Osbpl6 (NM_001290734) Mouse Untagged Clone

CAT#: MC229186

Osbpl6 (untagged) - Mouse oxysterol binding protein-like 6 (Osbpl6), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Osbpl6
• Synonyms: 1110062M20Rik; AI596402; mKIAA4128; ORP-6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229186 representing NM_001290734
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCAGACGAGAAGGGCATATCCCCTGCTCATAAAACATCCACTCCGACCCATAGAAGTGCCTCGT
  CTTCAACATCTTCTCAGAGGGAAAGCAGACAGAGCATCCACGTTTTGGAGAGAACTGCTTCATCCAGCAC
  GGAGCCCTCTGTCAGTCGGCAGTTGCTAGAGCCGGAGCCCATCCCCCTCTCCAAGGAAGCTGACAGCTGG
  GAAATTATAGAAGGGCTGAAAATAGGCCAAACCAATGTCCAGAAACCAGACAGGCACGAGGGATTTATGC
  TGAAGAAAAGAAAATGGCCTTTAAAGGGCTGGCACAAGCGCTTTTTTGTCCTGGATAATGGAATGCTGAA
  GTATTCCAAGGCGCCACTTGATATCCAAAAAGGAAAAGTGCACGGGAGCATAGATGTCGGACTGTCTGTC
  ATGTCGATTAAAAAGAAAGCTCGGAGGATAGACCTTGACACGGAAGAGCACATCTATCATTTGAAGGTGA
  AATCCCAGGACTGGTTTGACGCTTGGGTCTCCAAACTGCGCCATCACAGGTTGTACCGTCAGAATGAAAT
  TGTGAGGTCGCCGAGAGATGCCAGTTTCCACATATTTCCTGCAACTTCGACAGCTGAGTCCTCCCCGGCT
  GCTAATGTCTCTGTTGTGGATGGAAAGATGCAGCCCAACAGCTTTCCATGGCAGTCGCCATTACCATGCA
  GTAACAGCCTCCCTGCGACCTGCACAACTGGGCAGAGTAAAGTGGCAGCCTGGCTGCAGGACTCAGAAGA
  AATGGACAGGTGTGCAGAAGATCTTGCACACTGCCAGTCAAACCTTGTGGAACTTAGCAAGCTCCTGCAA
  AACTTGGAGATCCTTCAGAGAACTCAGTCGGCGCCTAACTTCACTGACATGCAGGAAGGGCCACCTGCGA
  AGGGCCAGTTCAACACAACTCGGCGCCGGCAGAGGCTAGCGGCAGCAGTGGCTACAACAGTTCCTTTCAG
  CGCCACCATGTCACCCGTGCGTCTGCACTCCTCCAACCCCAACCTTTGTGCAGACATTGAGTTCCAGACC
  CCTCCTAGCCACCTCACCGACCCTCTGGAAAGTTCCACGGATTATACCAAGCTACAAGAAGAATTTTGTC
  TAATTGCACAGAAAGTGCATTCTCTTCTGAAGTCTGCATTTAACAGCATAGCTATAGAGAAGGAGAAGCT
  GAAGCAGGTGGTCTCGGAGCAAGATCACAACAAAGGCCACAGCACACAGATGGCCAGACTCCGGCAGTCA
  CTGTCACAGGCACTTAACCAGAACGCTGAGCTCCGGAGTCGGCTGAACAGAATACACTCAGAGTCTACCA
  TTTGTGATCACGTTGTCAGTGTAAATATTATCCCTAGCCCCGATGAGCCTGGTGAGCAAATCCATGTCAG
  CCTCCCTCTGTCACAGCAGGTGGCCAACGAGAGCCGCCTCTCCATGTCAGAATCCGTGTCAGAATTCTTC
  GATGCCCAAGAGGTGCTCCTGTCTGCAAGCTCATCAGAGAATGAGGCTTCTGATGATGAGTCCTACATCA
  GCGACGTGAGTGACAACATATCTGAGGACAACACCAGTGTCGCCGACAACATTTCTCGGCAAATCCTGAA
  TGGGGAGCTCACAGGAGGCGCCTTCCGCAATGGCCGTCGCACCTGCCTGCCTGCTCCTTGCCCGGACACC
  AGTAACATTAACCTGTGGAATATCTTGAGGAACAACATTGGCAAAGACCTGTCGAAGGTCTCCATGCCCG
  TGGAACTCAACGAGCCCCTCAACACCCTGCAGCACCTCTGTGAGGAAATGGAATACAGTGAGCTCCTGGA
  CAAGGCCTCAGAGACCGACGACCCCTACGAGAGGATGGTTCTCGTCGCTGCCTTTGCAGTCTCAGGGTAC
  TGTTCCACCTACTTCCGAGCAGGAAGCAAGCCATTCAATCCGGTCCTGGGGGAGACCTATGAATGCATAA
  GAGAAGATAAGGGGTTTCGGTTCTTCTCAGAACAAGTTAGCCATCATCCACCCATTTCTGCCTGTCACTG
  TGAATCCAAGAATTTTGTGTTTTGGCAAGATATCAGATGGAAAAATAAGTTCTGGGGAAAGTCCATGGAA
  ATCCTGCCTGTCGGAACCCTGAACGTCACGCTTCCAAAATATGGGGATTACTATGTGTGGAATAAAGTCA
  CCACGTGCATACACAATATCCTGAGTGGGAGGAGATGGATCGAGCACTACGGTGAAGTGACCCTTAGAAA
  TACCAAAAGCAGTGTTTGCATTTGCAAACTCACGTTTGTCAAGGTGAATTACTGGAACTCCAACGTGAAT
  GAAGTCCAGGGGGTGGTGATAGACCAGGAGGGGAAGGTGGTGCACCGGCTGTTTGGGAAATGGCACGAAG
  GACTCTACTGCGGGGTGGCCCCCTCGGCAAAGTGCATCTGGAGACCAGGTTCTCTGCCAACCAACTATGA
  GCTCTACTATGGCTTTACAAGATTTGCTGTGGAGCTCAATGAGTTGGACCCCGTCCTGAAGGACCTCCTT
  CCCCCCACAGATGCCCGATTCCGGCCAGATCAAAGGTTTTTGGAAGAAGGGAACCTAGAAGCCGCAGCCG
  CAGAGAAACAAAGAGTTGAGGAACTCCAAAGATCGCGAAGACGCTACATGGAGGAAAACAACCTTGAACA
  TATACCAAAATTTTTTAAAAAAGTTATTGATGCAAATCAAAGAGAAGCCTGGGTTTCTAACGACACCTAC
  TGGGAGCTTCGAAAGGACCCTGGGTTTAGCAAAGTAGACAGCCCCGTTCTTTGGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290734
• Insert Size: 2787 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290734.1, NP_001277663.1
• RefSeq Size: 7968 bp
• RefSeq ORF: 2787 bp
• Locus ID: 99031
• UniProt ID: Q8BXR9
• Cytogenetics: 2 C3
• Gene Summary: Weakly binds 25-hydroxycholesterol.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in both the 5' and central coding regions, compared to variant 1, resulting in an isoform (c) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.