Gfm2 (NM_001271465) Mouse Untagged Clone

CAT#: MC228917

Gfm2 (untagged) - Mouse G elongation factor, mitochondrial 2 (Gfm2), transcript variant 5

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gfm2
• Synonyms: 6530419G12Rik; A930009M04Rik; EF-G2mt; EFG2; MST027; RRF2mt
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228917 representing NM_001271465
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCACCAAATGGAGGATTTTTGCAGTGAATCATCAGAGAACGTTCAGTGTGCACCTTAATACCATGT
  GTTACTGCAAAATAAAAGCAAATTTAAAAAGATTAAAGACTCAGCTGCCTCTTACAAGAAACTACAGTTC
  TGCACCAGCAGGAAGTGATGTCAAGTCTCTTCATTCCGTCATTAACCCTCCTGTGGCCAAAATTCGTAAT
  ATTGGAATTATGGCACATATTGATGCAGGCAAAACCACCACCACAGAGAGAATCCTGTACTATTCTGGAT
  ACACAAGATCATTGGGAGATGTCGATGATGGAGACACAGTGACGGATTTCATGGCTCAAGAGCGAGAAAG
  GGGCATTACCATCCAGTCAGCAGCTGTGACTCTCGACTGGAAAGGGTATAGAGTCAATCTGATTGATACT
  CCAGGTCACGTAGACTTCACTCTGGAGGTCGAGCGCTGCTTGAGGGTGTTGGACGGTGCGGTGGCCGTGT
  TTGACGCCTCTGCTGGTGTAGAGGCCCAGACTCTCACTGTATGGAGGCAAGCTGACAAACACAAAATACC
  CCGGATCTGCTTTTTAAACAAGATGGACAAGACTGGAGCAAGTTTTAACTATGCGGTTGAAAGCATCCGG
  GAGAAGTTAAAGGCAAAGCCCCTGATTTTACAGCTGCCAATTGGTGAAGCCAGAACTTTCCAAGGAGTGG
  TGGATGTCGTAAACAAAGAAAAACTCCTTTGGAATTCTAACTCTGACGATGGAAAAGACTTCGAGAGGAT
  GCCCCTCTCAGAAGCGAGTGATCGGGAACTGCTAAAGGAGACAATTGAAGCAAGGAACTCCTTAATTGAA
  CAAGTTGCAGACTTAGATGATGAGTTTGCTGACTTGGTTTTAGGTGAATTTAGTGAAAACTTTGATTTAG
  TACCAGCTGAAAAGTTACAGGCTGCAGTACACAGGGTGACACTGGCACAGGCTGCAGTGCCTGTGCTGTG
  TGGGAGTGCCTTGAAGAACAAGGGCGTGCAGCCCCTGCTGGATGCGGTGACCACTTACCTGCCGTCACCC
  GAGGAGCGCGAGGACCGCTTTCTGCAGTGGTATGAGGGTGACTTGTGTGCACTAGCGTTTAAAGTACTAC
  ATGACAAACAGCGAGGACCCCTGGTTTTCCTGCGCATTTACTCGGGAACACTGACGCCTCAGCTGGCTGT
  CCACAACATTAATAGAAACTGCACGGAAAGAATGAGTCGTCTGCTTTTGCCATTTGCTGACCAACATGTA
  GAAATCCCTTCCCTGACTGCTGGAAACATTGCTTTGACAGTTGGGCTTAAACAAACTGCCACTGGAGACA
  CCATAGTCTCGTCCAAGTCCAGCGCATTAGCTGCAGCTCGCAGAGCCGGACGAGGAGAAAGGGAGCATGG
  AAAGAAGAGAGAGGCCGAGAGTCTGCTGTTGGCCGGGGTGGAGGTCCCAGAGCCTGTCTTCTTCTGCACC
  ATAGAGCCCCCGTCAGTCGCTAAGCAGCCAGATTTAGATCATGCCTTGGAACGCCTTCAGCGCGAAGATC
  CCAGTTTGAAAGTGAAACTCGATCCTGACTCTGGACAAACTGTCCTGTGTGGAATGGGGGAGTTGCATAT
  TGAGATTATTCATGACCGTATCAAGAGGGAGTATGGCCTAGAGACCTATCTAGGGCCTCTGCAGGTGGCA
  TACCGGGAAACCATCTTAAACTCAGTTCGCGCCACAGACACCCTCGATAGGGTGCTTGGAGACAAGCGGC
  ACTTGGTGAGTGCGGAGCTGGAAGTCCGGCCAGCAGAAGAGCCCTGTGCTGTGGCCAAGATCGAGTATGC
  TGACTGTGTGGGTGAAGACCTGCTGCAGGCCTCTCGGGAGGCCATTGAAAGTGCCGTCCACAGTGCATGC
  CTCCAGGGACCTCTGCTCGGATCCCCAGTCCAGGATGTGGCAATGACGTTGCACTCACTGATGATTCACC
  CTGGCACCTCCACAACTATGGTCACTGCCTGCATCTCACGGTGTATGCAGAAGGCCCTGAAGAAAGCTGA
  CAAACAGGTGCTGGAACCTCTGATGAGTCTCGAGGTCACAGTGAGCAGGGAGTACCTCAGCCCTGTCCTG
  GCAGACTTGGCACAAAGAAGAGGGAACATTCAGGAAATCCAGACTCGCCAAGACAACAGAGTTGTTCTAG
  GATTCGTTCCTCTAGCAGAGATTATGGGATATTCAACTGTGCTTCGAACTCTGACATCTGGCTCAGCTAC
  TTTTGCCTTAGAACTGTCTACTTATCAAGCCATGAGTCCTCAAGACCAGAGTGCTCTACTCAACCAGAGA
  AGTGGCTTGGCCCATGTGCTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271465
• Insert Size: 2334 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271465.1, NP_001258394.1
• RefSeq Size: 3192 bp
• RefSeq ORF: 2334 bp
• Locus ID: 320806
• UniProt ID: Q8R2Q4
• Cytogenetics: 13 D1
• Gene Summary: Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Acts in collaboration with MRRF. GTP hydrolysis follows the ribosome disassembly and probably occurs on the ribosome large subunit. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (4) is shorter than isoform 1.