Anks1 (NM_001286040) Mouse Untagged Clone

CAT#: MC229456

Anks1 (untagged) - Mouse ankyrin repeat and SAM domain containing 1 (Anks1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Anks1
• Synonyms: Anks1a; mKIAA0229
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229456 representing NM_001286040
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAGGAGCAGGAGCTGCTGGAGGCGGCCCGCACCGGGCACCTCCCGGCCGTGGAGAAGCTGCTGT
  CCGGGAAGCGGCTCTCCTCCGGCTTCGGCGGCGGCGGCGGCGGCTCAGGCAGCGGCGGCGGCTCCGGGGG
  CGGCGGCCTCGGTTCTTCTAGCCACCCGCTCTCCAGTCTGCTGAGCATGTGGAGAGGGCCGAACGTGAAC
  TGTGTAGATAGCACCGGCTACACGCCACTACACCACGCTGCTTTGAATGGACACAGGGATGTGGTCGAGG
  TCCTTCTGAGGAACGACGCGCTGACCAACGTTGCTGACTCGAAAGGCTGCTACCCGCTGCACTTGGCAGC
  GTGGAAAGGAGACGCCCAGATAGTACGTCTGCTCATCCAGCAAGGGCCCTCACACACCAGAGTCAACGAA
  CAGAATGCTCTTGAGATCAGAGAACTCAAAAAGTACGGCCCCTTTGACCCTTATATCAATGCCAAGAACA
  ACGACAATGAGACGGCCCTGCACTGCGCGGCCCAGTACGGCCACACGGAGGTCGTGAAGGCTCTGCTGGA
  GGAGCTCACGGACCCCACCATGCGCAACAACAAGTTTGAAACGCCCCTGGACTTGGCGGCGCTGTACGGG
  CGGCTGGAGGTGGTGAAGCTGCTGCTGGGTGCACACCCCAATCTCCTGAGCTGCAGCACTCGGAAACACA
  CGCCCCTGCACTTGGCAGCAAGGAATGGCCACAAGGCCGTGGTGCAGGTCCTGCTGGACGCCGGCATGGA
  TAGCAACTATCAGACGGAGATGGGCAGTGCTTTGCATGAGGCTGCTTTGTTTGGCAAGACCGATGTGGTG
  CAAATCCTGCTGGCTGCAGGAATCGATGTTAACATAAAGGATAACCGTGGCCTGACTGCCCTTGACACTG
  TCCGGGATCTGCCCTCTCAGAAGAGCCAGCAGATAGCCGCCCTGATTGAAGATCACATGACCGGAAAAAG
  AAGCGTGAAAGAAGTGGACAGGACCTCCACAGCCCAGCTACCTCTCCTCTCCAACACAGACGCCATAGCA
  CCAATGTCTCAGGGGAGTATGGAGAAGACAGTGACTGAGCTGATCCTTCATTTTGACACACATGCTGACG
  AGGAGGGCCCCTACGAGGCACTGTACAACGCTGTGTCCTGCCATTCCTTGGACAGCACGGCCAGTGGACG
  ATCATCAGACAGAGACTCCATGAACAAGGAGGCCGAGGCCACCGGAACAAGGGCTGCTGGAGTGAGGCCT
  AGGGAACGTCCGCCGCCTCCAGCAAAACCGCCACCCGATGAAGAGGAGGAAGAACGCGTAGATAAGAAGT
  ATTTTCCCTTGGCAGCCTCTGAGGGCCTGGCTGTGAGACCCAGGATTCAGAGTAGTGCTCCCCAGGAAGA
  GGAGGAGCACCCATATGAGCTGCTGCTCACGGCAGAAACCAAGAAGCTGGGGACCACAGACGGGAGGACT
  GAAGACCACAGGCAGAGCGGCAGCGGCCGGAGCCAGGACTCTGTGGAGGGGCAGGACGGGCAGGTCCCAG
  AGCAGTTCTCAGGTCTCCTCCACGGCTCCTCCCCAGTGTGTGAGGTGGGGCAGGACCCTTTCCAGCTGCT
  CACGGCCCCCAGCCAGAGCCACCCGGAGTCCTCCCAGCAGGACGCCTGCCACGAGGCCAGCATGCAGCTA
  GAGGAGCCAGGCGTGCAAGGCACTGAACCCCCGCAGCCCGGCGTCCCTGACCAAAGCAAGAGAGTGGGCT
  TGCCGGCAGGCCTGACAGCCCTGGCCAGCAGAACATACCTCGATGCATTGACTCACACAGTACCTCTGCG
