Spag9 (NM_001199205) Mouse Untagged Clone

CAT#: MC229445

Spag9 (untagged) - Mouse sperm associated antigen 9 (Spag9), transcript variant 7

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Spag9
• Synonyms: 3110018C07Rik; 4733401I23Rik; 4831406C20Rik; AW552012; Jip4; JLP; JSAP2; JSAP2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229445 representing NM_001199205
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCTGGATGCATGCTGCTGTTTGTGTTTGGCTTTGTTGGCGGGGCGGTGGTCATTAATTCTGCGA
  TCTTAGTATCTCTCTCTGTTTTGCTGCTTGTGCACTTTTCTATTTCTACCGGGGTGCCAGCGCTGACGCA
  GAACCTACCAAGGATACTCAGAAAAGAACGTCCTATATCATTAGGGATTTTCCCTCTACCTGCTGGAGAT
  GGATTGCTTACACCTGACACTCAGAAAGGAGGCGAGACCCCAGGATCAGAGCAATGGAAATTTCAAGAAT
  TAAGTCAACCACGTTCTCATACCAGCCTGAAGGATGAACTTTCTGATATTAGTCAAGGTGGATCTAAAGC
  TACCACTCCAGCTTCAACAGCAAATTCAGACGTATCAGCAATTCCTCCTGATACTCCGTCAAAGGAAGAT
  AATGAAGGATTTGTAAAAGGCACAGATACATCAAATAAGTCAGAGATAAGCAAACACATAGAAGTCCAGG
  TTGCCCAAGAGACTAGGAATGTGTCTACAGAATCTGGTGAAAATGAAGAGAAATCAGAAGTTCAAGCAAT
  CATTGAATCCACTCCTGAATTGGATATGGACAAAGACCTCAGTGGATATAAAGGTTCAAGCACTCCCACC
  AAAGGCATAGAGAACAAAGCTTTTGATCGCAATACAGAATCTCTCTTTGAAGAGCTGTCCTCAGCTGGCT
  CGGGCCTTATTGGAGATGTGGATGAAGGAGCGGATTTACTAGGAATGGGTCGTGAGGTGGAGAATCTCAT
  ACTGGAGAACACACAGCTGCTGGAAACCAAAAACGCTCTGAATGTAGTGAAGAATGACTTAATAGCCAAA
  GTGGATGAGCTGACCTGTGAGAAGGACGTGCTGCAGGGGGAGCTGGAGGCTGTGAAGCAAGCCAAGCTGA
  AGCTGGAGGACAAGAACAGAGAGCTGGAGGAGGAGCTCAGGAAAGCTCGTGCAGAAGCTGAAGATGCAAG
  GCAAAAAGCAAAAGACGATGATGATAGTGACATTCCCACAGCCCAGAGGAAGAGGTTTACCAGAGTAGAA
  ATGGCCCGAGTTCTCATGGAGAGAAACCAGTACAAAGAGAGGCTGATGGAGCTTCAAGAAGCTGTTCGAT
  GGACGGAGATGATCCGGGCCTCACGAGAGAACCCAGCTATGCAAGAAAAGAAAAGGTCAAGCATCTGGCA
  ATTTTTCAGCCGACTTTTTAGCTCTTCAAGTAATGCAACAAAGAAGCCTGAACCCCCTGTTAACCTGAAG
  TACAACGCCCCCACCTCTCACGTGACTCCCTCCGTGAAGAAAAGGAGCAGCACTTTATCTCAGCTCCCTG
  GGGACAAGTCCAAGGCATTTGATTTCCTCAGTGAGGAAACTGAGGCTAGTTTAGCCTCCCGCAGAGAGCA
  AAAGAGAGAGCAGTATCGGCAGGTGAAGGCTCACGTTCAGAAGGAAGATGGCCGAGTGCAAGCTTTCGGC
  TGGAGTCTGCCTCAGAAGTACAAACAGGTAGCCAATGGTCAAGGGGAAACTAAGATGAAAAATTTACCTG
  TGCCTGTCTATCTCAGACCACTAGACGAAAAAGATGCGTCTATGAAGCTGTGGTGTGCTGTAGGAGTCAA
  TTTATCTGGTGGCAAGACAAGAGATGGCGGGTCTGTTGTTGGAGCGAGTGTATTTTACAAGGATATTGCT
  GGTTTGGACACTGAAGGCAGTAAACAGCGGAGTGCGTCTCAGAGTAGTTTAGACAAGTTGGATCAAGAAC
  TCAAGGAACAGCAGAAAGAATTTAAAAATCAAGAAGAGTTGTCCAGTCAGGTCTGGATCTGTACCAGCAC
  CCACTCAACTACAAAGGTTATCATCATTGATGCTGTTCAGCCTGGCAACATCCTAGATAGTTTCACTGTT
