Usp1 (NM_001301414) Mouse Untagged Clone

CAT#: MC228929

Usp1 (untagged) - Mouse ubiquitin specific peptidase 1 (Usp1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Usp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228929 representing NM_001301414
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGGCGTCATACCTAGTGAAAGTAATGGGCTTTCAAGAGGCAGTCCATCCAAGAAAAACAGACTGT
  CTCTGAAGTTTTTTCAGAAAAAGGAAACTAAAAGAGCCTTAGACTTTACTGATTCTCAAGAAAATGAAGA
  AAAAACTTCTGAATATAGAGGATCTGAGATTGACCAAGTTGTTCCTGCTGCACAGTCCTCCCCAGTAAGC
  TGCGAGAAGAGAGAGAACTTGTTACCATTTGTGGGATTGAATAACCTTGGCAACACTTGTTATCTGAATA
  GTATTCTTCAGGTGTTATACTTTTGTCCTGGCTTTAAGACTGGAGTGAAGCACTTATTTAATATTATTTC
  AAGGAAGAAAGAAGCTCTGAAGGATGACTCTAATCAGAAGGATAAGGGAAGCTGCAAAGAAGAGTCTTTG
  GCAAGTTATGAGCTTATATGTAGTTTACAGTCCTTAATAATTTCAGTTGAACAGCTTCAGGCTAGTTTTC
  TCTTAAATCCAGAGAAATATACAGATGAACTTGCTACACAGCCAAGGCGACTGCTGAACACACTCAGGGA
  ACTCAACCCTATGTATGAAGGATTTCTACAGCATGATGCACAGGAAGTGCTACAGTGTATTCTGGGAAAC
  ATTCAAGAAACATGCCAACTCCTAAAAAAAGAAGAAATAAAAAACCTGGCTGAATTATCCGGCAAGGTTG
  AAGAACAATCTCTTCAGAAAGAGGAAACAGGTGGAATTACCAGCACAGAGATTGACAGTATGAGGAATAC
  GGAGGACGTCAAAGAGCAGCTCCCCAAAGGGAACTGGAAAAGAAAAAGTGACAGTGAGTCGAGTAACGTG
  AAGAAAAAGGTTAAACTGTCCAGGGAATCCCAGCCATTGGAAGAAAACCAGAGACAAACCAGATCCAAGA
  GAAAAGCTATCGGAGACACACTAGAGGCTGCTCCTAAGATCATCCCCAAGTGTGTTTCTGAAAGTGAGAG
  TGCAAAGCCCTCACAGAAGAAATCAAAAGTTAAAATAAATTGGTTAAAGCCATCCACTAAGCAACCCAGC
  ATTCTTTCCAAGTTCTGTAGTCTTGGAAAAATAACAACTAACCAGCGCTCCAAAGGACAACCTAAGGAAA
  AGGAGGGTGATGTTGAAGAGGACTTGGAGAAGTACGGAAGTGACCACACAGCTAATGGTGGACCCGAATC
  TCCAGGAAGTAGCGTCACACCTGTGGACAGCAGTGAAGCCAAGTCCGGAAACAAAGGTGCTGAACAGATT
  GGCTTTGAGCTAGTAGAGAAACTGTTTCAAGGTCAGCTCGTGTTAAGGACTCGGTGTTTGGAATGTGAAA
  GCTTAACAGAAAGAAGAGAAGATTTCCAGGATATTAGTGTTCCAGTGCAAGAAGATGAGCTTTCCAAGGT
  CGAGGAGAGCTCTGAAATTTCTCCAGAGCCAAAAACAGAAATGAAGACCTTGAGATGGGCAATTTCACAG
  TTTGCTTCAGTGGAGAGAATTGTAGGAGAGGATAAATACTTCTGTGAAAATTGCCATCATTATACGGAAG
  CAGAGCGAAGTCTCCTGTTTGATAAAATGCCTGAAGTTATCACTATTCATCTGAAGTGCTTTGCTGCTAG
  TGGCTTGGAGTTTGACTGTTACGGTGGTGGACTTTCCAAGATCAACACTCCTTTATTGACACCTCTTAAA
  CTGTCACTAGAAGAATGGAGCACAAAGCCGACGAATGACAGCTATGGATTATTTGCCGTTGTGATGCACA
  GTGGCATTACTATTAGTAGTGGGCACTATACCGCATCTGTTAAAGTCACTGACCTTAACAGTCTAGAGCT
  CGATGAGGGGAATTTTGTGGTTGACCAGATGTGTGAACTAGGTAAGCCAGAGCCACTGACCGAGGAGCAA
  GCAAGGGGGACGGCTGGGAACTACGACGATGAAGTATCAATTAGAGTTGGTGGAAATGCCCAGCCAAGTA
  AAGTTTTGAACAAAAAAAATGTAGAAGGTATTGGACTTCTCGGTGGACAAAAGAGCAAAGCAGATTATGA
  GCTGTACAACAAAGCATCTAATCCTGATAAGGTGGTTGGGACACCATTCACCGACAATAGAAATTCTGAA
  ACTAATGATACTACTAATGGGACCCACGAATCTGACAGGAACAAGGAATCCAGTGACCAAACAGGCGTTA
  ACATGAACGGACTTGAGAACAAAATTTCCTATGTAGTGCAAAGCTTAAAGGAGTACGAGGGGAAGTGGTT
  GCTCTTTGACGATTCTGAGGTAAAAGTGACAGAAGAGAAAGACTTTCTGAATTCTCTCTCCCCTTCTACA
  TCCCCTACGTCTACTCCCTACTTGCTATTCTATAAAAAATTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301414
• Insert Size: 2355 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301414.1, NP_001288343.1
• RefSeq Size: 3608 bp
• RefSeq ORF: 2355 bp
• Locus ID: 230484
• UniProt ID: Q8BJQ2
• Cytogenetics: 4 C6
• Gene Summary: This gene encodes a member of the ubiquitin-specific peptidase family. The encoded protein acts as a catalytic subunit in a heterodimeric deubiquitinating enzyme complex that deubiquitinates Fanconi anemia, complementation group D2, and plays a role in homologous recombination-mediated DNA repair. Disruption of this gene is associated with a Fanconi anemia-like phenotype and genomic instability. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 3, 12, and 15. [provided by RefSeq, Aug 2014]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.