Brpf1 (NM_001282128) Mouse Untagged Clone

CAT#: MC229482

Brpf1 (untagged) - Mouse bromodomain and PHD finger containing, 1 (Brpf1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Brpf1
• Synonyms: 4833438B11Rik; 4930540D11Rik; Brpf2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229482 representing NM_001282128
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGTGGACTTTGACGTGAAGACCTTCTGCCACAATTTGCGGGCAACTAAGCCACCATATGAGTGCC
  CTGTGGAGACTTGCCGCAAGGTTTACAAGAGTTACAGTGGTATCGAGTACCACCTGTACCACTATGACCA
  CGACAGCCCACCACCCCCACAGCAGACCCCACTGCGCAAGCACAAAAAGAAAGGGCGCCAGTCACGACCA
  GCCAACAAGCAGTCACCCAGCCCCTCTGAAGTCTCACAGTCACCAGGCCGAGAGGTGATGAGCTATGCTC
  AGGCCCAGCGCATGGTAGAAGTGGACCTTCATGGCCGTGTCCACCGAATCAGCATCTTTGACAACTTGGA
  TGTGGTGTCAGAGGATGAGGAGGCCCCTGAGGAGGCCCCTGAGAATGGCAGCAACAAGGAAAACACTGAG
  ACACCTGCGGCTACACCTAAGTCAGGCAAGCATAAGAACAAGGAGAAACGAAAAGACTCTAACCACCACC
  ATCACAGCGCTCCTGCCAGTGCTGCTCCCAAATTGCCTGAGGTGGTGTATCGTGAGCTAGAGCAAGATAC
  CCCTGACGCACCACCCCGGCCCACTTCCTACTACCGGTACATCGAGAAATCTGCAGAGGAGCTGGATGAG
  GAGGTGGAGTATGACATGGATGAAGAGGACTATATCTGGCTGGATATCATGAATGAGCGGCGGAAGACTG
  AGGGTGTAAGTCCCATCCCACAAGAGATCTTTGAGTACTTAATGGACCGTTTGGAGAAGGAGTCGTACTT
  TGAGAGTCACAATAAAGGTGACCCCAATGCACTAGTGGATGAAGATGCCGTGTGCTGTATCTGCAATGAT
  GGCGAGTGCCAGAACAGCAATGTTATCCTCTTCTGTGACATGTGTAACTTGGCTGTGCACCAGGAGTGCT
  ACGGTGTCCCCTATATCCCTGAAGGCCAGTGGCTGTGCCGCCGTTGCCTGCAGTCACCTTCTCGTGCAGT
  GGATTGTGCTCTGTGCCCCAATAAGGGTGGTGCCTTCAAGCAGACAGATGATGGCCGCTGGGCCCACGTG
  GTGTGTGCCTTGTGGATCCCTGAGGTTTGCTTTGCCAACACAGTCTTCCTAGAACCTATTGACAGCATTG
  AGCACATCCCACCAGCTCGGTGGAAGCTCACCTGCTACATTTGTAAACAGCGGGGCTCTGGAGCCTGCAT
  CCAGTGCCATAAGGCCAATTGCTACACAGCCTTCCATGTGACATGTGCCCAACAAGCTGGCCTTTACATG
  AAGATGGAACCTGTGCGGGAGACAGGTGCCAATGGTACCTCCTTTAGCGTCCGCAAGACAGCCTACTGTG
  ACATCCACACACCCCCAGGTTCTGCTCGTCGCCTGCCTGCCCTATCCCACAGTGAGGGTGAGGAAGAAGA
  GGATGAAGAAGAAGATGAGGGTAAAAGCTGGAGCTCAGAGAAGGTCAAGAAGGCCAAGGCCAAGTCTCGG
  ATTAAGATGAAGAAAGCTCGGAAGATCTTGGCAGAGAAGAGGGCAGCAGCACCTGTGGTGTCCGTGCCCT
  GCATCCCGCCACACAGGCTCAGTAAGATCACCAACCGCCTGACCATCCAGAGGAAGAGCCAGTTCATGCA
  GAGGCTGCACAGCTACTGGACTCTGAAACGACAATCACGGAATGGGGTCCCACTACTCAGGCGCCTACAA
  ACACACCTTCAGTCTCAGAGGAACTGTGAACAAGTTGGGAGAGATTCTGATGATAAAAACTGGGCCCTCA
  AAGAACAGCTCAAGTCCTGGCAGAGACTGCGGCATGACCTGGAGCGAGCTCGGCTGCTGGTGGAGCTGAT
  CCGCAAGCGAGAGAAACTAAAAAGGGAGACGATCAAGATCCAGCAGATTGCCATGGAGATGCAGCTGACC
  CCTTTCCTCATCCTCCTCCGAAAAACCTTGGAGCAGCTCCAAGAGAAGGACACAGGCAACATCTTCAGCG
