Tbc1d1 (NM_001289514) Mouse Untagged Clone

CAT#: MC229293

Tbc1d1 (untagged) - Mouse TBC1 domain family, member 1 (Tbc1d1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tbc1d1
• Synonyms: 1110062G02Rik; AI385682; AW555803; mKIAA1108; Nob1; Nobq1; Tbc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229293 representing NM_001289514
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACCATTGTTCAGCTGGGTGGCCAAGGTGCCTGAGATCATCAGCTCCATCCGGCAGGCCGGGAAGA
  TTGCCCGCCAGGAAGAGCTGCGTTGCCCCTCCGAGTTCGACGATACCTTCGCCAAAAAGTTCGAGGTGCT
  CTTCTGTGGCCGGGTGACTGTGGCTCACAAGAAGGCCCCACCCGCACTGATTGACGAGTGTATCGAGAAG
  TTCAACCATGTGAGCTGTGGTCGCAGAACGGACTGGGAAGCGCCCACCGGGCAGCCATCAGCGCCTGGCC
  CCAGGCCCATGCGCAAATCCTTCTCACAGCCTGGACTGCGCTCGCTGGCCTTCAGGAAGGAGTTCCAGGA
  CGCTAGCCTCCGCAGTAGCACCTTTAGCTCCTTTGACAATGACATAGAGAACCACCTCATCGGTGGGCAC
  AATGTGGTTCAGCCCACAGACATGGAGGAGAACCGAACTATGCTGTTCACGATTGGCCAATCTGAAGTTT
  ACCTCATCAGTCCTGACACCAAAAAGATTGCACTGGAGAAAAATTTTAAGGAGATATCCTTTTGCTCTCA
  GGGCATCAGACATGTGGACCACTTTGGATTCATCTGCCGAGAGTGCTCGGGTGGCGGCAGTGGCGGCTTT
  CATTTTGTCTGTTACGTGTTCCAGTGCACAAATGAAGCTCTGGTTGACGAGATCATGATGACTCTGAAGC
  AGGCTTTCACGGTAGCTGCGGTGCAGCAGACGGCTAAGGCACCAGCCCAGCTCTGTGAGGGCTGCCCCTT
  GCAAGGCCTGCACAAGCTCTGCGAAAGGATAGAGGGAATGAATTCATCTAAAACCAAATTAGAACTCCAG
  AAGCACTTGACCACACTGACCAATCAGGAGCAGGCCACCATATTCGAGGAGGTTCAGAAATTGAGACCAA
  GAAACGAGCAGCGAGAGAATGAATTAATTATTTCTTTTCTGAGGTGCTTATATGAAGAGAAGCAAAAAGA
  GCACAGCCACACTGGGGAGCCAAAGCAGACACTACAGGTGGCAGCAGAGAATATTGGGAGTGACCTGCCA
  CCCAGTGCTAGCCGGTTCAGGTTAGATTCGCTGAAGAACAGAGCAAAGAGGTCCTTAACAGAGTCCCTAG
  AGAGCATTCTGTCCCGGGGTAATAAAGCCAGAGGCCTGCAGGACCATTCCGCCAGTGTGGATCTGGACAG
  CTCCACTTCTAGTACTCTAAGTAACACCAGCAAAGAGCTGTCCATGGGTGACAAGGAGGCCTTCCCCGTC
  TCTGAGACCTCCTTCAAGCTCCTTGGCTCCTCAGATGACCTGTCCAGTGACTCAGAGGGCCACATTGCAG
  AAGAGTCTGCCCTGTTGTCACCCCAGCAGGCGTTCAGAAGGAGAGCCAACACCCTGAGTCATTTCCCAGT
  AGAGTGCCCTGCGCCTCCAGAACCTGCCCAGAGCTCTCCAGGGGTCTCTCAAAGGAAACTCATGCGGTAC
  CACTCCGTGAGCACAGAGACGCCTCATGAACGCAAGGACTTTGAATCCAAAGCAAACCACCTGGGTGACA
  CAGATGGGACCCCCGTGAAGACCCGGCGGCACTCGTGGAGACAGCAGATATTCCTTCGAGTGGCCACTCC
