Fmn1 (NM_001285459) Mouse Untagged Clone

CAT#: MC229468

Fmn1 (untagged) - Mouse formin 1 (Fmn1), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fmn1
• Synonyms: Fmn; formin-1; ld
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229468 representing NM_001285459
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGGTTGGTAACTCTCTCAGCAGCAGAGATGTGCTGGAACCAGACAAATCCGAAGCCGGACTTG
  AGATGGCTCAGTCGATCCTGAGTAAATTCTCTATGAAATCCCTGTTTGGATTTACAAATAAATTGGACTC
  CTTGGAGCCTGAAGAGGAAGATGCTGTGCTGAAGGCATTTCGCAGTTTAGAGGGGGATCCCGCTCCTGAG
  CGAGGTGATCCCAGCAAAGGCTCAGATCAGCCACAGGCAGAGGCTCCAGTTCCACCTGATCTTAAAAATG
  ATGGAAAGTCAGCAAGGGCGGAGACAGGATCTGAGGGAAGTCAGGGGAAAGGCAGATCAAACACCTCTTC
  CCCTGGTTATGAGCTTTCGCCTGCTACAGTGAGCGTGGACAATGAGGAAGTCATTTGGGTCCGTGGGACC
  CTGGTTCACACCACCAGTGATTCTGACTCTGAAGATGGAGACCAGGAGGCAGAAGAAGAAAGTAGCCTTG
  ATACCCAAAAGCCCACCACTGTTGTTTTATGTGAACCTTCTCAAGAGCCCAAAGACAGAGCAGGGGATTC
  CGAAGAAAATACAGACACTGGGAACACTGATGATACAGAACTCTGTGCTGAAGAGTCTCAGAGAACTTTA
  CCTGAGACAAGTAGCAAACTGGAACTTGGTGGTGATGGTAGCCATCCGGCAGAGCACAGCCCCAGGCAGG
  ACCAGGCTGCAGAAGAAGGGTCTCAAATCCCACCCGCAGCAACAGACCAGACTGTAGGTGCCCTTGCGAG
  CACTGTTTCTAAAAGAGAAGCTCCGGAAGAGAAGCCCTTCCAGCTTCCAGCTTTCTTCAGTGGGCTCCGT
  GTGCTGAAGAAGGGGGCTACCGCAGAGGCAGGGGAGACCATCACTGAAATCAAACCCAAGGACGGGGACC
  TGGCTTTGCTCAAGTTGACCCAGCGTGTTCAGAAGTCTCTGGGACAGGGAGGCCCACAGACAGTGAAGAG
  CCCAGGTAGAGCAACTGATCCGAAGGCCACTCCCACTCTCCTGGAGCAGCTATCACAGCTGCTCAACATT
  GACATGCCAAGGACCGAACAGAAGGAAGCGGACCCCGAGTTTCATGGTGCGGATGAGATGGGTTACAGTA
  CTGACCAGGAGAGCCACAAGAGCCCCAGAGATGCCCACGTCCAGGGTGGTCAGGTGAAGGCAAGAACACC
  AGAGACTGCCTTGGAGGCATTTAAGGCCTTATTCATCCGTCCCCCCAAAAAGGGGAGCACAGCTGACACT
  TCTGAGCTGGAAGCTCTTAAGCGAAAGATGAAGCATGAGAAGGAGTCACTACGCGCTGTGTTCGAGAGGT
  CCAAGTCTCGGCCGGCAGACAGCCCGTCTGATCCCAAAAGCCCTGACCAGAGCCCCACAGAGCAGGACGA
  CAGGACTCCAGGCAGACTCCAAGCTGTCTGGCCACCCCCAAAGACAAAGGACACAGAAGAAAAAGTGGGA
  CTGAAGTACACTGAAGCAGAATACCAGGCTGCTATCCTACATCTGAAGAGGGAGCACAAAGAAGAAATCG
  AAACCCTGCAGGCTCAGTTTGAACTCAAGACATTTCACATCCGGGGTGAGCACGCATTAGTAACAGCAAG
  ACTTGAAGAAGCCATTGAAAATCTCAAGCAACAGTTAGGAAAGCGCCGGGAAGGATGTGAAGAGATGAGA
  GATGTGTGCATTTCCACAGATGATGACTGCTCTCCAAAGGCTTTCAGAAATGTGTGCATCCAGACAGACA
  GAGAGACCTTCCTCAAGCCCTGTGATGCTGAAAGCAAAGCAACCAGAAGCAGCCAGATAGTACCCAAGAA
  GCTGACTATCTCCTTAACCCAGCTCTCTCCCTCAAAAGACAGCAAAGACATCCACGCCCCATTCCAGACA
  AGAGAAGGCACCTCCTCATCTAGTCAGCATAAGATATCCCCTCCAGCTCCCCCCACGCCACCACCACTCC
  CTCCACCTCTCATTCCTCCACCTCCGCCCCTCCCACCTGGACTTGGACCTTTGCCTCCAGCACCACCCAT
