Atp8a1 (NM_001284345) Mouse Untagged Clone

CAT#: MC229428

Atp8a1 (untagged) - Mouse ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1 (Atp8a1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atp8a1
• Synonyms: AI481521; AI853962; APLT; Atp3a2; AW743152; AW822227; B230107D19Rik; ClassI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229428 representing NM_001284345
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGACCATGCGGAGGACAGTGTCGGAGATCCGCTCGCGCGCGGAAGGTTATGAGAAGACAGATGATG
  TTTCAGAGAAGACCTCGCTGGCAGATCAGGAGGAAGTGAGAACCATCTTCATCAACCAGCCTCAGCTGAC
  AAAATTCTGCAACAACCACGTCAGTACTGCAAAATACAACGTGATCACATTCCTTCCGAGGTTCCTCTAT
  TCTCAGTTCCGAAGAGCGGCTAATTCGTTCTTTCTCTTTATTGCCCTGCTCCAGCAAATTCCTGATGTGT
  CGCCGACCGGTCGTTACACAACACTGGTTCCTCTCTTATTTATTTTAGCTGTGGCTGCTATTAAAGAGAT
  AATAGAAGATATTAAACGACACAAGGCTGATAATGCTGTGAACAAGAAACAGACACAAGTTTTGAGAAAT
  GGTGCATGGGAAATTGTTCACTGGGAAAAGGTAAATGTTGGAGATATAGTTATAATAAAAGGCAAAGAGT
  ATATACCTGCTGACACTGTCCTTCTCTCGTCAAGTGAGCCCCAGGCCATGTGCTACATCGAGACATCCAA
  CTTAGATGGTGAAACAAACTTGAAAATTAGACAAGGCTTACCGGCAACATCGGATATCAAAGACATTGAC
  AGTTTGATGAGAATTTCTGGCAGAATCGAGTGTGAAAGTCCAAACCGACATCTCTACGATTTTGTTGGGA
  ACATAAGGCTTGATGGCCATGGCACCGTTCCCCTGGGGGCAGACCAGATCCTTCTCCGAGGAGCTCAGTT
  AAGAAATACCCAGTGGGTTCATGGGATAGTTGTCTACACTGGCCACGACACCAAGCTGATGCAGAATTCC
  ACAAGCCCACCACTTAAACTCTCCAATGTGGAACGGATTACAAATGTACAAATTCTGATTTTATTTTGCA
  TCTTAATTGCCATGTCTCTTGTGTGTTCTGTGGGCTCAGCCATTTGGAACCGAAGGCATTCCGGGAAAGA
  CTGGTACCTCCATCTACACTATGGTGGCGCTAGTAACTTTGGACTGAACTTCCTGACTTTCATTATCCTT
  TTCAACAACCTCATTCCCATCAGCTTGCTGGTCACATTAGAAGTGGTGAAGTTTACTCAGGCATACTTCA
  TAAATTGGGATCTTGACATGCATTATGAGCCCACAGACACCGCAGCAATGGCTCGGACATCTAATCTGAA
  TGAGGAACTTGGCCAGGTTAAATACATATTTTCTGACAAAACTGGGACCCTGACATGCAATGTGATGCAG
  TTCAAGAAGTGCACCATCGCGGGCGTGGCCTATGGCCATGTCCCTGAACCTGAGGATTATGGGTGCTCGC
  CCGATGAATGGCAGAGCTCACAGTTTGGAGATGAAAAAACCTTTAATGACCCGTCGTTGCTGGACAATCT
  CCAGAATAACCACCCAACCGCACCTATCATCTGTGAATTTCTCACAATGATGGCCGTCTGCCACACAGCT
  GTACCGGAGAGAGAAGGGGACAAGATCATTTATCAGGCTGCATCGCCAGATGAGGGTGCACTGGTCAGAG
  CCGCCAAGCAGTTGAATTTTGTCTTCACTGGAAGAACTCCTGACTCTGTCATCATAGATTCACTGGGGCA
  GGAAGAAAGATATGAATTGCTCAATGTCCTAGAGTTCACCAGTGCTAGGAAGAGGATGTCGGTGGTGGTT
  CGCACTCCATCCGGGAAGTTACGGCTCTACTGCAAAGGAGCTGACACAGTAATTTATGAACGACTTGCAG
  AGACCTCAAAGTACAAAGAAATCACCCTAAAACACTTGGAACAGTTTGCTACAGAAGGGCTGAGGACTTT
  GTGTTTTGCTGTGGCTGAGATTTCTGAGAGCGACTTCGAGGAGTGGCGGGCCGTCTACCACCGCGCATCC
  ACGTCGGTACAGAACAGGCTGCTGAAGCTGGAGGAGAGCTACGAACTAATTGAAAAGAATCTTCAGCTAC
  TTGGAGCTACAGCCATTGAGGATAAATTGCAGGACCAAGTGCCTGAAACCATAGAAACGCTAATGAAGGC
