Cp (NM_001276248) Mouse Untagged Clone

CAT#: MC229349

Cp (untagged) - Mouse ceruloplasmin (Cp), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cp
• Synonyms: D3Ertd555; D3Ertd555e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229349 representing NM_001276248
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTTTTTGCTGCTTAGCACATTTATATTTTTGTATAGTTCCTTAGCCTTGGCAAGAGATAAGCATT
  ATTTCATTGGAATTACTGAAGCAGTCTGGGACTATGCTTCTGGCACTGAAGAAAAGAAACTTATTTCAGT
  TGACACGGAACAGTCCAATTTCTATCTTCAAAATGGTCCAGATCGTATTGGAAGAAAATATAAGAAGGCC
  CTTTATTTTGAGTACACAGATGGCACCTTTAGTAAGACTATAGACAAACCAGCCTGGCTAGGGTTTTTAG
  GCCCTGTCATCAAAGCTGAAGTTGAAGATAAAGTTTATGTTCACTTAAAGAACCTTGCCTCTAGGATCTA
  CACTTTTCATGCACATGGGGTAACGTACACCAAGGAGTATGAGGGAGCCGTCTACCCTGACAACACCACT
  GATTTTCAACGGGCTGATGACAAAGTGCTTCCCGGACAACAGTATGTGTATGTGCTGCATGCCAATGAGC
  CAAGTCCTGGAGAGGGAGACAGCAATTGTGTGACCAGGATTTACCACTCCCATGTTGATGCTCCAAAAGA
  TATTGCATCAGGACTCATAGGACCTCTAATACTCTGTAAAAAAGGTTCTCTATATAAGGAAAAAGAGAAA
  AATATTGACCAAGAATTTGTACTAATGTTCTCTGTGGTGGATGAAAATCTCAGCTGGTATCTGGAAGATA
  ACATCAAAACCTTCTGCTCTGAACCCGAGAAAGTTGATAAAGACAATGAAGACTTCCAGGAAAGCAACAG
  GATGTACTCTATAAATGGATATACATTTGGAAGCCTCCCAGGGCTCTCGATGTGTGCAGCAGACAGAGTG
  AAGTGGTACCTTTTTGGTATGGGTAATGAAGTTGATGTGCATTCAGCTTTCTTTCATGGCCAAGCCCTGA
  CCAGCAGGAACTATCAAACCGATATAATCAACCTGTTCCCTGCCACCCTAATTGATGCTTATATGGTGGC
  CCAGAATCCTGGAGTCTGGATGCTCAGTTGCCAGAACCTAAACCATCTGAAAGCTGGGTTGCAGGCCTTT
  TTCCAGGTTCGTGACTGTAACAAGCCCTCGCCAGAGGATAATATCCAAGATAGGCATGTGAGACACTATT
  ACATTGCTGCTGAGGAGGTCATCTGGAATTATGCTCCTTCTGGGACGGACATCTTCACTGGAGAGAATTT
  AACAGCTCTGGAAAGTGATTCAAGGGTATTTTTTGAGCAAGGTGCCACAAGAATTGGTGGCTCTTATAAA
  AAAATGGCATATCGTGAGTACACAGATGGTTCCTTCACAAACCGAAAACAAAGAGGCCCTGATGAGGAAC
  ATCTTGGAATCCTAGGTCCTGTCATTTGGGCAGAAGTAGGAGACACCATTAAAGTCACCTTTCATAACAA
  AGGACAGCATCCTCTCAGCATTCAGCCAATGGGAGTAAGTTTCACTGCAGAAAATGAGGGAACATACTAT
  GGCCCACCAGGTCGCTCCTCACAGCAAGCCTCCCATGTGGCTCCCAAAGAAACCTTTACATACGAATGGA
  CTGTCCCCAAAGAAATGGGACCCACTTATGCAGATCCTGTGTGCCTATCTAAGATGTACTACTCTGGCGT
  TGACCCCACCAAAGATATATTTACTGGGCTTATTGGGCCAATGAAAATATGCAAGAAAGGCAGCTTACTT
  GCTGATGGGAGACAGAAAGATGTAGACAAAGAGTTCTACTTGTTTCCCACAGTGTTTGATGAGAATGAGA
  GTTTACTCTTAGATGATAATATCAGGATGTTCACAACTGCACCTGATCAAGTGGATAAGGAAGATGAAGA
  CTTTCAGGAGTCTAATAAGATGCACTCCATGAATGGGTTCATGTATGGCAATCAGCCTGGCCTCAATATG
  TGTCTAGGAGAATCCATCGTGTGGTATTTGTTCAGCGCTGGAAATGAGGCTGATGTGCATGGGATATACT
  TTTCAGGAAATACTTATCTGTCTAAAGGAGAAAGAAGAGACACTGCAAACCTATTCCCTCATAAAAGTCT
