C2cd5 (NM_001286578) Mouse Untagged Clone

CAT#: MC228991

C2cd5 (untagged) - Mouse C2 calcium-dependent domain containing 5 (C2cd5), transcript variant 4

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: C2cd5
• Synonyms: 5730419I09Rik; C030008B15Rik; CDP138
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228991 representing NM_001286578
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGTGAATTACAGAGAAAGATTGGCTTGAAAGTCCTCGAGATGAGAGGGAATGCAGTTGTTGGGT
  ACTTGCAGTGCTTTGATCTGGAGGGCGAGTCTGGGTTAGTGGTCCGAGCCATAGGAACGGCGTGCACTCT
  GGATAAACTGAGCAGCCCAGCAGCGTTCCTTCCTGCATGCAGCTCCCCATCCAGAGAACTGAAGGAGATT
  CCCTTCAATGAGGATCCCAACCCCAATACTCACTCATCAGGACCCTCAACCCCTCTGAAAAACCAAACTT
  ATTCCTTTTCACCTTCCAAGTCCTACAGTCGGCAGTCCTCTTCTTCTGACACAGATTTGAGTTTGACACC
  CAAGACTGGAATGGGCAGTGGGAGTGCTGGAAAAGAAGGGGGGCCCTTTAAAGCCCTTTTACGGCAACAG
  ACGCAGTCGGCGTTGGAACAGCGGGAGTTCCCGTTCCTCACCCTGACGGCCTTCCCCCCTGGACTTCTTG
  TGCACGTCGGTGGCGTTGTCAGTGCACGCTCTGTGAAGCTTTTAGATCGGATCCACAACCCTGATGAACC
  AGAAACTCGAGATGCATGGTGGGCAGAAATCAGACAAGAGATAAAGTCACATGCTAAAGCCTTAGGCTGC
  CATGCTGTAGTGGGCTACAGCGAGTCAACCAGCATCTGTGAAGAAGTCTGTATTTTATCAGCGTCTGGCA
  CGGCAGCTGTGCTGAACCCTCGGTTTCTGCAGGAAGGCACTGTGGAGGGCTGTTTGGAACAAAGGATTGA
  AGAGAATTTACCGGTGGGCTGCGGATTCTGCCATATACCATATGATGAACTGAACATGCCGTTTCCCGCT
  CATCTCACATATTGCTATAACTGCAGGAAGCAAAAAGTCCCTGATGTTCTCTTCACAACTATCGATCTGC
  CCACAGACGCAGTGGTTGTTGGTAAAGGTTGTCTTATTCAGGCAAGGTTATGTCGATTAAAAAAGAAAGC
  ACAGGCAGAAGCCAATGCCACAGCTATCAGTAATCTCTTGCCGTTTATGGAATATGAAGTACACACTCAA
  CTGATGAACAAGCTCAAACTCAAAGGAATGAATGCTTTGTTTGGACTGAGAATTCAGATCACAGTGGGCG
  AGACCATGCTGATGGGCTTAGCGTCGGCCACAGGAGTCTACTTAGCAGCCTTACCCACACCCGGTGGAAT
  CCAGATCGCTGGGAAGACCCCTAATGATGGCTCCTATGAACAGCACATCTCCCACATGCAGAAGAGAATA
  AACGACACCATTGCAAAAAACAAAGAGCTGTATGAGATCACCCCCCCGGAGGTGTCTGAGGAGATGATAG
  GATCCCCCATCCCAGAACCCAGACAGCGCTCCAGACTTCTAAGATCACAGTCGGAAAGTTCTGATGAAGT
  TACAGAGTTAGATCTTTCACACGGGAAAAAGGATGCTTTTGTTTTAGAGATCGATGACACCGATGCCATG
  GAAGATGTGCACTCCCTCCTTACTGACGCCCCGCCCCCTTCAGGTTTTTATAGTTGTAATACAGAAATTA
  TGCCTGGCATAAATAACTGGACATCAGAAATACAGATGTTCACGTCAGTAAGAGTGGTCAGACTAAGTAG
  CCTCAACTTGACTAACCAAGCTCTCAATAAGAACTTCAACGGCCTATGTGAGAACTTGCTCAAGAGCCTG
  TATTTCAAACTTCGGTCCATGACCCCCTGCTGCCTCTGCCATGTGAACTTTACAGTGTCTCTGCCTGAAG
  ATGAGTTAATCCAGGTCACAGTCACAGCAGTTGCAATAACTTTTGATAAAAACCAGGCCTTACAGACCAC
  AAAACCCCACGTTGAAAAGTCACTACAAAGAGCCTCCACAGATAACGAAGAACTGCTGCAGTTCCCACTG
  GAGCTCTGCTCAGACTCACTGCCTCCTCATCCTTTCCCAGCAGCTAAAGAACACCTGGAGAGTGCGAACT
  CTAACTCAGGTATACCAGCTGCACAGAGAGCTGTGACTGTTGAAAAAGCAAGTGCCATGGGTGATGGAAA
  TTTCCGGAATCGCTCTGCTCCACCTTGTGCAAGTCCCACAGTTGGGGTTGTTAAGATGACGCCGCTTTCT
  TTCATCCCTGGAGCAAAGATAACCAAGTACCTCGGCATCATTAACATGTTTTTCATTCGGGAAACCACTT
  CTCTCCGCGAGGAAGGCGGAGTCAGTGGTTTTCTCCACGCTTTCATTGCTGAAGTGTTTGCCATGGTCCG
  AGCTCACGTGGCAGCGCTGGGCGGGAATGCTGTTGTGTCCTACATCATGAAGCAGTGTGTCTTCATGGAG
  AACCCCAGTAAGAACCAGGCACAGTGTCTCATAAATGTCAGTGGGGACGCGGTGGTGTTTGTTCGTGACT
  CCGACTTAGAGGTCATGTCATCTCAGCAGCCTGCTGCCAACTGCCAGCCCTCATGTACCGGAGAAGTTAC
  AACTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286578
• Insert Size: 2457 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286578.1, NP_001273507.1
• RefSeq Size: 4216 bp
• RefSeq ORF: 2457 bp
• Locus ID: 74741
• UniProt ID: Q7TPS5
• Cytogenetics: 6 G3
• Gene Summary: Required for insulin-stimulated glucose transport and glucose transporter SLC2A4/GLUT4 translocation from intracellular glucose storage vesicle (GSV) to the plasma membrane (PM) in adipocytes. Binds phospholipid membranes in a calcium-dependent manner and is necessary for the optimal membrane fusion between SLC2A4/GLUT4 GSV and the PM.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) lacks an alternate exon in the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 4, which is shorter at the N-terminus, compared to isoform 1.