Adamts2 (NM_001277305) Mouse Untagged Clone

CAT#: MC229366

Adamts2 (untagged) - Mouse a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 2 (Adamts2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Adamts2
• Synonyms: ADAM-TS2; ADAMTS-2; ADAMTS-3; hPCPNI; mKIAA4060; PCINP; pNPI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229366 representing NM_001277305
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCCGCCGGCGGGAGCCGCTCGCCGCCTGCTCTGCCCCGCTCTACTGCTGCTGCTGCTGCCGCCGC
  CGCCTCTCCTGCTGCTGCCGCCGCCGCCCGCGAGCGTCCGGCTCGTCGCCGCCACCGAGCCCCCAGGCGG
  GCCCCCGGGGCAAGGAGCCGAGCGCATCCTGGCGGTGCCGGTGCGCACTGACGCCCAGGGCCGCTTGGTG
  TCCCACGTGGTGTCTTTGGAGACGGCCGGGGCGGGGGTACGAGCCCGCAGAGCTGCCCTGGACCAGACCT
  CGGGCTTGCCCGGAGGCGCTGCTCAGGACCCTGGAGGACGCCTTTTCTACAACCTCACAGTATTCGGCCG
  AGACCTGCACCTGCGGCTGCGGCCCAACGCCCGGCTCGTGGCTCCGGGGGCCACCGTGGAGTGGCAGGGT
  GAGACGGGTGACACCCGAGTGGAGCCCCTGCTTGGGAGCTGTCTCTACGTTGGAGACGTGGCAGACTTAC
  CTAAAGCCTCCTCGGTGGCGCTCAGTAACTGTGATGGGCTGGCTGGTCTGATCCGGATGGAGGAGGAGGA
  GTTCTTTATCGAACCTCTGGAGAAGGGGCAGACGGATCAGGAGGCTGAACAAGGCCGAGTGCATGTGGTA
  TATCGCCGACCGCCCACTCCCAAGCCCCCTCCTGTGAGCGAACCACAGGCTCTGGACACAGGAGTCTCCC
  AGGGCAACCTGGACAGCCTCAGCAGAGCCCTAGGCGTCCTGGAGGAGCGCATCAACAGCTCACGGCGGAG
  GGTGCGCAGGCATGCCACGGACGATGACTACAACATTGAGGTCCTGCTGGGTGTGGATGACTCGGTGGTC
  CAGTTCCATGGGAAGGAGCACGTGCAGAAGTACCTGCTGACACTCATGAACATTGTCAACGAAATCTACC
  ATGATGAGTCGTTGGGGGCCCACATCAATGTGGTGCTGGTGAGGATTATCCTGCTGAGCCACGCAAAGTC
  CATGAGCCTCATTGAGATTGGAAACCCCTCTCAGAGTCTGGAGAATGTCTGCCGATGGGCCTACCTCCAG
  CAGAAGCCGGACACAGACCACGATGAGTACCACGACCATGCCATCTTTCTCACACGGCAGGATTTTGGGC
  CCTCGGGCATGCAAGGCTATGCTCCCGTCACCGGGATGTGCCACCCTGTCCGCAGCTGTACTCTGAACCA
  TGAAGATGGCTTCTCCTCGGCATTTGTGGTGGCTCACGAGACGGGCCATGTGCTGGGCATGGAGCATGAC
  GGGCAAGGCAACCGCTGCGGTGACGAGGTGCGGCTGGGCAGCATCATGGCCCCCCTGGTACAGGCTGCCT
  TCCATCGCTTCCACTGGTCCCGCTGCAGCCAGCAGGAGCTCAGCCGCTACCTGCATTCCTATGACTGCCT
  GCGGGATGATCCCTTCGCCCACGACTGGCCAGCATTGCCTCAACTCCCAGGGCTTCACTATTCCATGAAC
  GAGCAGTGTCGCTTTGACTTTGGCCTGGGTTACATGATGTGCACTGCGTTCCGGACCTTCGACCCCTGCA
  AACAGCTCTGGTGCAGCCACCCAGACAACCCCTACTTTTGCAAGACAAAGAAGGGTCCTCCACTGGATGG
  TACTATGTGTGCGCCTGGCAAGCACTGCTTCAAAGGCCACTGCATCTGGCTGACACCCGATATCCTCAAA
  CGAGACGGCAACTGGGGTGCCTGGACTCCGTTTGGCTCCTGCTCACGCACCTGTGGCACAGGCGTGAAGT
