Usp33 (NM_001252486) Mouse Untagged Clone

CAT#: MC229142

Usp33 (untagged) - Mouse ubiquitin specific peptidase 33 (Usp33), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp33
• Synonyms: 9830169D19Rik; AA409780; Vdu1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229142 representing NM_001252486
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGACTTTTCGAAATCATTGTCCACATTTGGATTCAGTTGGTGAAATAACGAAAGAGGACTTGATAC
  AGAAATCTCTCGGCGCTTGTCAGGACTGTAAAGTCAGAGGACCAAACCTGTGGGCGTGCCTGGAGAATAG
  GTGCTCCTATGTTGGCTGCGGGGAGTCGCAAGTGGACCACAGCACCATACACTCGCAGGAGACAAAGCAT
  TATCTAACTGTGAACCTCACCACTCTTCGAGTATGGTGTTATGCTTGCAGCAAAGAAGTATTTTTGGATA
  GAAAATTAGGAACTCCTCCTTCATTACCTCATGTAAGACAGCCTCAACAAACACAAGAAAACAGTGTCCA
  GGATTTTAAAATTCCCAGTAATCCAGCATTGAAAACCCCCATGGTTGCTGTGTCTGAAGATCTGGATATA
  GAAGTGGAAGAGGAAGACGAGCTGAAGGCTAGAGGCCTGACAGGTTTGAAAAACATTGGAAATACTTGTT
  ATATGAACGCGGCGCTGCAGGCTCTTTCTAACTGCCCACCTTTGACACAGTTCTTTCTTGATTGTGGAGG
  ACTGGCTAGAACAGATAAGAAACCAGCAATTTGTAAAAGTTATCTCAAACTAATGACCGAGTTGTGGCAC
  AAGAGCAGGCCAGGATCTGTTGTGCCGGCTAATCTGTTCCAAGGAATTAAAACTGTAAATCCAACTTTTC
  GAGGTTATTCTCAGCAGGATGCCCAGGAATTCCTTCGCTGTCTAATGGACCTGCTTCATGAGGAGCTGAA
  GGAGCAGGTCATGGAAATGGAGGAAGAGCCTCAAACACTAACTTCTGAGGAGACGGTGGAGGAAGAGAAG
  AGCCAGTCAGATGTGGATTTTCAGTCGTGCGAGTCTTGTAGCAGCAGCGAGAAAGCAGAAAATGAGAGTG
  GCTCCAAAGGCTTTCCTGAGGACAGCAATGAGACCACCATGCTCATCCAGGACGAGGATGACCTGGAGAT
  GGCCAAAGACTGGCAGAAAGAGAAGGTGTGCAATAAGATCAACAAGGCAAATGCCGATGTAGAACTGGAC
  AAAGACAGGGACACAGTGTGTGAAACAGTTGACCTAAACAGCCAGGAGACCGTCAAAGTGCAGATACACG
  GCAGAGCCTCAGAATCTATCACTGATGTCCATCTGAATGACCTAGCTACGTCACAGATCCTTCCTTCAAA
  TGAAAGTGTTAGTCCACGGTTATCAGCAAGCCCTCCTAAGCTAGGCAGTCTGTGGCCAGGACTGTCGCCT
  CCACACAAGAAAGCTCAGTCTACATCTGCAAAGAGGAAAAAGCAGCATAAGAAATACAGAAGTGTCATCT
  CCGACATATTCGATGGAACAGTCATTAGCTCGGTACAGTGTCTGACGTGCGATAGGGTGTCTATAACCCT
  CGAGACCTTTCAGGATCTGTCCTTGCCGATTCCTGGCAAGGAGGACCTGGCTAAGCTGCACTCCTCCAGT
  CACCCAACTATAGTCAAAGCAGGGTCATGTGGTGAAGCGTACGCCCCGCAGGGGTGGATAGCTTTCTTCA
  TGGAGTATGTGAAGAGCTGGTTTTGGGGTCCAGTAGTTACCTTGCAAGATTGTCTTGCTGCCTTCTTCGC
  CAGAGATGAACTTAAAGGTGACAACATGTACAGTTGTGAAAAATGCAAAAAGTTGAGGAATGGAGTAAAG
  TTTTGTAAAGTACAGAAGTTTCCTGAGATTTTGTGTATCCACCTTAAAAGATTTCGACATGAACTGATGT
  TTTCCACCAAAATTAGCACCCATGTTTCCTTCCCCCTGGAAGGCCTCGATCTTCAGCCATTTCTTGCAAA
  GGACAGCCCAGCTCAGATTGTGACATATGATCTCCTGTCAGTCATCTGTCACCATGGGACTGCAAGTAGT
  GGGCACTACATTGCCTACTGCCGAAACAATTTAAATAACCTGTGGTATGAGTTTGATGACCAGAGCGTCA
  CTGAAGTTTCAGAGTCCACTGTACAGAATGCCGAGGCCTACGTCCTTTTCTACAGGAAGAGCAGTGAAGA
  GGCACAAAAGGAGAGGCGGAGGATATCAAATTTGTTGAACATCATGGAACCTAGCCTCCTTCAGTTCTAC
  ATATCTCGACAGTGGTTGAATAAATTTAAGACCTTTGCTGAACCTGGCCCTATTTCAAATAATGATTTTC
  TCTGTATCCATGGAGGTATTCCTCCACGAAAAGCGAGTTATATTGAAGACTTAGTTTTGATGCTGCCTCA
  GAACATTTGGGATAACCTCTATAGCAGGTATGGAGGAGGGCCTGCTGTCAACCATCTCTACATCTGCCAC
  ACCTGCCAAATTGAGTTAGAGAAGATTGAAAAACGAAGAAAAACCGAATTGGAAATTTTTATTCGGCTCA
  ACAGAGCATTTCAAGAGGAGGACTCCCCAGCTACTTTTTACTGTATCAGCATGCAGTGGTTTAGAGAATG
  GGAGAGTTTTGTAAAGGGTAAGGATGGAGATCCCCCAGGTCCAATCGACAACACTAAAATTGCGGTTACT
  AAATGTGGCAGTGTGATGCTCAAGCAAGGAGCAGACTCTGGTCAAATTTCAGAAGAAACATGGAATTTCC
  TGCAGTCTATATATGGTGGGGGGCCTGAAGTTATCCTCCGACCTCCAGTTGTTCATGTTGACCCTGATGT
  ACTCCAAGCAGAGGAAAAGATTGAAGTAGAAACTCGCTCTTTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252486
• Insert Size: 2706 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252486.1, NP_001239415.1
• RefSeq Size: 4185 bp
• RefSeq ORF: 2706 bp
• Locus ID: 170822
• UniProt ID: Q8R5K2
• Cytogenetics: 3 H3
• Gene Summary: Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate in-frame splice junction compared to variant 1, resulting in a shorter protein (isoform 3).