Pear1 (NM_001032413) Mouse Untagged Clone

CAT#: MC229294

Pear1 (untagged) - Mouse platelet endothelial aggregation receptor 1 (Pear1), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pear1
• Synonyms: 3110045G13Rik; Jedi-1; Megf12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229294 representing NM_001032413
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACTTTGTCCCCTCCTCCTCCTGGCCCTAGGCCTGCGTCTGACTGGGACACTCAACTCCAATGATC
  CCAATGTCTGTACCTTTTGGGAAAGCTTCACCACGACCACTAAGGAGTCCCACCTGCGCCCCTTCAGCCT
  GCTCCCAGCTGAGTCCTGCCACAGGCCCTGGGAGGACCCCCACACCTGTGCCCAGCCTACGGTTGTCTAC
  CGGACTGTGTACCGTCAGGTGGTGAAGATGGACTCCCGCCCACGCCTGCAGTGCTGTAGGGGTTACTACG
  AGAGCAGAGGGGCCTGTGTCCCACTCTGTGCCCAGGAGTGTGTCCATGGTCGCTGTGTGGCTCCGAATCA
  GTGCCAGTGTGCACCAGGCTGGCGGGGTGGCGACTGCTCCAGCGAGTGTGCCCCGGGAATGTGGGGACCA
  CAGTGTGACAAGTTCTGCCACTGTGGCAACAACAGTTCCTGTGATCCCAAGAGTGGGACGTGCTTTTGCC
  CCTCTGGCCTGCAGCCCCCCAACTGCCTTCAGCCCTGCCCTGCCGGCCACTATGGTCCTGCCTGCCAGTT
  TGATTGCCAGTGCTATGGGGCATCCTGTGACCCCCAGGATGGAGCCTGTTTCTGCCCTCCAGGGAGAGCA
  GGACCCAGCTGTAATGTGCCCTGTTCACAGGGCACTGATGGCTTCTTCTGCCCCAGAACCTATCCTTGCC
  AAAATGGAGGTGTTCCTCAGGGCTCCCAAGGCTCCTGCAGCTGCCCACCGGGCTGGATGGGTGTCATTTG
  TTCCCTGCCATGCCCAGAGGGTTTCCATGGACCCAACTGTACTCAGGAATGTCGCTGCCACAACGGTGGC
  CTCTGTGACAGGTTTACTGGGCAGTGCCACTGTGCTCCTGGCTATATCGGGGATCGGTGCCAAGAAGAGT
  GCCCCGTGGGCCGCTTCGGTCAAGACTGTGCTGAGACCTGTGACTGTGCTCCTGGCGCCCGTTGCTTTCC
  TGCTAATGGCGCGTGTCTGTGTGAACATGGCTTCACAGGCGACCGCTGCACTGAGCGCCTCTGTCCGGAT
  GGCCGCTATGGTCTGAGCTGCCAGGAGCCCTGCACCTGCGACCCAGAACACAGTCTCAGCTGCCACCCGA
  TGCACGGCGAGTGCTCCTGCCAGCCAGGTTGGGCGGGCCTCCACTGCAACGAGAGCTGCCCTCAGGACAC
  GCATGGCCCGGGCTGCCAGGAGCACTGCCTCTGTCTGCACGGAGGGCTCTGCCTTGCCGACAGCGGCCTC
  TGCCGGTGCGCGCCGGGATACACGGGACCTCACTGCGCTAACCTATGTCCACCGGACACTTACGGGATCA
  ACTGTTCCTCCCGCTGCTCCTGTGAAAATGCCATTGCCTGCTCTCCCATCGACGGCACGTGCATCTGCAA
  GGAAGGTTGGCAGCGTGGTAACTGCTCTGTTCCCTGTCCCCTTGGCACCTGGGGCTTCAATTGCAATGCC
  AGTTGCCAGTGTGCCCACGACGGAGTCTGCAGCCCCCAAACTGGAGCCTGTACTTGCACCCCTGGGTGGC
  ATGGTGCTCACTGCCAGCTTCCCTGCCCGAAGGGACAGTTTGGTGAAGGCTGTGCCAGTGTCTGTGACTG
