Lig3 (NM_001291245) Mouse Untagged Clone

CAT#: MC229272

Lig3 (untagged) - Mouse ligase III, DNA, ATP-dependent (Lig3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lig3
• Synonyms: D11Wsu78e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229272 representing NM_001291245
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTTGGCTTTCAAGATCCTCTTCCCGAGAAACCTTTGTGCCCTTGGCAGAAAAGAACTGTGCCTGT
  TCCCAGAACAGAATCGCTGGGCTGCCATAAGTCAGTTCAGCCAGTGGTCGGAAACAAATCTCCTCGGTGG
  GTGCTGCCTTCTCCAGAGAAGAAAGCCTGTCCTAGCTCTCCAGAGAGGTCATCTAAGACCACGTGCCACC
  CACCTTACTTTCTGGCCAGGGTCGCATGTGGGACTCTGTACTGGCCCCTGTGCGATGGCAGAGCAGCGGT
  TCTGTGTGGACTATGCCAAGCGGGGCACAGCTGGATGCAAGAAATGCAAGGAGAAGATTGTAAAGGGCGT
  GTGCCGCATTGGCAAAGTGGTGCCCAACCCCTTCTCAGAGTCTGGGGGTGATATGAAAGAGTGGTACCAC
  ATTAAGTGCATGTTTGAGAAACTGGAACGGGCTCGGGCCACCACAAAGAAAATTGAAGACCTCACAGAGC
  TGGAAGGCTGGGAAGAGCTGGAAGATAACGAAAAGGAACAGATAAGCCAGCATATTGCAGACCTGTCTTC
  TAAGGCAGCTGCCACACCTAAGAAGAAAGCTGCTGTCCAGGCTAAGCTGACAACCACTGGCCAGGTGACA
  TCTCCAGTGAAAGGTGCTTCGTTTATTACCAGTACCAATCCTCGGAAGTTCTCTGGCTTTTCAGCAGCAA
  AACCCAACAACTCTGAGCAAGCCCCCTCAAGCCCTGCTCCTGGAACGAGTCTGTCTGCAAGTAAATGTGA
  CCCTAAGCACAAAGATTGTCTGCTACGAGAGTTCCGGAAGCTGTGCGCCATGGTGGCTGAAAATCCTAGC
  TATAACACCAAGACCCAGATCATCCATGACTTCTTGCAGAAAGGCTCCACAGGAGATGGCTTTCACGGTG
  ATGTGTACCTGACAGTTAAGCTGCTGCTGCCTGGTGTCATCAAGAGTGTTTACAACTTGAATGATAAGCA
  GATTGTAAAACTTTTTAGCCGAATTTTTAACTGCAACCCAGATGATATGGCCCGGGACCTAGAACAGGGT
  GACGTGTCAGAGACGATCAGAATCTTCTTTGAGCAGAGCAAGTCTTTCCCCCCAGCTGCCAAAAGCCTCC
  TCACCATTCAGGAAGTGGATGCCTTCCTCCTGCACCTCTCCAAGCTCACCAAAGAGGATGAGCAGCAGCA
  GGCCCTGCAGGACATAGCCTCCCGGTGTACAGCCAATGACCTTAAGTGCATTATTCGGCTGATCAAACAT
  GATCTGAAGATGAACTCGGGTGCAAAACACGTGTTAGATGCCCTTGACCCCAATGCTTATGAAGCCTTCA
  AAGCATCGCGCAACCTGCAGGATGTGGTGGAGCGAGTCCTTCACAATGAGCAGGAGGTGGAGAAGGACCC
  AGGCCGGCGACGGGCTCTGAGGGTCCAGGCCTCACTGATGACGCCTGTGCAGCCCATGCTGGCTGAGGCC
  TGCAAATCCATTGAATATGCAATGAAGAAGTGTCCCAATGGCATGTTCTCTGAGATCAAGTACGATGGTG
  AGCGAGTCCAGGTGCATAAGAAGGGGGACCACTTCAGCTACTTCAGCCGCAGTCTCAAGCCTGTCCTGCC
  TCACAAGGTGGCCCACTTCAAGGACTATATACCCAAAGCTTTTCCTGGGGGTCAAAGCATGATCTTGGAT
  TCCGAGGTACTCCTGATTGACAACAACACAGGCAAACCACTGCCCTTTGGGACTCTGGGAGTGCACAAGA
  AAGCTGCTTTCCAGGATGCTAATGTCTGCCTGTTTGTTTTTGATTGTATCTACTTTAATGATGTCAGCTT
  GATGGACAGGCCTCTCTGTGAGCGACGGAAGTTTCTTCATGACAACATGGTTGAAATCCGTAACCGGATC
  ATGTTCTCAGAAATGAAGCAAGTCACAAAAGCTTCAGACCTGGCTGACATGATAAACCGGGTGATCCGAG
  AGGGCTTGGAAGGGTTGGTGCTAAAGGATGTGAAGGGTACATATGAGCCTGGGAAGCGGCACTGGCTGAA
  AGTTAAGAAAGATTATTTGAATGAGGGGGCCATGGCTGATACAGCTGATCTGGTGGTTCTTGGGGCCTTC
  TATGGGCAAGGGAGCAAAGGCGGAATGATGTCCATCTTCCTCATGGGCTGCTATGACCCTGACAGCCAAA
  AGTGGTGCACTGTTACAAAATGTGCTGGAGGCCATGATGATGCCACACTTGCCCGCCTGCAGAAAGAGCT
  GGTTATGGTGAAGATCAGCAAGGATCCTAGTAAGATACCCAGCTGGCTGAAGATCAACAAGATCTATTAT
  CCTGACTTTATCGTCCCAGACCCAAAGAAAGCTGCTGTGTGGGAGATCACAGGGGCAGAATTCTCCAGAT
  CTGAGGCACACACTGCTGATGGCATCTCCATCCGATTTCCTCGATGCACCCGAATCCGGGACGACAAGGA
  CTGGAAATCTGCCACTAACCTCCCCCAACTCAAGGAACTGTACCAGCTGTCCAAAGACAAGGCAGACTTT
  GCTGTGGTGGCTGGAGATGAGGCAAGCCCCACTACTGGAGGCAGCAGTGGCGAGAACGAGGGCACTGCTG
  GGTCTGCTGGGCCCTGCAAGGGCCCCCCCAGTAAGTCCTCTGCCAGTGCCAAGACCACCGAACAGAAGCT
  CAATAGCCCCAGTAGCAGAGGTGGCATCAAACCGATTCCAAAGCATTCCCCCATGAAACCAGGAGAGAAG
  CTGGCTGTGAAGTCTTCTCCAGTCAAAGTGGGAATGAAGAGGAAAGCTACTGATGAGACCCCATGCCTGA
  AAAAGGTACTGTTGGATGTCTTCACTGGGGTGCGGCTCTACTTGCCACCTTCTACACCAGACTTCAAACG
  TCTCAAACGCTACTTTGTGGCATTCGACGGGGACCTGGTACAGGAATTTGACATGGGCTCAGCCACACAT
  GTGCTAGGTAACAGGGAAAAGAACACTGATGCCCAGTTGGTCTCCTCAGAGTGGATTTGGGCATGTATCC
  GGAAACGGAGGCTGATAGCTCCCTGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291245
• Insert Size: 3039 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291245.1, NP_001278174.1
• RefSeq Size: 5984 bp
• RefSeq ORF: 3039 bp
• Locus ID: 16882
• Cytogenetics: 11 50.23 cM