Thsd1 (NM_001205253) Mouse Untagged Clone

CAT#: MC228940

Thsd1 (untagged) - Mouse thrombospondin, type I, domain 1 (Thsd1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Thsd1
• Synonyms: 4833423O18Rik; Tmtsp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228940 representing NM_001205253
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTGGGAGGCCAACACCAATCGGACTCTTACCACTAAGTACCTCCTGACCAACCAGGCCCAAGGAA
  CACTCCAGTTTGAATGTTTCTACTTCAAAGAGGCTGGTGACTACTGGTTTGTAATGATCCCGGAAGTGAC
  AGACAATGGCACGCAAGTTCCACTCTGGGAGAAAAGTGCCTTTCTGAAGGTAGAATGGCCTGTCTTTCAC
  ATTGATTTAAATAGGACAGCCAAGGCAGCAGAAGGCACCTTTCAAGTGGGTGTTTTTACCACCCAACCGC
  TCTGCCTGTTTCCCGTGGACAAGCCAGACATGCTGGTGGATGTTATTTTCACTGACCGTCTTCCGGAGGC
  AAGAGCAAGTTTGGGACAGCCGCTGGAGATCAGAGCCAGCAAAAGGACAAAACTCACTCAAGGTCAGTGG
  GTCGAGTTTGGCTGTGCACCGGTAGGGGTGGAAGCCTACGTTACAGTCATGCTGAGGCTGTTGGGTCAAG
  ACTCAGTCATTGCTTCTACGGGACCTATTGACCTGGCTCAAAAATTCGGATACAAATTGATGATGGCACC
  GGAAGTCACGTGTGAGTCTGTGCTGGAGGTGATGGTACTGCCACCTCCTTGTGTCTTCGTCCAAGGAGTC
  CTGGCTGTTTACAAAGAAGCCCCCAAACGCCCGGAGGAGAGGACTTTCCAGGTGGCTGAAAACAGACTGC
  CCCTGGGAGAGAGGAGAACGGTGTTCAACTGCACTTTATTTGATGTAGGGAAGAACAAATACTGTTTTAA
  CTTTGGAATTGTGAAGAAAGGCCATTTTTCTGCAAAGGAATGCATGCTAATTCAGAGAAATATAGAAACT
  TGGGGACCATGGCAGCCGTGGAGCCCGTGTAGCACCACGTGCGGGGATGCTGTCCGAGAGCGTCGCCGCC
  TGTGTGTCACTTCTTTCCCCTCCAGACCCAGCTGCTCTGGAATGTCCTCAGAGACCTCTCCATGCTCCCT
  GGAGGAGTGTGCTGTTTTCCGGCCACCAGGCCCATCGCCTGTTTCACCCCAGGACCCTGTGAAGTCCAAC
  AACGTGGTGACCGTCACAGGGATCTCCCTGTGCCTGTTCATCATCTTTGCCACGGTGCTCATCACTCTCT
  GGAGGAGGTTTGGCCGAGCCCCCAAATGCAGCACGCCCGTTCGCCACAACTCCATCCATTCCCCTGGCTT
  CCGGAAGAACTCCGATGAAGAGAACATCTGCGAGCTGAGTGAGCCTCGCGGAAGCTTCTCGGATGCCGGT
  GACGGACCTAGGGGAAGCCCAGGGGACACGGGCATCCCATTGACTTACAGGTGCAGTGCATCAGCGCCTC
  CTGAGGATGAGGCCTCGGGCAGTGAGAGCTTCCAGTCCAACGCTCAGAAGATCATCCCGCCCTTGTTTAG
  CTACCGCCTTGCCCAGCAGCAGCTGAAGGAGATGAAGAAGAAAGGGCTGACCGAGACCACCAAAGTGTAC
  CACGTATCTCAAAGCCCCCTGACAGACACTGTAGTGGATGCCACGGCCAGCCCTCCCTTAGACCTGGAAT
  GCCCCGAAGAGGCTGCAGCAAGCAAGTTCCGAATCAAATCTCCATTTCTGGACCAGCCTGGGGCAGGTAC
  CGGGGAAAGGCCTCCCTCCAGGCTGGATGGCATCGTGCCTCCTCCTGGCTGTGCGGTCAGTCCCAGCCAG
  ACCCTGATCCGAAAGTCACAGATAAGGTCGACCGGTGGCAGAGATGGCTCATCGGAGAGGTGCCACTCCA
  GAAGTTCCCTCTTCAGGAGGACTGCTAGTTTTCATGAAACCAAGCAGTCTCGCCCTTTCCGGGAGAGGAG
  TTTGTCAGCCCTGACTCCCCGCCAGGTCCCCGCCTACAGTTCCAGGATGCGGACCTGGGACCAGATGGAG
  GATAGATGTCGGCCTCCCAGTCGAAGTACCCACCTGCTTCCAGAGAGACCAGAGCACTTCCAAGGGGCAG
  GTCGGACCAGCAGTCCTTTGGGTCCACTCTCCAAATCCTACACTGTGGGGCATCCCAGGAGGAAACCGGA
  CCCAGGGGATCGTCAGGCCGGATTGGTGGCAGGAGCTGAGAAAATGGAGCCTCACCGAGCTCACAGGGGA
  CCGTCCCCCAGTCACAGGAGTGCCTCAAGGAAGCAGTCTTCCCCCATTTTCCTCAAAGATAGCTACCAGA
  AAGTCAGTCAGCTTAGCCCTTCTCACTTCAGAAAAGATAAATGCCAGAGCTTCCCCATCCACCCCGAGTT
  CGCCTTCTATGACAATACCTCTTTCCGCCTCACCGAGGCTGAGCAGAGAATGCTGGACCTCCCAGGATAC
  TTCGGCTCCAACGAAGAGGACGAAACCACAAGTACACTCAGTGTGGAGAAGTTAGTGATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205253
• Insert Size: 2373 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205253.1, NP_001192182.1
• RefSeq Size: 4289 bp
• RefSeq ORF: 2373 bp
• Locus ID: 56229
• Cytogenetics: 8 A2
• Gene Summary: Is a positive regulator of nascent focal adhesion assembly, involved in the modulation of endothelial cell attachment to the extracellular matrix.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks an exon in the 5' coding region and uses a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus and lacks a predicted signal peptide, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.