Aff3 (NM_001290814) Mouse Untagged Clone

CAT#: MC229488

Aff3 (untagged) - Mouse AF4/FMR2 family, member 3 (Aff3)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Aff3
• Synonyms: 3222402O04Rik; A730046J16; LAF-4; Laf4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229488 representing NM_001290814
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGCTTCGACTTAGCCCTGCTCCAGGAATGGGACCTCGAGTCGCTGTGTGTCTATGAACCGGACA
  GGAATGCGTTACGGAGGAAAGAGCGAGAAAGAAGAAGTCAAGAAACACAGCAGGACAGTGGCAGCTTTAA
  CTCCGGTTACTCGCTCTTCAGCGAACCCTACAAGACGAACAAGGGGGATGAACTCTCCAACCGGATCCAG
  AATACATTAGGAAATTATGATGAGATGAAAGACTTTTTAACTGATAGATCCAACCAGAGTCATCTTGTTG
  GCGTCCCCAAGCCAGGTGTCCCTCAGACTCCCGTGAACAAGATAGATGAGCATTTTGGGGCAGAGTCGAG
  AGCCCAGCCCCAGCCCTCAACCGTCTGCAGCACTGCGTCCTCTACACCTGCAGCTGTCCCTGTGCAGCAG
  GGCAAGAGAGGTGCCATGGGCTGGCAGAAGGCTGGGCACCCACCGTCCGATGGCCAACAGAGAGCAGCGC
  AACAGGGCTCTCTCAGGACCTTGCTTGGAGATGGTGTTGGCAGACAGCAGACAAGGGCCAAGCAAGTGTG
  CAATATGGAGACGGGGCTTCAGACCCAGGAGAGGCCACCTGCAATGGCAGCCAAGCACGGCGGCAGTGGA
  CACTGTGTTCAGAACTTTCCCCCATCCCTGGCTTCAAAACCCAGCCTGGTGCAGCAGAAACCAACTGCCT
  ACGTGCGGCCAATGGATGGCCAGGACCAAGCTCCTGACGAATCTCCTAAGCTGAAGTCATCCACAGAAAC
  TGCTGTGCACTGCACAGCCTACAGAGGGGTCCCGGCCAACAAACCAGAGTCAGCCCGAGCCAAGGCCAAG
  CTTGCCAAGTTCAGCATCCCCAAGCAGGCAGAGGAGAGTAGATCTGGAGAAAACAACAGCTGTGTGGAAG
  AAATAATTCGGGAGATGACCTGGCTTCCTCCACTTTCTGCTATTCAAGCTCCTGCCAAAGTGGAACCAAG
  CAAGTTCCCATTCCCAAATAAGGACTCGCAACTTGTATCCTCTGGACACAGTAATCCAAAGAAAGCTGAT
  GCGGAGCCAGGGAGTCCAGACAATGGCGCATCGAACACTTCAACTCTGGAAGACGACCTTAAGCTAAGCA
  GTGATGACGAAGAGGGTGAGCAGCAGGCAGCCCAGAGAACAGCTCTCCGCGCTCTAGCTGACAGCTCCGT
  GGTTCAGCAGACCAACTGTAGAGGTTCGGCGCCATCCAGCAAGGGCGGCGGCAGCAGCAGCAGCAGCGGC
  GGCAGCAGCTCCTCCAGCGACTCAGAGAGCACTTCCGGCTCCGACTCCGAGACGGAGAGCAGCTCCAGCT
  CCAGCGAGAGCGAGGGCAGCAAGCCTCCGCACTGCTCCAGCCCCGAGGCCGAACCTGCGTCCTCTAACAA
  GTGGCAGCTGGATAAATGGCTAAACAAAGTTAATCCTCACAAGCCGCCAATTCTGATCCAAAATGAAAGC
  CACGGCCCGGAAAGGAATCAATACTACACGCCGCCGGTGAAAGATGAAGGGCAAGACTGCGGGAAACTTC
  CTGAGATCTGCCAAGCCAGCCTGAGGGACAAGGAGCTCAAGACCACCTGCAAGGAGGAGCAGAGGCCGAG
  GACCGCAAACAAGGCCCCTGGCAGCAAAAGTGTAAAGCAGAAGTCCCCACCCGCTGCTGTGGCTGTGACC
  GCAGCTGCCCTCCCGCCCGCGGTGCCCAGCGCACCCACGGAGAGCGCGCCTGCGCCCACCCGCAGGTCAG
  CAGGCAAGAAGCCCACCCGGCGCACCGAGAGGACCTCAGCCGGGGACGGTGCCAACTGCCACCGGCCTGA
