Dctn1 (NM_001198867) Mouse Untagged Clone

CAT#: MC229506

Dctn1 (untagged) - Mouse dynactin 1 (Dctn1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dctn1
• Synonyms: AL022633; DAP-150; DP-150; Glued; p150; p150-glued; p150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229506 representing NM_001198867
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTACGGAGGCAAGCGCCCGGCCCCTGCGGGTTGGCTCCCGCGTGGAGGTGATTGGGAAGGGCCACC
  GAGGCACTGTGGCCTATGTTGGAGCCACACTCTTTGCCACTGGCAAATGGGTGGGCGTGATTCTGGATGA
  AGCAAAAGGCAAAAATGATGGCACTGTCCAGGGAAGGAAGTATTTCACATGTGATGAAGGCCACGGCATC
  TTTGTACGCCAGTCCCAGATCCAAGTATTTGAAGATGGAGCAGATACTACTTCCCCAGAGACTCCTGATT
  CTTCTGCTTCAAAGGTCCTCAAGAGAGAGGGAGCCGATGCAGCTGCAAAGACCAGCAAACTGCCTACTCG
  CCCAGCCAGCACTGGGGTGGCTGGGCCCAGTAGCTCCCTTGGCCCCTCTGGCTCAGCGTCAGCCGGGGAA
  CTAAGCAGCAGTGAGCCCAGCACCCCAGCTCAGACTCCGCTGGCAGCACCCATCATCCCCACACCGGCCC
  TCACCTCTCCTGGAGCAGCACCCCCACTTCCATCTCCCTCTAAGGAAGAGGAAGGGCTGAGGGCTCAGGT
  ACGGGACCTGGAGGAGAAGCTGGAGACCCTGCGCCTAAAACGCTCAGAAGACAAAGCAAAGCTGAAAGAG
  CTGGAGAAGCACAAGATCCAGCTGGAGCAGGTGCAGGAATGGAAGAGCAAAATGCAGGAGCAGCAGGCAG
  ACCTGCAGCGGCGCCTCAAGGAGGCTCGGAAGGAAGCCAAGGAGGCGCTAGAGGCAAAGGAACGCTACAT
  GGAGGAGATGGCCGACACAGCCGACGCTATCGAGATGGCCACTCTGGACAAGGAGATGGCTGAAGAGCGC
  GCTGAGTCTCTGCAGCAAGAGGTGGAGGCACTGAAGGAACGGGTAGACGAGCTCACCACAGACCTGGAGA
  TTCTCAAGGCTGAAATCGAAGAGAAAGGCTCTGATGGGGCCGCATCAAGCTACCAGCTCAAGCAGCTGGA
  GGAGCAGAATGCCCGCCTGAAGGATGCCCTGGTGAGGATGCGAGACCTCTCTTCCTCAGAGAAGCAGGAG
  CACGTGAAGCTGCAGAAACTCATGGAAAAGAAAAACCAGGAGCTGGAGGTCGTGCGGCAGCAGCGCGAGC
  GTCTTCAGGAGGAGCTGAGCCAGGCTGAGAGCACCATCGATGAGCTCAAAGAGCAGGTGGACGCCGCTCT
  GGGAGCCGAGGAGATGGTGGAGATGCTGACCGACCGGAACCTGAATCTAGAGGAGAAAGTGCGGGAGTTA
  CGGGAGACTGTGGGGGACTTGGAAGCCATGAACGAGATGAACGATGAGCTGCAGGAGAACGCACGGGAGA
  CGGAGCTGGAACTCCGAGAGCAGCTGGACATGGCGGGCGCCCGAGTGAGGGAAGCGCAGAAGCGAGTGGA
  AGCCGCCCAGGAGACAGTCGCCGACTACCAGCAGACCATCAAGAAGTACCGCCAGTTGACTGCCCACCTA
  CAGGATGTCAATCGGGAGCTGACAAACCAGCAGGAAGCGTCTGTAGAGAGGCAGCAGCAGCCGCCGCCAG
  AGACTTTTGATTTCAAAATCAAGTTTGCTGAGACCAAGGCTCATGCCAAGGCCATTGAGATGGAGTTGAG
  ACAGATGGAAGTTGCCCAGGCCAACCGGCACATGTCCCTGCTGACAGCCTTTATGCCTGACAGCTTCCTT
  CGGCCAGGTGGAGACCACGACTGTGTCCTGGTGCTGCTGCTCATGCCCCGACTCATTTGCAAGGCAGAGC
  TCATCCGGAAGCAGGCCCAGGAGAAGTTTGACCTGAGCGAGAACTGTTCGGAGCGGCCCGGGCTGCGGGG
  AGCTGCCGGGGAGCAGCTGAGCTTTGCTGCTGGACTGGTGTACTCGCTGAGTCTGCTGCAGGCCACGCTG
  CACCGCTATGAGCATGCCCTCTCTCAGTGCAGTGTGGACGTGTATAAGAAGGTCGGCAGCCTGTACCCCG
  AGATGAGCGCCCACGAGCGCTCCTTAGATTTCCTCATTGAGCTGCTGCACAAGGATCAGCTGGATGAGAC
  TGTCAACGTGGAGCCCCTCACCAAGGCCATCAAGTATTACCAGCATCTGTACAGCATCCACCTCGCTGAA
