Arvcf (NM_001272030) Mouse Untagged Clone

CAT#: MC229219

Arvcf (untagged) - Mouse armadillo repeat gene deleted in velo-cardio-facial syndrome (Arvcf), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Arvcf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229219 representing NM_001272030
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGACTGCAACGTGCATTCGGCCGCCAGCATTTTGGCCTCGGTGAAGGAACAGGAGGCCCGTTTCG
  AGCGGCTGACACGGGCACTAGAACAAGAACGGCGCCATGTTGCCCTGCAGCTGGAGCGTGCCCAGCAGCC
  TGGCATGAGCAGTGGTGGTATGGTGGGCAGTGGGCAGCCCCTGCCAATGGCCTGGCAGCAGCTGGTTCTG
  CAGGAGCAGAGCCCGGGTAGCCAGGCATCGCTGGCCACGATGCCAGAGGCACCCGAGGTACTGGAGGAGA
  CAGTGACTGTGGAGGAAGACCCTGGCACCCCCACCTCTCACGTGTCCATCGTCACATCAGAAGATGGTAC
  AACCCGGCGGACTGAGACTAAGGTCACTAAGACGGTCAAGACTGTGACCACAAGGACAGTACGCCAGGTG
  CCTTTGGGCCCAGATGGACTCCCCTTGCTGGACGGCGGCCCTCCACTTGGCTCTTTTGCTGACGGGCCCC
  TGGACCGGCATTACCTACTTCGTGGTGGTGGTGGTCCAGCAGCCACACTCTCCCGAACCTACCACAGCAG
  TGGAGGTGGCTTTCCCGATGGCCCCGAGTCCCGTGATATTCCAAGCTATGGCAGCTTGTCCCGAGGGCTC
  GGGGTACGGCCACCACGTACTGGCCTCCTGGGCCCAGGACCTGGTGATGGCTGTTTCACACTGCCTGGCC
  GCCGAGAAGCCTTCCCCATGGGCTCTGAATCCGGACCACCAAGCGGCCGCTCTCTGCCTGAGCATTTCCA
  AGCTGAACCATATGGCCTGGAGGATGATACACGGAGCCTGGCTGCGGATGATGAGGGTGGCCCCGACCTG
  GAGCCTGACTATAGCACGGCAACCCGGAGAAGACCTGAGTACGGGCGGGGCCTTCGTGCCAGGGCCTTTG
  AAGACACAGCAGATGATGCTGGTGAGCTGATAGAAGAGCGTCCCCCATTTCCAGCAGCAACAGCCCCTCT
  GGCCCAGCCTGAGAGGGGCAGCCTGGGCAGCTTGGACCGAGTAGTGCGGCGCTCACCTTCAGTGGATAGC
  ACCCGCAAGGAGCCACGCTGGCGGGACCCCGAGCTGCCAGAGGTGTTGGCCATGCTGCGGCACCCTGTGG
  ACCCTGTGAAAGCCAATGCTGCAGCCTACCTGCAGCACCTCTGCTTTGAGAACGAGGGTATTAAGCGGCG
  AGTGCGGCAGCTGCGTGGGCTCCCCCTGCTTGTGGCATTACTAGATCACCCTCGGGCTGAGGTGCGGCGC
  CGGGCCTGTGGGGCACTGCGCAACCTCTCCTATGGCCGGGATACTGACAACAAGGCCGCCATCCGGGACT
  GCGGGGGCGTGCCAGCCCTGGTGCGCCTACTGCGGGCGGCCCGTGACAATGAGGTCCGTGAGCTGGTCAC
  TGGCACACTCTGGAACCTGTCATCCTACGAGCCCCTGAAGATGGTCATCATTGACCACGGCTTACAGACT
  CTGACCCATGAGGTCATCGTGCCCCATTCCGGCTGGGAGCGAGAGCCTAACGAAGACTCGAAGCCCCGGG
  ATGCCGAGTGGACAACAGTCTTCAAGAACACATCAGGCTGCCTGAGGAATGTGAGCTCAGATGGTGCAGA
  GGCCCGGCGGCGACTCCGTGAGTGCGAAGGGCTGGTGGACGCTCTCCTACATGCCCTGCAGTCAGCTGTG
  GGCAGGAAAGACACAGATAATAAGTCAGTAGAGAACTGTGTGTGCATCATGCGGAACCTGTCCTACCACG
  TGCACAAAGAAGTTCCAGGGGCTGACAGGTACCAGGAGGCAGAGCCTGGGATCCCGGGCAGCACAACCTC
  CCAGCGACGGAGGAAGGATGACGCCAGCTGCTTTGGTGGCAAGAAAGCAAAAGGGAAGAAGGATGCAGAG
  ATGGACCGGAACTTTGACACACTGGACCTGCCTAAACGAACGGAGGCTGCAAAAGGCTTCGAGCTGCTGT
  ACCAGCCGGAGGTGGTACGTCTCTACCTCTCACTCCTTACGGAGAGCCGGAACTTCAACACCCTGGAAGC
  TGCAGCCGGTGCCCTGCAAAACCTCAGTGCTGGCAACTGGACGTGGGCCACGTACATCCGTGCCACAGTG
  CGCAAGGAACGTGGGCTGCCAGTACTGGTGGAACTGCTACAGTCTGAGACCGACAAGGTGGTGCGCGCTG
  TAGCCATCGCGCTGCGCAACCTCTCACTAGACCAGCGGAACAAAGACCTCATCGGGAGCTATGCCATGAC
  AGAGCTGGTTCGGAATGTTCGCAATGCACAGGCTCCCGCTCACCCCAGTGCCCACCTGGAGGAGGATACT
  GTGGTTGCCGTGCTCAACACCATCCATGAGATCGTGTCCGACAGCCTGGACAATGCTCGCTCCCTCCTGC
  AGGCCCGTGGTGTGCCTGCCCTGGTAGCACTTGTGGCCTCCAGCCAGTCAGTGCGGGAGGCCAAGGCAGC
  ATCACACGTGCTACAGACTGTATGGAGCTACAAGGAGCTGCGTGGAGCCCTGCAGAGGGATGGCTGGACG
  AAGTCACGCTTCCAGTCTGCTAGCACTGCCAAAGGACCCAAAGGGACACCAAGTTCTGGCGGCTTTGATG
  ACAGCACACTGCCACTGGTAGACAAGAGCCTTGATGGGGAGAAGTCAAACACCCGGGATGTGATCCCCAT
  GGATACCCTTGGTCCAGATGGTTACGCCACAGTTGACCGGAGGGAGAGGAGGACACTGGGCAGTGACTCC
  ACAGGGGACACCTCTGAGAAAGAACTATTGAGACCCGATCCTGGCAGGAAGGCCCCTCCGCCTGGGCCCA
  GCAGGCCCTCGGTCAGGCTGGTGGACGCCGTGGGGGACACTAAGCCTCAGCCTGTTGACTCCTGGGTCTA
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001272030
• Insert Size: 2871 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001272030.1, NP_001258959.1
• RefSeq Size: 4569 bp
• RefSeq ORF: 2871 bp
• Locus ID: 11877
• UniProt ID: P98203
• Cytogenetics: 16 11.37 cM
• Gene Summary: Involved in protein-protein interactions at adherens junctions.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) differs in the 5' UTR and in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Both variants 3 and 4 encode the same isoform.