  AGCCGGTCCCTCTGTCTGAGGTAACCGAATTGGACGAAGTACCCGACTACCTAGACCACATCAAAAAGCC
  CATGGACTTTTTCACCATGAAGCAGAACTTGGAGGCTTACCGCTACTTGAACTTTGATGATTTTGAGGAG
  GACTTCAACCTCATTGTCAGCAACTGCCTAAAGTATAATGCCAAGGACACCATCTTCTACAGGGCAGCAG
  TGCGACTCCGTGAGCAGGGTGGTGCTGTGCTCCGTCAAGCCCGGCGACAGGCAGAAAAAATGGGCATTGA
  CTTTGAGACGGGCATGCATATCCCTCACAACCTGGCCGGAGATGAGGTCTCACACCACACTGAAGATGTA
  GAGGAAGAACGGCTGGTCCTGCTGGAGAACCAGAAACACCTGCCAGTAGAAGAACAGCTGAAGTTGTTGC
  TGGAGAGGCTGGATGAAGTCAATGCCAGCAAGCAGAGTGTGGGCCGCTCTCGGCGTGCAAAAATGATCAA
  GAAGGAGATGACAGCATTGCGGCGGAAGCTTGCTCACCAGCGGGAGACTGGCCGGGATGGGCCTGAGCGT
  CATGGCCCCTCAGGTCGGGGCAATCTGACACCCCACCCAGCAGCCTGTGACAAAGATGGACAGACCGACA
  GTGCTGCAGAAGAGAGCAGCAGCCAGGAAACAAGCAAAGGCCTGGGTCCCAACATGTCCTCAACCCCCGC
  ACATGAGGTGGGCAGGAGAACCTCAGTTCTGTTCTCCAAAAAGAACCCGAAGACAGCTGGACCGCCCAAG
  AGGCCGGGCCGGCCCCCAAAAAACCGGGAGAGCCAGATGACCCCCAGCCACGGAGGCAGTCCTGTGGGGC
  CCCCTCAGCTCCCCATCATGGGCTCCCTACGTCAGCGCAAGCGGGGTAGGAGCCCCCGGCCCAGTTCAAG
  CTCAGACAGCGACAGTGATAAGTCCACAGAAGATCCCCCAATGGACTTACCAGCCAATGGCTTCAGCAGT
  GGGAACCAGCCAGTGAAGAAGAGTTTCTTGGTGTACCGTAATGACTGCAACCTTCCCCGAAGCAGCTCAG
  ACTCTGAGTCCAGCAGCAGCAGCAGCAGCAGTGCAGCCTCAGACCGGACCAGCACAACTCCCTCAAAACA
  AGGCAGGGGCAAGCCCTCTTTCTCTCGGGGCACATTCCCAGAAGACAGTAGTGAAGATACCTCAGGCACT
  GAGAATGAGGCCTACTCCGTGGGCACTGGCCGCGGCGTGGGCCACAGCATGGTAAGAAAGAGTCTGGGTC
  GAGGAGCTGGCTGGCTGTCAGAGGATGAGGACTCCCCGTTGGATGCTCTGGACCTCGTGTGGGCCAAATG
  CCGAGGCTATCCATCATACCCAGCTCTGATCATTGATCCAAAGATGCCCCGAGAAGGTATGTTCCACCAT
  GGGGTTCCTATCCCTGTACCACCACTGGAGGTTCTAAAACTTGGGGAACAAATGACACAGGAAGCCCGAG
  AGCATCTCTACCTCGTTCTCTTCTTTGACAACAAACGAACCTGGCAGTGGCTGCCCCGGACTAAGCTTGT
  TCCTCTGGGTGTGAACCAGGATCTAGACAAAGAGAAGATGCTGGAGGGCCGCAAGTCCAACATCCGCAAG
  TCAGTGCAGATTGCTTACCACAGGGCTCTGCAGCACCGAAGCAAGGTGCAGGGTGAGCAGAGCAGCGAGA
  CCAGCGATAGTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282128
• Insert Size: 3657 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282128.1, NP_001269057.1
• RefSeq Size: 4796 bp
• RefSeq ORF: 3657 bp
• Locus ID: 78783
• Cytogenetics: 6 E3
• Gene Summary: Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Preferentially mediates histone H3-K23 acetylation (PubMed:27939640). Positively regulates the transcription of RUNX1 and RUNX2 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) uses an alternate in-frame splice site, and lacks an in-frame segment in the central coding region, compared to variant 1. The encoded isoform (4) is shorter, compared to isoform 1.