  TGACATCAAAATATGGATCCTTACTGGGGACAAGCAAGAAACTGCCATTAATATTGGACACTCCTGTAGA
  CTCCTCAAGAGGAACATGGGAATGATCGTGATAAACGAGGGCTCTCTTGACGGCACGAGGGAAACTCTCA
  GCCGCCACTGCACCACCCTGGGAGATGCTCTTCGGAAGGAAAATGATTTTGCTCTTATAATTGATGGGAA
  GACCCTCAAATATGCCTTAACCTTTGGCGTCCGGCAGTATTTCCTGGACTTAGCTCTGTCCTGCAAAGCT
  GTCATTTGCTGCAGGGTTTCTCCTCTTCAGAAGTCCGAGGTTGTCGAGATGGTTAAGAAACAAGTCAAAG
  TCATCACACTTGCCATCGGGGATGGAGCAAATGACGTCAGCATGATCCAGACGGCCCATGTGGGTGTTGG
  GATAAGCGGCAATGAAGGTTTGCAGGCAGCCAACTCTTCAGATTACTCCATCGCTCAGTTCAAATATTTG
  AAGAATTTGTTGATGGTTCACGGTGCCTGGAATTACAATAGAGTCTCCAAGTGTATCCTGTACTGCTTCT
  ACAAGAACATCGTGCTCTACATCATCGAGATCTGGTTTGCCTTTGTCAACGGCTTTTCTGGACAGATCCT
  CTTTGAAAGATGGTGTATAGGGCTTTATAATGTGATGTTCACAGCGATGCCTCCCTTGACACTTGGAATA
  TTTGAGAGATCGTGCAGAAAGGAGAACATGTTGAAGTATCCTGAGTTGTACAAAACATCCCAGAATGCTC
  TGGACTTCAATACCAAGGTTTTCTGGGTTCATTGTTTGAATGGCCTCTTCCACTCCGTTATTCTGTTTTG
  GTTCCCACTGAAAGCCCTGCAGTATGGCACGGTATTTGGAAATGGGAAAACCTCAGATTACCTGCTTCTG
  GGAAACTTTGTTTATACTTTTGTAGTGATAACTGTGTGCTTGAAAGCTGGACTGGAAACCTCCTATTGGA
  CATGGTTCAGCCACATCGCCATCTGGGGCAGCATCGCGCTCTGGGTGGTGTTCTTCGGGATCTACTCATC
  TCTGTGGCCTGCCGTGCCCATGGCCCCTGACATGTCCGGAGAGGCAGCCATGCTCTTCAGCTCTGGAGTC
  TTCTGGGTGGGCTTGCTCTCCATCCCTGTGGCGTCCTTGCTTCTGGATGTGCTGTACAAAGTCATCAAGA
  GGACGGCCTTTAAAACCTTAGTGGATGAAGTTCAGGAGCTTGAGGCGAAATCTCAAGACCCGGGCGCAGT
  CGTACTTGGAAAGAGCCTCACGGAGAGAGCGCAGCTGCTCAAGAACGTCTTTAAGAAGAACCACGTGAAT
  CTGTACCGCTCCGAGTCCCTGCAGCAGAACCTGCTTCACGGCTATGCTTTCTCTCAAGATGAAAACGGCA
  TCGTCTCACAGTCTGAAGTCATCAGAGCCTATGACACCACGAAACAGAGGCCCGATGAGTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001284345
• Insert Size: 3495 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284345.1, NP_001271274.1
• RefSeq Size: 8176 bp
• RefSeq ORF: 3495 bp
• Locus ID: 11980
• UniProt ID: P70704
• Cytogenetics: 5 C3.1
• Gene Summary: Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. In vitro, its ATPase activity is selectively and stereospecifically stimulated by phosphatidylserine (PS). The flippase complex ATP8A1:TMEM30A seems to play a role in regulation of cell migration probably involving flippase-mediated translocation of phosphatidylethanolamine (PE) at the plasma membrane. Acts as aminophospholipid translocase at the plasma membrane in neuronal cells; the activity is associated with hippocampus-dependent learning.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate exon and contains a different alternate exon in the 5' coding region, compared to variant 1. It encodes isoform c, which is of the same size but lacks an internal segment, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.