTGCTGAAGTAGAGCCTGAAGCCCCATTGGTGGTCAGACCCAAGGTTAGGACACAAATCATGACTGG
  AGCAAGGCCCAAAGTGAAGCCTAAGGGTACCCCTGGAGCAAGACCTAAAGGTGAAACCAGTACACCAGGT
  GGGGCATATGCCAAATGTAAACCTAAGGCAATCCCTATTGCTCGATCCAAGCATGATGCCCAAGTGTGGG
  CCCCAAATAAGTTTCGTGGCGAGTCCATGTCAAAGATGGGAAAGCAATGTCAGATCAGTGCTGCAGACCC
  TCCACTGCTCAGTAATGATTCTGGGATGGTTGCTCAAGCTAAGTGCCTGCCTGTAGATAGAGAACTTGCT
  AATATGGACACTGAGAGTATTCCCAAAAAGGCTAATTCTCCAGCTGGATTCCAGCCTTCCTATGGCTCAG
  AGGAGGGAACCAATATGGGGTCCTGGTACCGTGCCAGGCCTGTCCCCAAAGGAGAGGCCTATGAGAATTC
  CGATTTCAAATGGGCAGATAAACCCTCAGGAAGTCCCTCCTTTTGGAATAGAGATGAAGCCAGTACAAGA
  TTTCGTCCTAGGAAAAGCATGAAGGCCAATAACAGGTTCAGGCATATGGCCAAACAAGAGGCAAATACCA
  TGCCTAGGCACAAAAACAAACAAGAGTTCTATAATATTTCCAGTTCTGATTCTGAGGATGAGTCTGGTAA
  AACTCCCTGGTTCTGGCCCAAAGACAAGACCAAAGTCTGGTCTAAGCCCAAAGAAGAGCCCAATAGTAGG
  TCCTGGTTTAGGTCTAAGAAAGAAGTCCGTGTTGAATCCACTTCTGGGTCTGAATGTGAAAATCCTACAA
  AATCTTTGTTTTGGTCTGGAGAAGAGGCTAAATCTAGATCCAAACCCAGAGCCAGGAAAGGGGTCAATAT
  GAGGGCCAGGCAGCAGGCCAAGCGAGAAGCTTGCAGTGATGCCATGTCTGGAGCTATAGATACAAATAAG
  AAAGAGTCCTGGTTCTTGCCTGAAGAGAAAGCTAATGTTTTTTCAAAGTCTAAGACAAAGAAAGAGCCCA
  GAACCAGAGCAGTGCCAAAGGAAGAAGTCAAAACTAAGGCCAGAGCAAGTACCAAGCAAGAAGCCAGGCC
  AGAGGAGGAAGTCCTCGTTGGGGCTTGGTTCTGGGACACCCAGGAGTCCACTATGGCAGATAGGATAAGC
  ATCAAGACCACTTTTGTAGAGGAGGAGCCCATTGTTGGAGATTGGTTCTGGAGTGAAGAAGAAGCCAGTG
  TGGACTCTGAGACCTGTCATACATCCAGACCCAGGGCTAAGGAGGAGCAAGTTAGTAGTTTCTGTTTAGG
  GTCTGGAAAGAAGAGCAGTATGGAAAGTGGGCCAAAGGCTACTTCTAAATCTATGCCAGTAGCTAAGGAG
  GATGAGGTCGTTATTGGGTCCTGGTTCTGGGCAGATGATGAAGAGATCAACTTACAGGCTGATGACGAGT
  CTATTTTTGGATCTTGGTTCTGGGGCACTGGTGAGAACAGCCTTAGATCTGTTGGAGTCAATTGTGAGAA
  GATGCCGAAGGCTGGAGAAAAAGAAGTTACTGATTCCTGGTTCTGGGCTGGAGATGTCAATACAGAGGCT
  GAGGTTGAAGAACAGGCCAGGTCGGCATCTACAAAAGCAACAATCTTTGTGCCTTGGTTTTGGTCTGAAA
  AGCAGCCGAACATGGATTTAGGGTCTGAACCTTGCTCAGATATCATGGCAGGAGCTGAGGAGGAGCCCAT
  AATTGGGCCCTGGTTCTGGGCTAAAGTAGATAATAGTGTGGAGGCTGAAGTTAACAGTAAGTCTAGCCTT
  GAGGATGAGGAAGAACCCATTAGATCCCCTTGGTTTGGGGCCAGAGAACAAACCGATATGAAGTATGCAG
  CTGGTATCAGATACAAGCCTATGGCAGAAGCTGAGGATGCTAACAAAAAGTCTTGTGTCTGGGCAAAAGA
  ACCCTGTTTGTATCCTACCAATAGAGAATGCTTGAAATCTACTCTGGGAGAAAAAGAAGACACTGTTGAT
  CCATGGCTCTGGTCCAATAATTATCCAAGGACCAAGACCATCACAGGGTCCTGGCTATGGGCTGCAGAAG
  AGGGAAATATAGATGATGAGACTGGAGAAAAGATTAAACTACCAACTTTAGAGGACAATGCATTCAATTC
  TTGGTTCTGGAAAGAAAATGAAGAAAGCATTGTAGAGGCTCCCAAGAGAGAAGAATTCAGGCCAGAAGCG
  GAAGAGGAAGACATTATTGGTTCTTGGTTTTGGGCTGGGGATGAAGACAGGTTTGAGCCAGCTGCTAAGA