  GGAGCCAGTGGCCCCAGACACGCTGGGGGCGAGCGTGGTGGGACCCCTAGAGCCCCCCAAAACCAGACCC
  TGCGGTAACAACAGGACAGGCCACCGCAAGGAGCTGCGCTCCTCCGTGACCTGTGAGAAGCGGCGCACGA
  GGGGGCTGAGCAGAATCGTCCCCAAATCCAAGGAGTTTATTGAAACAGAATCTTCGTCTTCCTCTTCCTC
  CTCGGACTCAGACCTGGAGTCAGAGCAAGAGGAGTACGTCCTGTCCAAGGCTCCGACCACCACTGGGAGC
  GAGCAGCGGCTGAAGGAGGCCGCCAGCAGCAACAACAACAGCAACAGCAACAGCAGCACCTCCCGGGCTT
  CTGTGGGCTCCATCAACGCCAGGACCACCAGTGACATTGCCAAGGAGCTAGAGGAACAGTTCTACACGCT
  GGTCCCCTTTGGCAGGAATGAACTACTATCTCCGCTAAAGGACAGCGATGAGGTCAGGTCTCTCTGGGTC
  AAAATTGACCTGACCCTCCTGTCCAGGATCCCAGAACACCTGTCCCAGGAGCCTGGGGTTTTGAGTGCTC
  CTTCGGCCAAGGACACTGATAGTGCCCCAGCCAGTCATGCATTAGACGCTCCTGCTGAAAAGACTTTGCC
  AAAATCCAAGAGGAAACGCAAGTGCGACAACGAAGATGACTACAGGGAGATAAAGAAGGTCCAGGGAAGG
  AAGGAGAGTGCCTCACGCCTCGCCGCCTCCACTAATAACACCTTGTCCGGCAATCACTGCAATGTGAACG
  TCAACAGCTTGGCAATACCAATAAACAAAAATGAAAAGATGCTCCGGTCGCCCACTTCGCCACTCTCAGA
  CACATGTAAACACAAATACGCCAGCGAGGACCTGACTTCTTCCAGCCGACCTCACGGCAACGGGTTGTTG
  ACTTCAGCGTCTTCCAACAAAGAGCCAAAGGCTGAGAGTCAGCTGCAGACGATTGCTGGGGACCTCACGA
  AGGCATCTCACAACAGTTCTGAAAATGGCACCCTCCATAGCAAGTCACGGCCCCAGACGGAGCCATGGTC
  TCCAGGTTCCAATGGCCACAGGGACTGCAAGAGACAGAAACTGATCTTCGATGACATGCCTCGAAGTGCA
  GATTATTTTATGCGAGAAGCCAAGCGGATGAAACATAAAGCAGACGCCATGGTGGAGAAGTTCGGGAAGG
  CCCTGAACTATGCCGAAGCTGCCTTGTCCTTCATCGAGTGCGGAAACGCAATGGAACAAGGGCCAATGGA
  ATCCAAGTCTCCCTACACCATGTACTCGGAGACAGTGGAGCTCATCAGGTATGCTATGAGACTAAAAACC
  CACTCAGGCCCCAATGCCACACCAGAGGACAAACAGTTAGCTGCGCTTTGTTACCGATGCCTGGCTCTCC
  TATACTGGCGGATGTTTCGACTCAAAAGAGACCATGCTGTAAAATATTCAAAAGCACTTATCGACTATTT
  CAAGAATTCATCGAAAGCTGCCCAAGCCCCATCCCCATGGGGGTCCAGTGGCAAGAGCACTGGAAGCCCA
  TCCCCCATGTCTCCCAACCCTTCTCCCGCCAGCTCCGTGGGGTCTCAGGGCAGCCTCTCCAGCTCCAGCG
  GCCTGTCCCCATCGACCATCGTCAGCATCCCCCAGCGCATTCACCAGATGGCGGCCAATCACGTCAGCAT
  CACCAACAGCATCCTCCACAGCTATGACTACTGGGAGATGGCTGATAACCTGGCCAAGGAAAACAGAGAG
  TTCTTCAATGACCTGGATTTGCTCATGGGACCTGTCACCCTGCACAGCAGCATGGAACACCTGGTCCAGT
  ACTCCCAGCAAGGCCTGCATTGGCTTCGGAGCAGCGTACACCTGTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290814
• Insert Size: 3690 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290814.1, NP_001277743.1
• RefSeq Size: 7251 bp
• RefSeq ORF: 3690 bp
• Locus ID: 16764
• Cytogenetics: 1 16.58 cM