  CACCCTTCTCATGAACCCTGACACAAAAGGGACTTTTGATGTTGAGTGCCTTACAACGGATCACTACACA
  GGTGGCATGAAGCAAAAATACACTGTGAACCAGTGCCAGCGGCAGTTTGAAGATTTCACTGTCTACCTTG
  GAGAAAGGACCTACTATGTGGCAGCCGTAGAGGTGGAATGGGATTACTCACCAAGCAGGGCCTGGGAAAA
  GGAGCTGCATCATTTGCAAGAGCAAAATGTTTCAAATGTATTTTTGGATAAAGAAGAGTTTTTCATAGGC
  TCAAAGTACAAGAAGGTTGTGTATCGCCAGTTTACTGACAGCTCATTCAGAGAACAGGTGAAGAGACGAG
  CCGAAGACGAGCACTTGGGCATCCTTGGGCCACCAATTCATGCAAATGTTGGAGACAAAGTTAAAGTTGT
  CTTTAAAAATATGGCAACCAGGCCATATTCAATACATGCCCATGGGGTGAAAACAGAGAGTTCTACAGTT
  GTTCCAACGTTACCAGGTGAAGTTCGAACTTATACATGGCAAATTCCAGAAAGATCAGGGGCTGGAAGAG
  AGGATTCAGCTTGTATCCCATGGGCTTATTACTCAACTGTGGATCGAGTTAAGGATCTCTATAGTGGGCT
  AATAGGCCCATTGATTGTTTGTCGGAAGTCTTATGTGAAAGTATTCAGTCCTAAAAAGAAAATGGAGTTT
  TTCCTTCTGTTTCTAGTATTTGATGAGAATGAATCTTGGTACTTAGATGATAACATCAAAACATACTCTG
  AACACCCTGAGAAAGTAAACAAAGACAACGAGGAATTCCTAGAAAGCAATAAAATGCATGCTATTAATGG
  GAAAATGTTTGGAAACCTACAAGGCCTCACAATGCACGTGAAAGATGAAGTCAACTGGTATGTGATGGGA
  ATGGGCAATGAAATAGACCTGCACACTGTACACTTCCACGGCCACAGCTTCCAATACAAGCACAGGGGAG
  TATACAGTTCTGATGTCTTTGACCTTTTCCCTGGAACATACCAAACCCTAGAAATGTTTCCCCAAACACC
  TGGAACCTGGTTACTCCACTGCCACGTGACTGACCATGTCCATGCTGGGATGGCAACTACCTACACTGTT
  TTACCAGTAGAACAAGTATCATCTCAGAGTTACAGGATGACCTGGAACATCCTCTATACACTACTAATCA
  GCATCATTATTTTATTCCAAATGTCTACCAAGGAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276248
• Insert Size: 3258 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276248.1, NP_001263177.1
• RefSeq Size: 4561 bp
• RefSeq ORF: 3258 bp
• Locus ID: 12870
• UniProt ID: Q61147
• Cytogenetics: 3 A2
• Gene Summary: The protein encoded by this gene is a copper-containing glycoprotein found soluble in the serum and GPI-anchored in other tissues. It oxidizes Fe(II) to Fe(III) and is proposed to play an important role in iron homeostasis. In humans mutations of this gene cause aceruloplasminemia, which is characterized by retinal degeneration, diabetes, anemia and neurological symptoms. In mouse deficiency of this gene in combination with a deficiency of its homolog hephaestin causes retinal degeneration and serves as a pathophysiological model for aceruloplasminemia and age-related macular degeneration. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (3) uses an alternate, in-frame acceptor splice site as well as an alternate 3' exon structure, compared to variant 2. It encodes isoform c which is longer and has a distinct C-terminus, compared to isoform b. Both variants 3 and 4 encode the same isoform (c).