  TTAATGAAGAGAACAAGATAGCATCTGAAGATGAAGATACAGTTGGATCCTGGTTCTGGGGCAATGAAGA
  GGCCAGTCTAGAGGCAGTGAGAAGGGGTACTTTTGAGTCTGCTCCTGGGATTAAGGAAGAGAAAGTTACT
  GGGTCATGGTTCTGGACTGATAAGGCCAAAGTAGGGGCTGGGTCCCAGACTGTCGAAACTGGGTCAGAAA
  CTGAAGAGGAAGCAATTTTTGAGTCCTTGATCTGGGCTGCAAAAAAGGACAGTATTCAAGCAGGAGTAAA
  GCGTGTGTCCAAGCCAAAAGATGACGGTAACATAGCTGTTGGGTCCTGGCTCTGGTCTAGTGACAAGGCC
  ACAAAAGAAGCTAAAACTCTGATTGTCAGTGAGGCCAGTCCAGAAAATGGGAAAGAGTCAGTTGTTAAGT
  TTGGGTCAAGAGCAAAAGATGAGGTGATTAACAAGACTGGCAGTGGTGACAATTGTAAACATAGTACAGA
  AGCTGAAACCATAGTGGGAGCCTGGTTCTGGGAAGGAGATGAAGCTAGCTTTGAATCAAATCCTGTACCT
  GTATGCAAGGCCGTTTGTGAACCCGAGTCTTCAGCTGAGCACGAACCTGACCCTTCCCGCAGGCCTCAGA
  GCTGGGATGAGGTCACTGTTCAGTTTAAGGCTGGTCCGTGGGGAAAAGCTGGCTTCCCACCCATGAACCC
  CTTCAGATTCCCCAAAGAAGCGGCATCTCTGTTTGCAGAAATGTTTGGGGGAAAGCCTAAGCTTGTGGAA
  GTTGGTCCAGAAAGGGAGCCTGAACCCCAGTTCCCATTTCAGTATGATCCCTCTTACCGCTCAGTCCGAG
  AAATTCGCGAGCATCTTAAGGCCAGGGAAAGTGCACAGCCAGAGAATTGGTCTTGCAACTGCATCCAGTG
  TGAGCTTAGAATTGGTTCTGAGGAGTTTGAGGAGCTTCTTTTACTGATGGACAGAAATCGTGATCCTTTT
  ATCCATGAGATATCTAAGATTGCAATGGGCATGCGAGGTGCTTCTCAGTTTACCCGGGATTTCATTCGAA
  ACTCGGGCGTTGTCTCCCTGATTGAAGCCTTACTTAACTATCCGTCCTCCCGAGTGAGGACAAGGTTTTT
  GGAAAATATGGTCCGCATGGCTCCCCCTTATCCAGACCTGAACATGATTGAGACATACGTGTGTCAGATT
  TGCGAGGACACCTTTGACTATGACTTGGATTCCCCTGATCAGTTGTCCGGATTGACAATGATTACACACC
  TCACTGCTACTTCTGACTATCACAAGGTGGTTGTCAATTACTTGGCTGGGTTTTTCTACTTACTGAATTC
  AGGCAATACCAAAACAAGGTTTCATGTTCTGAAACTGCTACTGAATTTGTCTGAAAACCTTGTCATGACA
  AAACGCCTACTTGTTACTGATTCGGTGTCAGAATTTATGGACCTTATTAATAGGGAGGAGTCAGATGAAA
  ACATTCAAATTGTTCTTGCAATATTTGAGACTATCAGTAAACATATTCAAAAAGAGGCACTGTTTTCTGA
  TGATGATGATGATGATGAAGAAGAAGATGCAGTGAATCTTGAACCATTTATTTCTGCATTCCGTGAGGCT
  GAAAAAATTGCCAAGGAACTAAAACGCAAACCTGGCAATCAAAAAGCACCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001005385
• Insert Size: 4044 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005385.1, NP_001005385.1
• RefSeq Size: 5697 bp
• RefSeq ORF: 4044 bp
• Locus ID: 67298
• UniProt ID: Q5U4C1
• Cytogenetics: X F1
• Gene Summary: Modulates lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. Targets receptors for degradation in lysosomes via its interaction with BECN2.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created using sequences from two different strains, C57BL/6J and C57BL/6, because no single transcript from the same strain was available for the full length of the gene.