Dhx30 (NM_001252682) Mouse Untagged Clone

CAT#: MC229462

Dhx30 (untagged) - Mouse DEAH (Asp-Glu-Ala-His) box polypeptide 30 (Dhx30), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dhx30
• Synonyms: 2810477H02Rik; C130058C04Rik; Ddx30; HELG; Ret-CoR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229462 representing NM_001252682
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAGCTTGGACTCATTCAGAAAAGATCGGACCCAGCACAGGCAGCGTCAATGCAAGCTTCCCCCAC
  CCCGTCTTCCACCCATGTGTGTCAACCCTGCCCCTGGAGGGACCATCACTCGAGCTTCTAGGGACCTATT
  AAAAGAGTTTCCGCAGCCTAAAAACCTTCTCAACAGCGTGATTGGAAGAGCCCTTGGCATCTCACATGCA
  AAAGACAAGTTAGTCTATGTGCACACGAATGGACCGAAGAAAAAGAAAGTCACCCTGCACATAAAGTGGC
  CCAAGAGCGTGGAGGTGGAAGGCTATGGCAGCAAGAAGATTGATGCTGAGCGTCAGGCTGCAGCAGCTGC
  CTGCCAACTCTTCAAGGGCTGGGGTCTGCTGGGACCACGGAATGAGCTGTTTGATGCAGCTAAATACCGA
  GTGCTAGCTGATCGTTTTGGGTCTCCAGCTGACAGCTGGTGGCGCCCAGAACCCACCATGCCTCCAACTT
  CCTGGCGGCAGCTGAATCCTGAGAACATTCGGCCAGGGGGTCCTGCAGGACTATCCCGATCCTTAGGCCG
  AGAGGAAGAGGAAGATGAGGAGGAAGAGCTAGAAGAGGGGACCATTGATGTGACAGAGTTTCTGTCTATG
  ACCCAGCAAGACTCCCACAACCCACTCAGGGACTCAAGGGGGGGCTCCTTTGAAATGACAGATGATGACA
  GTGCTATCAGAGCTCTGACCCAGTTTCCACTTCCCAAGAACCTCCTGGCCAAAGTGATTCAGATTGCAAC
  CTCCTCCTCCACAGCTAAGAATCTCATGCAGTTCCATACTGTGGGTACCAAGACCAAGCTGGCTACACTC
  ACTCTGCTCTGGCCCTGTCCCATGACCTTTGTTGCCAAAGGGCGACGCAAAGCTGAGGCTGAGAATAAGG
  CAGCAGCCTTGGCTTGCAAGAAACTGAAGAGCCTTGGCCTTGTGGACAGGAACAATGAGCCGCTTACCCA
  TGCCATGTACAACCTGGCCTCCCTGCGTGAGTTGGGTGAGACCCAGCGCCGGCCATGTACCATCCAGGTG
  CCTGAGCCCATCCTTCGCAAGATAGAGGCCTTCCTGAGTCATTACCCGGTGGACAGCTCATGGATTTCCC
  CAGAACTCCGACTGCAGAGTGATGACATCTTGCCCTTAGGCAAGGACTCAGGGCCCTTGAGTGACCCTAT
  CACAGGCAAGCCATACATGCCCCTGTCAGAAGCAGAGGAGGTGCGCTTAAGCCAGAGCCTGCTAGAGCTG
  TGGCGGAGGAGAGGGCCGATCTGGCAGGAGGCCCCGCAGCTACCTGTAGACCCTCATCGGGACACTATCC
  TCAGTGCCATTGAACAACACCCAGTGGTAGTCATCTCTGGGGACACAGGCTGTGGGAAGACCACACGTAT
  CCCTCAGCTGCTATTGGAGCGCTATGTGACTGAGGGTCGAGGTGCCCGCTGCAATGTGATCATCACACAA
  CCTCGCCGGATCTCAGCTGTGTCTGTGGCACAGCGGGTCAGCCATGAACTGGGCCCCTCCTTGCGCCGGA
  ATGTGGGCTTCCAGGTACGCTTGGAAAGTAAGCCCCCAGCCCGAGGTGGGGCGCTGCTCTTCTGCACTGT
  GGGTATCCTGCTTCGGAAGCTGCAGAGCAACCCCAGCCTGGAGGGTGTGAGCCATGTCATTGTGGATGAG
  GTCCATGAGCGGGATGTGAACACAGACTTCCTGCTGATTCTGCTCAAGGGCCTGCAGCGGCTCAACCCAG
  CTCTTCGGCTGGTGCTCATGAGTGCTACAGGAGATAATGAGCGCTTTTCCCGATACTTTGGGGGCTGCCC
  TGTTATCAAGGTACCTGGCTTCATGTATCCCGTCAAGGAACACTACCTGGAGGACATCTTGGCCAAGCTG
  GGTAAACATCAGTACCCACACCGGCATCGGCACCATGAGTCGGAAGATGAATGTGCACTTGATTTGGACC
  TTGTGACTGACCTGGTTCTGCATATCGATGCCCGTGGGGAACCAGGTGGGATCCTGTGCTTTCTACCTGG