  TGCAATTCTCATGTTCTGTGCATTGCCAGTGTCCCAGGAGCTCGGGAAACAGACTACCCTGCAGGAGAAG
  AACTTTCTGAATCTGGTCAGGTAGACAAAGCGTCATTGTGTGGAAGCATGACCAGTAACAGCTCCGCAGA
  GATGGACAGCTTGCTGGGAGGCATCACAGTGGTTGGGTGCTCCACAGAAGGACTGACAGGAGCTGCCACT
  TCCCCAAGTACCAACGGTGCTTCTCCTGTGATTGAAAAGCCACCAGAAATGGAAACTGAAAATAGCGAAG
  TTGATGAAAATATTCCAACAGCAGAAGAAGCAACTGAAGCCACAGAGGGCAATGCAGGGTCCACTGAAGA
  CACTGTGGACATCTCCCAGCCTGGCGTGTACACAGAGCATGTGTTTACAGATCCACTGGGAGTTCAGATC
  CCAGAAGACCTCTCCCCAGTGTTTCAGTCAAGTAATGACTCAGATGTGTACAAAGATCAAATATCAGTAT
  TGCCAAATGAACAAGACCTTGCGAGAGAAGAAGCTCAGAAAATGAGTAGTCTTTTACCAACCATGTGGCT
  TGGAGCTCAGAATGGCTGTTTGTATGTCCACTCATCTGTAGCCCAGTGGAGGAAATGTCTCCATTCCATT
  AAGCTCAAAGACTCAATTCTCAGTATTGTACATGTGAAAGGAATTGTACTAGTGGCCCTGGCGGATGGCA
  CCCTTGCAATCTTCCACAGAGGAGTTGATGGACAATGGGATCTGTCAAACTATCACCTTTTAGACCTTGG
  ACGTCCTCATCATTCCATACGATGCATGACTGTGGTACATGACAAAGTCTGGTGTGGCTATAGGAACAAA
  ATCTATGTGGTTCAACCAAAGGCTATGAAGATAGAGAAATCATTTGATGCACACCCCAGGAAGGAGAGCC
  AAGTACGGCAGCTTGCATGGGTGGGTGATGGTGTATGGGTCTCCATTCGTTTGGATTCCACGCTCCGCCT
  CTATCATGCACACACATACCAACATCTCCAGGATGTGGATATTGAGCCTTATGTAAGCAAAATGTTAGGT
  ACTGGAAAACTGGGTTTCTCTTTCGTGAGGATAACAGCTCTTATGGTGTCTTGCAATCGTTTGTGGGTGG
  GGACTGGAAATGGTGTCATTATCTCCATCCCACTGACAGAAACCGTAATCCTCCACCAGGGACGTTTACT
  GGGGCTAAGGGCAAATAAAACCTCAGGGACTCCAGGTAACCGTCCTGGAAGTGTAATCCGTGTATATGGT
  GATGAGAACAGTGATAAAGTGACTCCAGGGACATTTATACCCTACTGTTCAATGGCACATGCACAGCTTT
  GTTTCCACGGGCACCGGGATGCTGTGAAATTCTTTGTGGCAGTCCCAGGTCAGGTCATTAGCCCACAAAG
  TAGCAGTGGTGGGGCAGATCTTACAGCTGACAAGGCAGGGTCATCTGCACAGGAGCCCAGTAGCCAGACG
  CCCTTGAAGTCCATGCTTGTCATCAGTGGAGGAGAGGGCTACATTGACTTCCGGATGGGTGATGAAGGTG
  GAGAATCTGAACTTCTGGGAGAGGATCTTCCACTGGAACCTTCAGTCACCAAAGCCGAGAGGAGTCACCT
  GATAGTGTGGCAAGTGATGTGTGGCAATGAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199205
• Insert Size: 3534 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199205.1, NP_001186134.1
• RefSeq Size: 6940 bp
• RefSeq ORF: 3534 bp
• Locus ID: 70834
• UniProt ID: Q58A65
• Cytogenetics: 11 D
• Gene Summary: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module (PubMed:12391307, PubMed:15767678). Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (7) has a different 5' terminus and has multiple differences in the coding region, compared to variant 5, which results in a shorter isoform (7) with a different N-terminus, compared to isoform 5.