Slc8a1 (NM_001286684) Mouse Untagged Clone

CAT#: MC229191

Slc8a1 (untagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 1 (Slc8a1), transcript variant C

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc8a1
• Synonyms: AI852629; AV344025; D930008O12Rik; Ncx1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229191 representing NM_001286684
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCGATTAAGTCTCCCACCCAATGTTTCAATGGGATTTCGTCTGGTAGCTCTGGTGGCTCTCTTGT
  TTTCCCATGTTGACCATATAACTGCAGATACAGAGGCAGAAACAGGAGGAAATGAAACCACTGAATGTAC
  TGGCTCATATTACTGTAAGAAAGGGGTGATCTTGCCCATTTGGGAACCCCAAGACCCATCTTTTGGGGAC
  AAAATTGCTAGAGCAACTGTGTATTTTGTGGCCATGGTCTACATGTTCCTTGGAGTTTCTATTATTGCAG
  ACCGGTTTATGTCCTCTATAGAGGTCATCACCTCTCAAGAGAAAGAAATAACGATAAAGAAACCGAATGG
  AGAGACCACCAAGACGACGGTGAGAATCTGGAACGAGACTGTGTCGAACCTGACCTTGATGGCCCTGGGA
  TCTTCTGCTCCTGAGATTCTCCTGTCAGTCATTGAAGTGTGCGGCCATAACTTCACCGCAGGGGACCTGG
  GTCCCAGCACCATCGTGGGAAGTGCTGCCTTTAACATGTTCATCATAATCGCACTCTGTGTTTACGTGGT
  CCCTGATGGAGAGACAAGGAAGATCAAGCATCTGCGTGTGTTCTTTGTGACAGCAGCCTGGAGCATCTTT
  GCCTATACCTGGCTTTATATAATCTTGTCTGTCAGCTCTCCTGGAGTTGTGGAGGTCTGGGAAGGCTTGC
  TTACTTTCTTCTTCTTTCCCATCTGCGTTGTGTTCGCGTGGGTAGCAGACAGGCGGCTTCTCTTTTACAA
  GTATGTCTACAAGCGGTACAGGGCCGGCAAGCAGAGGGGGATGATCATTGAACATGAAGGAGACAGACCA
  GCTTCCAAAACTGAAATCGAAATGGATGGGAAAGTGGTCAACTCTCATGTTGACAATTTCTTAGATGGGG
  CTCTGGTTTTGGAAGTTGATGAGAGGGACCAAGATGATGAGGAAGCCAGGCGTGAGATGGCAAGGATTCT
  GAAGGAACTTAAGCAGAAGCATCCTGAGAAAGAAATTGAGCAATTAATAGAATTAGCCAACTACCAGGTC
  CTAAGTCAACAGCAGAAAAGCCGAGCATTTTACAGGATTCAAGCTACTCGCCTGATGACCGGAGCTGGCA
  ACATCTTGAAGAGGCACGCAGCTGATCAAGCAAGGAAGGCTGTCAGTATGCATGAAGTCAACATGGAAAT
  GGCTGAAAACGACCCAGTCAGTAAGATCTTCTTTGAGCAAGGAACATACCAGTGTCTAGAGAACTGTGGT
  ACTGTGGCCCTCACCATTATGCGCAGAGGGGGCGACTTGAGCACCACTGTGTTTGTTGACTTCAGGACAG
  AAGACGGCACAGCCAATGCTGGGTCTGATTATGAATTCACGGAAGGGACTGTGATCTTCAAACCAGGGGA
  GACCCAGAAGGAAATCAGAGTTGGCATCATTGATGATGATATCTTTGAAGAAGATGAAAACTTCCTTGTG
  CATCTTAGCAATGTCAGAGTCTCTTCAGATGTTTCAGAAGATGGCATACTAGAATCCAATCACGCTTCTT
  CAATTGCTTGTCTTGGGTCACCCAGCACTGCCACCATAACCATTTTTGATGATGACCATGCAGGCATCTT
  TACATTTGAGGAACCCGTGACTCACGTGAGCGAGAGCATTGGCATCATGGAGGTGAAGGTTTTGAGAACC
  TCTGGAGCTCGAGGAAATGTTATCATTCCCTACAAAACTATTGAAGGCACAGCCCGAGGTGGAGGGGAAG
  ACTTTGAGGACACCTGTGGAGAGCTCGAATTCCAGAACGATGAAATAGTCAAAACAATATCAGTCAAGGT
  AATCGATGACGAGGAGTATGAGAAAAACAAGACCTTCTTCATTGAGATTGGAGAGCCCCGTCTGGTGGAG
  ATGAGTGAGAAGAAAGCCCTGTTGTTGAATGAGCTTGGTGGCTTCACATTAACAGGAAAAGAGATGTATG
  GCCAACCTATCTTCAGGAAGGTCCATGCTAGAGATCATCCGATTCCCTCTACTGTAATCACCATCTCAGA
  GGAATATGATGACAAGCAGCCACTGACCAGCAAAGAAGAGGAGGAGAGGCGCATTGCGGAAATGGGGCGC
  CCCATCCTAGGCGAGCACACCAAGCTGGAGGTGATCATCGAAGAGTCTTACGAATTCAAGAGCACTGTGG
  ACAAACTCATTAAGAAGACGAACCTGGCCCTTGTGGTGGGGACCAACAGCTGGAGAGAGCAGTTTATCGA
  AGCCATCACTGTCAGCGCTGGGGAAGATGACGATGATGATGAATGTGGGGAGGAGAAGCTGCCCTCCTGT
  TTTGATTACGTGATGCACTTTCTCACAGTGTTCTGGAAGGTTCTGTTTGCCTTCGTCCCACCTACAGAAT
  ACTGGAATGGCTGGGCCTGCTTCATTGTCTCCATCCTCATGATCGGCCTACTGACCGCCTTCATTGGAGA
  CCTGGCTTCCCACTTTGGCTGCACCATTGGTCTGAAAGATTCCGTGACTGCCGTTGTGTTTGTTGCTCTT
  GGAACCTCGGTGCCAGACACATTTGCCAGCAAAGTAGCAGCTACCCAGGACCAGTATGCAGATGCGTCTA
  TAGGCAATGTCACTGGAAGCAATGCTGTGAATGTCTTCCTGGGAATCGGCGTGGCCTGGTCCATTGCTGC
  CATCTACCATGCGGCCAACGGGGAACAGTTCAAAGTGTCCCCCGGCACGCTAGCTTTCTCTGTCACTCTC
  TTCACTATTTTTGCTTTCATCAACGTGGGGGTGCTGCTGTATCGGCGGAGGCCAGAAATAGGAGCCATCT
  TCGGAATGTCAATAATTCTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286684
• Insert Size: 2823 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286684.1, NP_001273613.1
• RefSeq Size: 15847 bp
• RefSeq ORF: 2823 bp
• Locus ID: 20541
• Cytogenetics: 17 51.29 cM
• Gene Summary: Mediates the exchange of one Ca(2+) ion against three to four Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes (PubMed:8659820). Contributes to Ca(2+) transport during excitation-contraction coupling in muscle. In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A1 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline (PubMed:10967099). Required for normal embryonic heart development and the onset of heart contractions (PubMed:10967099).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (C) lacks an internal segment in the 3' terminal exon, which results in an alternate stop codon, compared to variant A. The resulting isoform (C) has a shorter and distinct C-terminus, compared to isoform A. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.