  ACCACCTGTGTGTCCTGTGTCGCCACCGCCACCTCCTCCGCCTCCTCCTCCAACTCCCGTGCCTCCAAGT
  GATGGACCACCACCACCACCACCTCCTCCACCACCACTTCCCAATGTCCTAGCTCTTCCTAACAGTGGAG
  GTCCTCCTCCTCCTCCACCTCCTCCTCCGCCAGGACTTGCACCCCCACCTCCTCCTGGACTGTCCTTTGG
  ACTCAGCTCTTCTTCCAGCCAGTATCCTCGTAAACCAGCCATTGAGCCCAGCTGTCCTATGAAACCTTTG
  TATTGGACCAGAATACAAATAAATGATAAAAGCCAAGACGCCGCACCAACTTTATGGGACTCCTTAGAAG
  AGCCTCATATTAGGGACACAAGTGAATTTGAATATTTATTCTCCAAGGACACAACTCAACAGAAGAAAAA
  ACCCCTGTCAGAGGCCTACGAAAAGAAGAACAAAGTCAAAAAGATCATCAAGTTATTGGATGGAAAGCGA
  TCTCAAACTGTGGGAATCTTGATATCTAGTTTACATCTAGAAATGAAAGATATTCAACAGGCCATATTTA
  CTGTGGATGACTCCGTGGTTGACCTGGAGACCTTAGCAGCCTTATATGAAAATCGAGCCCAGGAGGATGA
  ACTGACTAAAATAAGAAAGTACTATGAGACATCCAAAGAAGAAGACTTGAAACTGCTGGACAAACCTGAA
  CAATTTTTGCATGAGTTAGCCCAGATTCCCAATTTTGCCGAACGTGCCCAGTGCATAATCTTCAGGGCTG
  TATTTTCTGAGGGTATCACATCCTTACATCGAAAAGTAGAGATTGTCACACGGGCCTCGAAGGGCTTGTT
  GCACATGAAGAGTGTGAAGGATATCTTAGCTCTCATTCTGGCTTTTGGAAACTACATGAATGGAGGAAAC
  AGGACGCGAGGGCAAGCAGACGGATATAGTTTAGAAATTCTGCCCAAACTCAAAGACGTCAAAAGTCGGG
  ACAATGGGATGAATCTGGTGGACTATGTTGTGAAGTACTACCTGCGATACTATGATCAGGAAGCTGGAAC
  AGACAAGAGTGTTTTCCCGCTGCCTGAACCACAGGATTTCTTCCTGGCCTCTCAAGTCAAGTTTGAAGAC
  CTCCTAAAGGATTTGAGGAAGCTGAAGCGTCAACTAGAAGCAAGTGAGCAACAGATGAAGCTGGTGTGCA
  AGGAGTCCCCAAGGGAGTACCTGCAGCCTTTCAAGGACAAACTGGAGGAGTTCTTCAAGAAAGCCAAAAA
  AGAGCACAAGATGGAAGAAAGTCACTTGGAGAATGCACAGAAAAGTTTTGAAACAACAGTGGGATATTTT
  GGAATGAAGCCAAAGACTGGAGAGAAGGAGGTCACCCCCAGCTATGTGTTTATGGTGTGGTTTGAGTTCT
  GCAGTGACTTCAAGACCATTTGGAAGCGGGAGAGTAAGAACATATCTAAAGAAAGATTGAAAATGGCTCA
  GGCATCCGTCAGCAAACTGACATCAGAGAAGAAAGTGGAGACAAAGAAAATCAATCCCACCGCTAGTCTG
  AAAGAAAGACTGCGTCAGAAGGAAGCCAGCGTGGCCACCAACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285459
• Insert Size: 3615 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285459.1, NP_001272388.1
• RefSeq Size: 11147 bp
• RefSeq ORF: 3615 bp
• Locus ID: 14260
• UniProt ID: Q05860
• Cytogenetics: 2 57.3 cM
• Gene Summary: Plays a role in the formation of adherens junction and the polymerization of linear actin cables.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) represents the use of an alternate promoter, differs in the 5' UTR, lacks 5' coding sequence and an alternate in-frame exon in the 3' coding region, and uses an alternate start codon compared to variant 1 (PMID: 1339380). The resulting protein (isoform 4, also known as IV) has a distinct N-terminus and is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.