E4f1 (NM_001301784) Mouse Untagged Clone

CAT#: MC228926

E4f1 (untagged) - Mouse E4F transcription factor 1 (E4f1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: E4f1
• Synonyms: p120E4F; phi-AP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228926 representing NM_001301784
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGCGCGATGGCAGTGCGGGTGACGGCCGCTCATACGGCAGAAGCCCGGGCCGAAGCCGGGCGTG
  AAGCGGGCGAGGGCGGGGTTGCGGCGGCGGCGGCCTTGTCCTCCGGCGGCTTCCTTGGGCTCCCAGCGCC
  CTTCAGCGAGGAAGATGAGGACGATGTGCACAGATGTGGCCGCTGCCAAGTGGAATTCACTGCCTTGGAG
  GACTTTGTCCAGCACAAGATTCAGAAGACCTGCCATCGGGCCCCGCAGGAGGCCCTGCCCACCACCCCTG
  CTGCCACTGCACTGCTGGACCAGGAGGTGGTGCCCACAGCAGCAGAGGGAGGTCCAGATGAACCCATCAC
  TGTGGCTCACATCGTGGTGGAGGCCACCTCTCTAGCAGAAGACATCAGCCACGCTCCTGACCTTGTTGGA
  AGTGGGCACATCAAAGAGGTCATTGTGGCTGCTGAGGCAGAGCCAGGGGATGTCGAGATGGCAGAGGCTC
  CAGGCAGTCCTAATCATCAGGAACTTGGGCTTCTTGGGGAGGGCGAGCAGGCCCATGTCAAGCTGCTGGT
  GAACAAGGAAGGCCGCTACGTGTGCATGCTATGTCACAAGACCTTCAAAACGGGCAGCATCCTCAAGGCC
  CACATGGTAACGCACAGCAGCCGCAAGGACCACGAGTGCAAGCTCTGTGGGGCCTCTTTTCGGACCAAGG
  GCTCTCTCATCCGGCACCACCGACGGCACACTGATGAGCGCCCCTACAAATGTGCCAAGTGTGGAAAGAG
  TTTCCGAGAGTCAGGCGCACTGACTCGGCACCTCAAATCTCTCACTCCATGCACAGAAAAGATCCGCTTC
  AGCATAAGCAAGGACACAGCTGTGGGCAAAGAGGAAGTGCCTGCAGGGTCCAGTGCCTCCACTGTGGGGA
  CGGTGACATCATCAGTGGCAGGAGACCCCATGGAGACATCACCTGTGATTCACCTGGTGACAGATGCCAA
  GGGTACTGTCATCCATGAAGTCCACGTCCAGATGCAGGAGCTTCCCCTGGGCATGAAAGCCCTGACCCCA
  GAGTCCCCAGACTCGGAGGAGCTCCCCTGTTCCAGTGAGAACAGCCGTGAGAACCTGCTACATCAGGCCA
  TGCAGAATTCTGGCATCGTCCTTGAGAGGGTTGCTGGAGAGGAGAGTGCTCTGGAGCCAGCCCCTCCCTC
  TGGGTCCAGTCCCCAGTGCCTGGGAGATGGGTCCCCTGAACTGCCTCTGCTGAAGGTGGAGCAGATAGAG
  ACACAGGTGGCCAGTGAGGCCGCCACCGTGCCCAGGACCCACCCGTGCCCTCAGTGCAGTGAGACTTTCC
  CAACAGCAGCCACGCTGGAGGCCCACAAGAGAGGTCACATAGCGCCGAGGCCGTTCACCTGCACACAGTG
  TGGCAAGGCCTTCCCCAAAGCCTACCTGCTCAAGAAGCACCAGGAGGTGCACGTGCACGAGCGCCGCTTC
  CGTTGTGGAGACTGTGGGAAGCTTTACAAGACCATCGCTCATGTGCGGGGCCACCGGCGTGTTCACTCAG
  ACGAGAGGCCTTTCCCTTGTCCCCAGTGCGGCAAGCGTTACAAAACCAAGAATGCCCAGCAAGTACACTT
  CCGGACACACCTGGAAGAAAAGCCCCACGTGTGCCAGTTCTGCAGCCGAGGCTTCCGGGAGAAGGGCTCT
  CTGGTGCGGCATGTGAGGCACCACACAGGCGAGAAACCTTTCAAGTGCTACAAGTGTGGCCGTGGCTTCG
  CGGAGCATGGCACACTCAACCGGCACCTGCGCACTAAAGGGGGCTGCCTGCTAGAAGTGGAGGAGTTGCT
  GGTGTCTGAGGAGAGCCCTTCTGCGGCTGCCACTGTGCTTGCAGAAGACCCCCACACCGTGCTGGTGGAG
  TTCTCGTCTGTGGTAGCTGATACCCAAGAGTACATTATTGAGGCCACTGCAGATGACACAGAGACCAGTG
  AAGCCACCGAGATCATTGAGGGCACGCAGACAGAGGTGGACAGTCACATCATGAAGGTGGTCCAGCAGAT
  CGTGCACCAGGCTGGTGCTGGGCACCAGATCATCGTGCAGAATGTCACCATGGACCAGGAGACAGCACTG
  GGTTCGGAGGCAACTGCTGCAGACACGATCACCATTGCCACTCCTGAGAGTCTTACTGAGCAGGTGGCCA
  TGACACTGGCTTCAGCGATCAGTGAGGGTACTGTGCTCACAGCCCGGGCAGGTCCAAACAGTACTGAACA
  GGCCACTGTGACAATGGTGTCATCAGAGGACATAGAGATCCTTGAGCACGGAGGAGAGCTGGTCATTGCT
  TCACCAGAGGGCCAGCTTGAGGTACAGACGGTCATCGTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001301784
• Insert Size: 2352 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301784.1, NP_001288713.1
• RefSeq Size: 2669 bp
• RefSeq ORF: 2352 bp
• Locus ID: 13560
• UniProt ID: Q8CCE9
• Cytogenetics: 17 12.39 cM
• Gene Summary: This gene encodes a member of the GLI-Kruppel zinc finger family. The encoded protein is likely to be multi-functional, with both adenovirus E1A-regulated transcription factor and ubiquitin E3 ligase activities, including roles in cell cycle regulation and the ubiquitination of p53. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).