  CAACCCGAGGACTCCACCATGCAGCTGGCTGACCACATCAAGTTCACCCAGAGTGCCCTGGACTGCATGG
  GCGTGGAGGTGGGGCGGCTGCGTGCCTTCTTGCAGGGTGGGCAGGAGGCAACAGATATTGCCCTTCTTCT
  CCGAGACCTGGAAACATCATGTAGTGACACCCGTCAGTTCTGCAAGAAGATCCGAAGGCGGATGCCGGGG
  ACGGATGCTCCTGGGATCCCAGCAGCGCTGGCCTTTGGCTCACAGGTATCCGACACACTCCTGGACTGCA
  GGAAGCACTTGACGTGGGTGGTAGCTGTTCTGCAGGAGGTGGCAGCTGCAGCCGCCCAGCTTATTGCCCC
  CTTGGCAGAGAACGAGGGGCTGCCTGTGGCTGCACTGGAGGAGCTGGCCTTCAAAGCAAGCGAGCAGATC
  TACGGGAGCCCCTCCAGCAGCCCCTATGAGTGTCTACGCCAGTCATGCACCATCCTCATCAGCACGATGA
  ACAAGCTGGCCACAGCCATGCAAGAAGGCGAGTATGACGCAGAGCGACCCCCGAGCAAGCCTCCTCCGGT
  TGAACTTCGGGCTGCAGCCCTGCGTGCAGAGATCACAGATGCTGAAGGTCTGGGTTTGAAGCTTGAGGAT
  CGAGAGACAGTTATCAAGGAGTTAAAGAAGTCACTCAAGATTAAGGGAGAGGAGCTGAGTGAGGCCAACG
  TGCGGCTCAGCCTCCTGGAGAAGAAGTTGGACAGCGCTGCCAAGGATGCAGACGAGCGAATCGAGAAAGT
  TCAGACACGGCTGGACGAGACTCAGACCCTGCTGCGGAAGAAGGAGAAAGACTTTGAGGAGACAATGGAC
  GCACTCCAGGCTGACATCGACCAGCTGGAGGCAGAGAAGGCAGAGCTCAAGCAGCGCCTGAACAGCCAGT
  CCAAGCGCACAATCGAGGGGCTCCGGGGCCCCCCTCCGTCAGGCATCGCTACCCTGGTCTCTGGCATCGC
  TGGTGGGGGCGCTCCTGGGCAGGCTCCGGGCGCCTTGCCAGGCCCGGGGCTGGTGAAGGACTCCCCACTG
  CTGCTTCAGCAGATCTCTGCTATGAGGCTACACATCTCTCAGCTCCAGCATGAGAACAGCATCCTCAGAG
  GAGCCCAGATGAAGGCGTCCTTGGCAGCTCTGCCCCCTCTGCATGTTGCAAAGCTTTCCCTCCCACCCCA
  TGAGGGCCCCGGTGGTAACCTAGTGGCTGGGGCACTGTACCGCAAGACCAGCCAGCTCCTGGAGAAACTA
  AACCAGCTGAGTACCCACACCCACGTGGTGGATATCACTCGGAGCAGCCCAGCTGCCAAGAGCCCGTCAG
  CTCAGCTTATGGAACAAGTGGCTCAGCTCAAGTCCCTGAGTGACACCATTGAGAAGCTCAAGGATGAGGT
  CCTCAAGGAGACAGTGACTCAGCGCCCTGGAGCCACTGTGCCCACCGACTTTGCCACTTTCCCTTCATCT
  GCCTTCCTCAGGGCCAAGGAAGAGCAGCAAGATGACACAGTCTACATGGGCAAAGTGACCTTTTCATGCG
  CGGCAGGCCTAGGACAGCGACACCGCCTGGTGCTGACCCAGGAGCAGCTGCACCAGCTTCACAGTCGCCT
  CATCTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001198867
• Insert Size: 3720 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198867.1, NP_001185796.1
• RefSeq Size: 4172 bp
• RefSeq ORF: 3720 bp
• Locus ID: 13191
• Cytogenetics: 6 35.94 cM
• Gene Summary: Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule. Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon. Plays a role in metaphase spindle orientation. Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitement to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole. Plays a role in primary cilia formation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR,lacks multiple exons in the coding region, and uses an in-frame downstream start codon, compared to variant 1. Variant 3 encodes a protein (isoform 2) with a shorter N-terminus, compared to isoform 1.