  TCAGGACCCGCCAGTGTGACAACCCACATCCAGCCAATGGGGGCCGCACCTGCTCGGGCCTGGCCTATGA
  CTTCCAGCTCTGCAACCCCCAGGACTGTCCCAATTCCCTGGCTGACTTCCGGGAGGAGCAGTGTCAGCAG
  TGGGACCTGTACTTTGAGCACGGTGATGTCCAGCACCACTGGCTGCCTCATGAGCACCGGGATGCCAAGG
  AGAGATGCCACCTCTACTGTGAGTCCAAGGAGACAGGGGAGGTGGTGTCTATGAAGCGCATGGTGCACGA
  TGGAACACGCTGCTCCTACAAGGACGCCTTCAGCCTGTGTGTGCGTGGGGACTGCAGGAAAGTGGGATGT
  GATGGGGTGATCGGGTCCCGTAAGCAGGAAGACAAGTGTGGCGTGTGTGGAGGCGACAACACCCACTGTA
  AAGTGGTGAAGGGCACATTCACGAGGTCACCGAGGAAACAAGATTACATCAAGATGTTTGAGATCCCCGC
  AGGAGCCAGACACCTGCTCATCCAGGAGGCCGACACCACCAGCCACCATCTGTCTGTCAAGAACCTGGAG
  ACAGGCAAGTTCATCTTAAATGAAGAGAACCACTTGGACCCCAATTCTAGATCCTTCATTGCCATGGGTG
  TGGAGTGGGAATACAGGAACGAGGACGAGAGGGAGACGCTGCAGACCATAGGCCCACTCCACGGAACCAT
  CACCGTGCTGGTTATTCCTGAAGGAGACACCAGGATCTCACTGACATACAAATACATGATTCACGAGGAC
  TCCCTCAATGTTGACGACAACAATGTCCTGGAAGATGATGCTGTGCGCCACGAGTGGGCCCTGAAGAAGT
  GGTCTCCCTGCTCCAAGCCCTGCGGTGGAGGGTCCCAGTTCACTAAGTATGGCTGCCGCCGGAGGTTGGA
  CAGCAAGATGGTACACCGAGCCTTCTGCAGTGCCCTCGCCAAGCCCAAGGCCATCCGACGAGCCTGCAAC
  CCTCAGGAGTGCTCCCAGCCTGTGTGGGTCACCGGTGAATGGGAGCCTTGTACTCAAAGCTGTGGGCGAA
  CTGGCATGCAGGTGCGCTCCGTCCGCTGTATACAACCTCTGCACAACAACACCACCCGCTCCGTGCACAC
  CAAACACTGCAACGATCACCGTCCCGAGAGCCGCCGGGCCTGCAACCGTGAGCTCTGCCCTGGGCGGTGG
  CGGGCTGGGTCCTGGTCCCAGTGTTCGGTAACCTGTGGCAACGGCACCCAGGAACGGCCAGTGCTCTGTC
  GCACTGCAGATGATAATTTTGGTGTCTGCCGGGAGGAGCGACCGGAGACGGCAAGGATCTGCAGGCTTGC
  ACCCTGTCCCCGAAACGGCTCAGATCCCTCCAAGAAGAGCTACGTGGTTCAGTGGCTGTCCCGGCCTGAT
  CCTGACTCACCTATCCAGAAGATCTCGTCAAGTAACCGACCCGTTTATAACTCTGCCTCTGCCCTCCAGT
  GCATGCCCTGCGCCTTGTGGAGCTTGCCTTGGGCTCCCCTCCTCTTTCTCTATGGAAAGCTTCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001277305
• Insert Size: 3288 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277305.1, NP_001264234.1
• RefSeq Size: 5094 bp
• RefSeq ORF: 3288 bp
• Locus ID: 216725
• Cytogenetics: 11 B1.3
• Gene Summary: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin repeats) family of proteinases that is involved in the proteolytic processing of procollagens. The encoded protein precursor is proteolytically processed to generate a mature, zinc-dependent enzyme. Mice lacking the encoded protein develop abnormal lungs, fragile skin and male sterility. [provided by RefSeq, Aug 2015]