  TGACCACTCTGATGGCTGTGACCCTGTTCATGGACAGTGCCGATGTCAGGCTGGTTGGATGGGCACACGC
  TGCCACCTGCCTTGCCCGGAGGGCTTTTGGGGAGCCAACTGCAGTAACACCTGTACCTGCAAGAATGGTG
  GTACCTGTGTGTCTGAGAATGGCAACTGCGTGTGCGCACCAGGGTTCCGAGGCCCCTCCTGCCAGAGGCC
  CTGCCCGCCTGGTCGCTATGGCAAACGCTGTGTGCAATGCAAGTGTAACAACAACCATTCTTCCTGCCAC
  CCATCGGACGGGACCTGCTCCTGCCTGGCGGGCTGGACAGGCCCTGACTGCTCCGAGGCATGTCCCCCAG
  GCCACTGGGGACTCAAATGCTCCCAACTCTGCCAGTGTCATCATGGTGGGACCTGCCACCCCCAGGATGG
  GAGCTGTATCTGCACGCCAGGCTGGACTGGACCCAACTGCTTGGAAGGCTGCCCACCAAGAATGTTTGGT
  GTCAACTGCTCCCAGCTATGTCAGTGTGATCTCGGAGAGATGTGCCACCCAGAGACTGGGGCTTGTGTCT
  GTCCCCCAGGACACAGTGGTGCAGACTGCAAAATGGGAAGCCAGGAGTCCTTCACCATAATGCCCACCTC
  TCCCGTGACCCATAACTCACTGGGTGCAGTGATTGGCATTGCAGTACTGGGAACCCTCGTGGTGGCCCTG
  ATAGCACTGTTCATTGGCTACCGCCAGTGGCAAAAGGGCAAGGAACATGAGCACTTGGCAGTGGCTTACA
  GCACTGGGCGGCTGGATGGCTCTGATTACGTCATGCCAGATGTCTCTCCGAGCTATAGTCACTACTACTC
  CAACCCCAGCTACCACACACTGTCTCAGTGTTCTCCTAACCCCCCACCCCCTAACAAGGTCCCAGGCAGT
  CAGCTCTTTGTCAGCTCCCAGGCCCCTGAGCGGCCAAGCAGAGCCCACGGGCGTGAGAACCATGTCACAC
  TGCCCGCTGACTGGAAGCACCGCCGGGAGCCCCATGAAAGAGGCGCCAGCCACCTGGACCGAAGCTATAG
  CTGTAGCTATAGCCACAGGAATGGCCCGGGACCATTCTGTCATAAAGGTCCCATCTCTGAAGAGGGACTA
  GGGGCAAGCGTTATGTCCCTGAGCAGTGAGAACCCCTATGCTACCATCCGAGACCTGCCCAGCCTGCCTG
  GGGAACCCCGAGAAAGCGGCTATGTGGAGATGAAAGGACCTCCATCAGTGTCCCCTCCCAGGCAGTCTCT
  TCATCTCCGGGACAGGCAGCAGCGGCAACTGCAGCCACAGAGGGACAGTGGCACCTATGAGCAGCCCAGC
  CCCTTGAGCCATAATGAAGAGTCTTTGGGCTCCACGCCCCCGCTTCCTCCAGGCCTGCCTCCTGGTCACT
  ACGACTCCCCCAAGAACAGCCATATCCCTGGACACTATGACTTGCCTCCAGTACGGCATCCTCCATCCCC
  TCCATCCCGGCGCCAGGACCGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001032413
• Insert Size: 3105 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001032413.1, NP_001027585.1
• RefSeq Size: 4367 bp
• RefSeq ORF: 3105 bp
• Locus ID: 73182
• UniProt ID: Q8VIK5
• Cytogenetics: 3 F1
• Gene Summary: When overexpressed, reduces the number of both early and late non-adherent myeloid progenitor cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, and 4 all encode the same isoform (a).