  CTGGCAAGAAATCAAAGGAGTGCAACAACGGCTCCAGGAGGCCCTGGGCATGCATGAAAGCAAGTACCTC
  ATCTTACCAGTGCACTCCAATATCCCCATGATGGACCAGAAGGCCATATTCCAACAGCCTCCACTTGGGG
  TACGCAAGATTGTATTGGCCACCAACATTGCTGAAACCTCCATCACAGTTAACGACATTGTACATGTCGT
  GGACAGCGGTCTGCACAAGGAGGAACGCTATGACCTGAAGACCAAGGTGTCCTGCCTGGAGACTGTGTGG
  GTGTCGAGAGCAAATGTCATTCAGCGCCGGGGCAGGGCAGGCCGCTGCCAGTCAGGTTTTGCCTACCACT
  TGTTCCCGAGGAGCCGGCTGGAGAAAATGGTTCCTTTCCAAGTGCCAGAGATCCTGCGCACACCTCTTGA
  GAACCTGGTGCTGCAAGCCAAAATCCATATGCCTGAGAAGACGGCAGTGGAGTTCCTCTCTAAGGCTGTG
  GACAGTCCAAATATCAAGGCAGTGGATGAGGCCGTGATCCTGCTCCAGGAGATTGGGGTGCTGGACCAGC
  GGGAGTATCTGACCACCTTGGGACAGCGCCTGGCCCACATCTCTACTGACCCCCGACTGGCCAAGGCCAT
  AGTGTTGGCTGCCATCTTCCGTTGCCTGCACCCACTGCTGGTGGTTGTTTCCTGCCTTACCCGGGACCCC
  TTCAGCAGCAGTTTGCAGAACCGGGCAGAAGTAGACAAGGTGAAGGCATTGCTGAGCCATGACAGTGGCA
  GTGACCATTTGGCCTTCGTGCGGGCTGTGGCTGGCTGGGAGGAGGTACTGCGCTGGCAGGACCGTACCTC
  CAGGGAAAACTACCTGGAAGAAAACCTTCTGTATGCCCCCAGCTTGCGCTTCATCCACGGGCTCATCAAG
  CAGTTCTCAGAGAACATTTATGAGGCTTTCCTAGTGGGGAAGCCCTCTGACTGCACACTGCCCTCTGCTC
  AGTGCAATGAGTACAGCGAGGAAGAGGAGCTGGTGAAGGGTGTGCTGATGGCTGGCCTCTACCCCAACCT
  CATCCAGGTGAGACAAGGTAAGGTTACTCGGCAAGGCAAGTTCAAACCCAACAGTGTCACTTACAGGACC
  AAATCTGGCAACATCTTGCTGCATAAGTCAACCATTAACAGGGAGGCTACCCGGTTACGGAGCCGATGGC
  TGACATATTTCATGGCCGTCAAGTCCAATGGTAGCGTCTTTGTTCGAGATTCCTCCCAGGTGCACCCACT
  AGCTGTGTTGCTCCTAACAGATGGGGACGTGCACATCCGAGATGATGGGCGTCGGGCCACCATCTCATTG
  AGTGACAGCGACCTGCTTCGGCTGGAAGGTGATTCACGAACTGTGCGGTTGCTAAGGGAGTTTCGTCGAG
  CCCTAGGACGGATGGTGGAGCGGAGCCTCCGCAGCGAGTTAGCTGCACTTCCTCTTAGTGTGCAGCAAGA
  ACACGGGCAGCTGCTTGCACTGCTGGCAGAGTTGCTTCGAGGACCTTGTGGCAGCTTTGATATGCGCAAG
  ACAGCTGATGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252682
• Insert Size: 3585 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252682.1, NP_001239611.1
• RefSeq Size: 4228 bp
• RefSeq ORF: 3585 bp
• Locus ID: 72831
• UniProt ID: Q99PU8
• Cytogenetics: 9 F2
• Gene Summary: RNA-dependent helicase (PubMed:25219788). Plays an important role in the assembly of the mitochondrial large ribosomal subunit (By similarity). Required for optimal function of the zinc-finger antiviral protein ZC3HAV1 (By similarity). Associates with mitochondrial DNA (By similarity). Involved in nervous system development and differentiation through its involvement in the up-regulation of a number of genes which are required for neurogenesis, including GSC, NCAM1, neurogenin, and NEUROD (PubMed:25219788).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the shortest isoform (1).