  CCCTGCTGGTGCCGAGGAAGAAGACCAGAGCGGGCCCAGAAGCAGAGCCCCTCCCACCAGCAAGCCCAAA
  GCTGAGCTCAAACTCAGCCGCAGTTTGTCTAAGTCTGACTCTGACCTCCTGACCTGCTCCCCCACGGAGG
  ACGCCACAATGGGGAGTCGCAGCGAGTCCTTGTCCAACTGCAGCATCGGGAAGAAGAGGTTGGAGAAATC
  ACCTTCCTTTGCCTCAGAGTGGGATGAGATTGAGAAAATCATGAGCTCCATTGGGGAAGGGATTGACTTC
  TCTCAGGAGCAGCAGAAGATCTCAGGTTCTCGGACGCTGGAGCAGAGCGTCGGGGAGTGGCTGGAGTCGA
  TTGGCCTGCAGCAGTACGAGAGCAAGCTGCTGCTGAACGGCTTTGATGATGTCCGCTTCCTGGGGTCTAA
  TGTGATGGAAGAGCAGGACCTGAGGGAGATTGGCATCAGTGACCCACAGCACCGGCGGAAGCTGCTGCAG
  GCTGCACGCTCCTTGCCCAAGGTGAAGGCCCTTGGCTATGACGGGGTCAGCCCCACCTCAGTGCCCTCAT
  GGCTGGACTCGCTGGGGCTGCAGGACTATGTCCACTCTTTCCTGTCCAGTGGCTACAGCTCCATCGACAC
  TGTGAAGAACCTCTGGGAGCTAGAGCTTGTCAATGTCCTGAAAGTTCACCTGCTGGGCCACCGCAAGCGC
  ATTATCGCCTCTCTAGCAGACAGGCCCTATGAGGAGCCACCCCAGAAGCCTCCAAGGTTTTCCCAGCTAA
  GATGCCAGGATTTAATCTCCCAGACATCGTCCCCGCTGAGCCAGAATGATTCCTGCACAGGGCGGTCGGC
  AGACCTGCTGCTGCCCTCAGCAGACACAAGCAGGAGGCGGCACGACAGCCTGCCCGACCCTGGGACCGCC
  TCCCGAGCAGACCGCTTCCGAGTCCAGGAGGAGCCCAGTGAGACCAAGCTGACCCTCCGTCCACCCAGCC
  TAGCCGCGCCCTATGCTCCTGTTCAGAGCTGGCAGCACCAGCCAGAAAAGCTCATCTTTGAGTCCTGCGG
  TTATGAGGCCAATTATCTGGGTTCCATGCTGATCAAAGATCTGCGAGGGACAGAGTCCACCCAAGATGCC
  TGTGCCAAAATGCGGAAGTCAACGGAGCACATGAAGAAGATCCCCACCATCATCCTGTCCATCACGTACA
  AAGGTGTCAAGTTCATCGATGCTTCCAACAAGAACGTCATTGCGGAGCACGAGATCCGGAACATTTCCTG
  CGCCGCGCAGGACCCAGAGGACCTGTGTACCTTCGCGTACATCACCAAGGACCTGCAGACGAGCCACCAC
  TACTGCCACGTTTTCAGCACAGTGGATGTGAACCTGACCTACGAGATCATCCTGACCCTGGGGCAGGCAT
  TTGAGGTGGCCTATCAGCTGGCCCTCCAAGCCCAGAAATCCAGGACCATGGCGGCCTCTGCAGCTTCGAT
  GATTGAGACAAAATCCTCCAAACCAGTGCCTAAGCCTCGGGTCGGCATGAGGAAGTCTGCAGTGCCGGTG
  CCCCCCGACTCTCGCTGTTGTCACTGTCACACCTGCACCACTCACCGTCCTTCCTACCTACCGCTGCCGT
  CCGTTAGTCCTGGAGTCAAGCTGGAACCACCTGATTCTGACCAAGAGGCCCCGTCCCACGCTAGTGTTTC
  CTGGATCGTGGACCCAAAGCCAGACTCGAAGCGGAGCCTCAGCACCAAGTACGAGACCACTATCTTCTAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001286040
• Insert Size: 3570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286040.1, NP_001272969.1
• RefSeq Size: 7092 bp
• RefSeq ORF: 3570 bp
• Locus ID: 224650
• Cytogenetics: 17 A3.3
• Gene Summary: Regulator of different signaling pathways. Regulates EPHA8 receptor tyrosine kinase signaling to control cell migration and neurite retraction.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.