  ACAGAAGGCTTGTGACTCCCCGAGCAGATATGAAGATTATTCCGAGCTGGGAGAGCTCCCTCCACGCTCC
  CCTTTAGAACCGGTGTGTGAGGACGGCCCATTTGGCCCAGTACAGGAAGAAAAGAGGAAGACGTCACGCG
  AGCTTCGAGAGCTGTGGAAAAAGGCCATCTTGCAGCAGATCCTGCTGCTCAGGATGGAGAAGGAGAATCA
  GAAGCTACAAGCCTCTGAAAACGATTTGCTGAACAAACGCCTCAAGCTTGACTATGAAGAAATCACTCCG
  TGTCTTAAAGAAGTCACTACAGTGTGGGAAAAGATGCTTAGCACTCCAGGAAGATCCAAAATTAAGTTTG
  ACATGGAAAAAGTGCACTCAGCTGTTGGGCAAGGTGTGCCACGTCATCACCGAGGTGAGATCTGGAAATT
  TCTAGCTGAGCAGTTCCACCTTAAACACCCATTTCCTAGTAAACAGCAGCCAAAGGACGTGCCCTACAAA
  GAGCTCCTGAAGAAGCTGACCTCGCAGCAGCACGCCATTCTCATCGACCTCGGGCGAACCTTTCCAACAC
  ATCCATACTTCTCTGCCCAGCTTGGAGCAGGTCAGCTGTCACTTTACAACATTCTGAAGGCCTACTCGCT
  TCTGGACCAGGAGGTTGGATACTGCCAAGGTCTCAGCTTTGTGGCAGGCATTTTGCTTCTTCACATGAGT
  GAGGAAGAGGCGTTCAAGATGCTCAAGTTCCTGATGTTTGACATGGGGCTGCGGAAACAGTATCGGCCAG
  ACATGATTATTTTGCAGATCCAGATGTACCAGCTGTCACGGCTCCTCCACGATTACCACCGAGACCTCTA
  CAACCACCTGGAAGAGCACGAGATCGGCCCCAGCCTCTACGCGGCTCCCTGGTTTCTCACTGTGTTCGCC
  TCACAGTTCCCACTCGGCTTTGTAGCCAGAGTCTTTGATATGATCTTCCTTCAGGGATCAGAGGTCATAT
  TTAAAGTAGCTTTAAGTCTTTTGGGGAGCCATAAGCCCTTGATTCTACAGCATGAGAACCTGGAAACCAT
  AGTGGACTTCATAAAGAACACACTCCCCAACCTGGGCCTGGTGCAGATGGAGAAGACCATCAGTCAGGTG
  TTTGAGATGGACATCGCCAAGCAGCTCCAGGCCTATGAGGTCGAGTACCACGTGCTCCAGGAGGAGCTTA
  TTGAGTCCTCGCCTCTCAGTGACAACCAAAGAATGGAGAAATTGGAGAAAACCAACAGCAGCTTGCGCAA
  ACAGAACCTTGACCTCCTGGAGCAGTTGCAGGTGGCAAATGCTAGGATCCAAAGCCTTGAAGCCACGGTA
  GAGAAACTTCTTACCAGCGAGAGTAAGCTGAAGCAGGCTGCGCTGACCCTGGAGGTGGAGCGCTCCGCCC
  TGCTGCAGATGGTGGAGGAGCTGCGGAGGCAAAGCGCCCGGCCCAGCACTCCAGAGCCAGACTGCACCCA
  GCTGGAGCCCACAGGCGATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289514
• Insert Size: 3102 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289514.1, NP_001276443.1
• RefSeq Size: 4802 bp
• RefSeq ORF: 3102 bp
• Locus ID: 57915
• Cytogenetics: 5 32.8 cM
• Gene Summary: May act as a GTPase-activating protein for Rab family protein(s). May play a role in the cell cycle and differentiation of various tissues. Involved in the trafficking and translocation of GLUT4-containing vesicles and insulin-stimulated glucose uptake into cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.