Tek (NM_001290549) Mouse Untagged Clone

CAT#: MC229389

Tek (untagged) - Mouse endothelial-specific receptor tyrosine kinase (Tek), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tek
• Synonyms: AA517024; Cd202b; Hyk; STK1; Tie-2; Tie2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229389 representing NM_001290549
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCTTTAGCCGGCTTAGTTCTCTGTGGAGTCAGCTTGCTCCTTTATGGAGTAGTAGAAGGCGCCA
  TGGACCTGATCTTGATCAATTCCCTACCTCTTGTGTCTGATGCCGAAACATCCCTCACCTGCATTGCCTC
  TGGGTGGCACCCCCATGAGCCCATCACCATAGGAAGGGACTTTGAAGCCTTAATGAACCAGCACCAAGAT
  CCACTGGAGGTTACTCAAGATGTGACCAGAGAATGGGCGAAAAAAGTTGTTTGGAAGAGAGAAAAGGCCA
  GTAAGATTAATGGTGCTTATTTCTGTGAAGGTCGAGTTCGAGGACAGGCTATAAGGATACGGACCATGAA
  GATGCGTCAACAAGCATCCTTCCTACCTGCTACTTTAACTATGACCGTGGACAGGGGAGATAATGTGAAC
  ATATCTTTCAAAAAGGTGTTAATTAAAGAAGAAGATGCAGTGATTTACAAAAATGGCTCCTTCATCCACT
  CAGTGCCCCGGCATGAAGTACCTGATATTTTAGAAGTTCACTTGCCGCATGCTCAGCCCCAGGATGCTGG
  TGTGTACTCGGCCAGGTACATAGGAGGAAACCTGTTCACCTCAGCCTTCACCAGGCTGATTGTTCGGAGA
  TGTGAAGCTCAGAAGTGGGGGCCCGACTGTAGCCGTCCTTGTACTACTTGCAAGAACAATGGAGTCTGCC
  ATGAAGATACCGGGGAATGCATTTGCCCTCCTGGGTTTATGGGGAGAACATGTGAGAAAGCTTGTGAGCC
  GCACACATTTGGCAGGACCTGTAAAGAAAGGTGTAGTGGACCAGAAGGATGCAAGTCTTATGTGTTCTGT
  CTCCCAGACCCTTACGGGTGTTCCTGTGCCACAGGCTGGAGGGGGTTGCAGTGCAATGAAGCATGCCCAT
  CTGGTTACTACGGACCAGACTGTAAGCTCAGGTGCCACTGTACCAATGAAGAGATATGTGATCGGTTCCA
  AGGATGCCTCTGCTCTCAAGGATGGCAAGGGCTGCAGTGTGAGAAAGAAGGCAGGCCAAGGATGACTCCA
  CAGATAGAGGATTTGCCAGATCACATTGAAGTAAACAGTGGAAAATTTAACCCCATCTGCAAAGCCTCTG
  GGTGGCCACTACCTACTAGTGAAGAAATGACCCTAGTGAAGCCAGATGGGACAGTGCTCCAACCAAATGA
  CTTCAACTATACAGATCGTTTCTCAGTGGCCATATTCACTGTCAACCGAGTCTTACCTCCTGACTCAGGA
  GTCTGGGTCTGCAGTGTGAACACAGTGGCTGGGATGGTGGAAAAGCCTTTCAACATTTCCGTCAAAGTTC
  TTCCAGAGCCCCTGCACGCCCCAAATGTGATTGACACTGGACATAACTTTGCTATCATCAATATCAGCTC
  TGAGCCTTACTTTGGGGATGGACCCATCAAATCCAAGAAGCTTTTCTATAAACCTGTCAATCAGGCCTGG
  AAATACATTGAAGTGACGAATGAGATTTTCACTCTCAACTACTTGGAGCCGCGGACTGACTACGAGCTGT
  GTGTGCAGCTGGCCCGTCCTGGAGAGGGTGGAGAAGGGCATCCTGGGCCTGTGAGACGATTTACAACAGC
  GTCTATCGGACTCCCTCCTCCAAGAGGTCTCAGTCTCCTGCCAAAAAGCCAGACAGCTCTAAATTTGACT
  TGGCAACCGATATTTACAAACTCAGAAGATGAATTTTATGTGGAAGTCGAGAGGCGATCCCTGCAAACAA
