Plcd1 (NM_001293648) Mouse Untagged Clone

CAT#: MC228924

Plcd1 (untagged) - Mouse phospholipase C, delta 1 (Plcd1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Plcd1
• Synonyms: AW212592; C79986
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228924 representing NM_001293648
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTGTCTAGGGGTCCGGAGTTGCAGACGGGGCAGCGTGAACCGCTCCCGGGAGCTCTACCTGCAGG
  AGCAGAGTCGCAAAGTAGCGGCGCTCAATGGACAGAGACTGGGGCTGCAGGATGACCCGGACCTTCAGGC
  CCTCCTGAAGGGCAGCCAGCTTCTGAAGGTGAAGTCCAGCTCGTGGCGTAGAGAACGCTTCTACAAGCTA
  CAGGAGGACTGCAAGACCATCTGGCAGGAATCTCGCAAGGTCATGAGGTCCCCGGAGTCGCAGCTGTTCT
  CCATCGAGGACATTCAGGAGGTGCGGATGGGACACCGCACAGAAGGCCTGGAGAAATTTGCCAGAGACAT
  ACCCGAGGACCGATGCTTCTCCATCGTCTTCAAGGACCAGCGCAATACCCTAGACCTCATCGCCCCGTCT
  CCAGCTGACGTCCAGCATTGGGTGCAGGGCCTGCGCAAGATCATCGACCGCTCGGGCTCCATGGACCAGC
  GGCAGAAGCTGCAGCACTGGATTCACTCCTGCTTGCGAAAGGCTGATAAAAACAAGGATAACAAGATGAA
  CTTCAAGGAGGTGAAGGACTTCCTGAAGGAGCTCAACGTACAGGTGGATGACAGCTACGCCAGGAAGATC
  TTCAGGGAATGTGACCACTCCCAGACAGACTCGCTGGAGGATGAGGAGATTGAGACCTTCTACAGGATGC
  TGACCCAGCGGGCAGAGATCGACCGTGCCTTTGCAGAGGCCGCAGGTTCTGCGGAGACCCTGTCGGTGGA
  GAAGCTAGTGACGTTCCTGCAGCATCAGCAGCGGGAGGAGGAGGCGGGGCCAGCCTTGGCCCTCTCTCTC
  ATTGAGCGCTACGAGCCCAGTGAGACTGCCAAAGCCCAGCGGCAGATGACCAAGGATGGCTTCCTCATGT
  ACCTGCTGTCTGCTGACGGTAACGCCTTCAGCCTGGCGCACCGCCGTGTCTACCAGGACATGAACCAGCC
  GCTCAGCCACTACTTAGTGTCTTCTTCCCACAACACCTACCTACTGGAGGACCAGCTCACAGGGCCCAGC
  AGCACGGAGGCCTACATCCGGGCTCTGTGCAAAGGCTGCCGGTGTCTGGAGCTGGACTGCTGGGACGGCC
  CCAACCAGGAACCCATCATCTACCATGGCTACACTTTTACCTCTAAGATACTTTTCTGCGACGTGCTCAG
  GGCCATCCGGGACTACGCCTTCAAGGCATCCCCCTACCCAGTTATCCTGTCCCTGGAGAACCACTGCAGC
  CTGGAGCAACAACGAGTCATGGCTCATCACCTGAGGGCCATCCTGGGGCCCATGCTGTTGGACCAGCCGC
  TGGATGGGGTTACCACGAGCCTGCCTTCGCCTGAGCAACTGAAGGAAAAGATTCTGTTGAAAGGGAAGAA
  ACTGGGCGGGCTGCTACCTGCTGGAGGGGAAAACGGGCCTGAAGCCACCGATGTGTCTGACGAAGATGAG
  GCTGCTGAAATGGAAGATGAGGCCGTGCGCAGCCAAGTGCAACACAAGCCCAAGGAGGATAAGCTAAAGC
  TGGTGCCGGAGCTCTCCGACATGGTCATTTACTGCAAGAGTGTCCACTTTGGTGGCTTCTCCAGTCCTAG
  CACCTCTGGGCAGGCATTCTATGAGATGGCTTCCTTCTCCGAGAGCCGTGCCCTGCGGCTGCTGCAAGAA
  TCAGGAAATAGTTTTGTCCGCCATAACGTGGGCCATCTGAGCAGGATCTACCCAGCTGGGTGGAGGACAG
  ATTCCTCCAACTACAGTCCCGTGGAGATGTGGAATGGAGGCTGCCAGATTGTGGCTCTGAACTTCCAAAC
  CCCTGGGCCAGAAATGGATGTTTACCTTGGCTGCTTCCAGGACAATGGAGGTTGTGGGTATGTGCTGAAA
  CCGGCCTTCCTGAGAGACCCTGACACCACCTTCAACTCACGGGCCCTGACTCAGGGGCCCTGGTGGGCCC
  CGAAGAAGCTCCGAGTCTGGATCATCTCTGGACAGCAGCTGCCGAAGGTCAATAAGAATAAGAATTCTAT
  CGTGGACCCCAAGGTGATAGTGGAGATCCACGGTGTGGGCCAGGATGTGGCCAGCCGTCAGACAGCAGTT
  ATTACCAATAATGGTTTCAACCCACGGTGGGACACGGAGTTTGAGTTTGTGGTAGCAGTGCCTGACCTTG
  CCCTTGTACGTTTCATGGTAGAGGATTATGACTCCTCCTCTAAGAACGACTTTATCGGCCAGAGTACTAT
  CCCCTGGAACAGCCTCAAGCAGGGGTACCGCCATGTCCACCTCTTGTCTAAGAATGGAGACCTGCATCCA
  TCAGCCACTCTCTTTGTGAAGATCTCCATCCAGGACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001293648
• Insert Size: 2349 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293648.1, NP_001280577.1
• RefSeq Size: 2706 bp
• RefSeq ORF: 2349 bp
• Locus ID: 18799
• UniProt ID: Q8R3B1
• Cytogenetics: 9 F3
• Gene Summary: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Essential for trophoblast and placental development.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.