  CAAGTGATCAGCAGAACATCAAAGTGCCTGGGAACCTGACCTCGGTGCTACTGAGCAACTTAGTCCCCAG
  GGAGCAGTACACAGTCCGAGCTAGAGTCAACACCAAGGCGCAGGGGGAGTGGAGTGAAGAACTCAGGGCC
  TGGACCCTTAGTGACATTCTCCCTCCTCAACCAGAAAACATCAAGATCTCCAACATCACTGACTCCACAG
  CTATGGTTTCTTGGACAATAGTGGATGGCTATTCGATTTCTTCCATCATCATCCGGTATAAGGTTCAGGG
  CAAAAATGAAGACCAGCACATTGATGTGAAGATCAAGAATGCTACCGTTACTCAGTACCAGCTCAAGGGC
  CTAGAGCCAGAGACTACATACCATGTGGATATTTTTGCTGAGAACAACATAGGATCAAGCAACCCAGCCT
  TTTCTCATGAACTGAGGACGCTTCCACATTCCCCAGCCTCTGCAGACCTCGGAGGGGGAAAGATGCTACT
  CATAGCCATCCTTGGGTCGGCTGGAATGACTTGCATCACCGTGCTGTTGGCGTTTCTGATTATGTTGCAA
  CTGAAGAGAGCAAATGTCCAAAGGAGAATGGCTCAGGCATTCCAGAACAGAGAAGAACCAGCTGTGCAGT
  TTAACTCAGGAACTCTGGCCCTTAACAGGAAGGCCAAAAACAATCCGGATCCCACAATTTATCCTGTGCT
  TGACTGGAATGACATCAAGTTTCAAGACGTGATCGGAGAGGGCAACTTTGGCCAGGTTCTGAAGGCACGC
  ATCAAGAAGGATGGGTTACGGATGGATGCCGCCATCAAGAGGATGAAAGAGTATGCCTCCAAAGATGATC
  ACAGGGACTTCGCAGGAGAACTGGAGGTTCTTTGTAAACTTGGACACCATCCAAACATCATTAATCTCTT
  GGGAGCATGTGAACACCGAGGCTATTTGTACCTAGCTATTGAGTATGCCCCGCATGGAAACCTCCTGGAC
  TTCCTGCGTAAGAGCAGAGTGCTAGAGACAGACCCTGCTTTTGCCATCGCCAACAGTACAGCTTCCACAC
  TGTCCTCCCAACAGCTTCTTCATTTTGCTGCAGATGTGGCCCGGGGGATGGACTACTTGAGCCAGAAACA
  GTTTATCCACAGGGACCTGGCTGCCAGAAACATTTTAGTTGGTGAAAACTACATAGCCAAAATAGCAGAT
  TTTGGATTGTCACGAGGTCAAGAAGTGTATGTGAAAAAGACAATGGGAAGGCTCCCAGTGCGTTGGATGG
  CAATCGAATCACTGAACTATAGTGTCTATACAACCAACAGTGATGTCTGGTCCTATGGTGTATTGCTCTG
  GGAGATTGTTAGCTTAGGAGGCACCCCCTACTGCGGCATGACGTGCGCGGAGCTCTATGAGAAGCTACCC
  CAGGGCTACAGGCTGGAGAAGCCCCTGAACTGTGATGATGAGGTGTATGATCTAATGAGACAGTGCTGGA
  GGGAGAAGCCTTATGAGAGACCATCATTTGCCCAGATATTGGTGTCCTTAAACAGGATGCTGGAAGAACG
  GAAGACATACGTGAACACCACACTGTATGAGAAGTTTACCTATGCAGGAATTGACTGCTCTGCGGAAGAA
  GCAGCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290549
• Insert Size: 3369 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290549.1, NP_001277478.1
• RefSeq Size: 4699 bp
• RefSeq ORF: 3369 bp
• Locus ID: 21687
• UniProt ID: Q02858
• Cytogenetics: 4 43.34 cM
• Gene Summary: Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1, SHC1 and TIE1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. It encodes isoform 2, which is shorter by an amino acid, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.