Tdrd3 (NM_001253755) Mouse Untagged Clone

CAT#: MC228751

Tdrd3 (untagged) - Mouse tudor domain containing 3 (Tdrd3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tdrd3
• Synonyms: 4732418C03Rik; 6720468N18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228751 representing NM_001253755
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGGTGTCCGGCGCCGCGTTGTCCCAGGCGGGTTGGTACCTTTCAGATGAAGGCGTTGAAGCCT
  GCACAAGCTCTCCTGGGAAAGGCAGCATAAATGACATCATCCTGATTGCTCTCAATACAGATTTGAGAAC
  AATTGGCAAGAAGTTCCTTCCCAGTGACATCAACGGTGGAAAGGTAGAGAAGCTTGAAGGTCCATGTGTC
  TTACAAATTCAGAAGGTCCGCAATGTTGCTGCGCCAAAGGATAACGAGGAATCGCAAGCTGCGCCAAGGA
  TGCTCCGCGTGCAGATGACGGATGGGCATACAAGTTGTACGGCAGTAGAATTCAGTTATATTTCAAAAAT
  AAGCCTGAACACACCACCTGGCACAAAAGTGAAGCTCTCTGGCACTGTGGACATAAAGAACGGATTCCTG
  CTCTTAAGTGACTCTAACACCACAGTTCTTGGTGGTGAAGTGGAACACCTAATTGACAAGTGGGCTTTAC
  AGAGAAGCTTATTAAAGCATAACAGAAGCAATATTGGAGCTGAAGGTGGCCCACCTCCTTTTCTACCTTT
  TGGGCAGAAGTGTGCATCTAACGTCCAAGTGGATAGCAGAGAGCTTGATCGAAGAAAGACATTACAAGTT
  TCCCTGCCTGCCAAGCCTGCAAATGATAATGATGAATTTGAAAAGCAAAGAACTGCTGCCATTGCAGAGG
  TTGCAAAGAGCAAAGAGACCAAGACATTTGGAGGAGGTGGAGGTGGTGCCAGAAGTAATCTCAATATTGG
  TGCTGCTGGTCACCGAAATAGGGAGGTTTTACAGAAGGAAAAGGCTTCCAAGTCAGAGAGCAAGAACGAA
  GGTGTCTATAGAGAATTGGTTGATGAGAAAGCCCTGAAACACATAACAGAAATGGGCTTCAGTAAGGAGG
  CCTCAAGGCAAGCGCTGATGGATAATGCCAACAACTTAGAAGCAGCACTGAATGTCCTTCTGAACAGCAG
  TAAACAGAAGCCTGCTGTGGGCCCTCCTGCTAGAGGAAGAGGGAAAGGCAGGGGTCGAGGACGATCAGAA
  GATGAGGAAGACCTGGGAACCGCAAGACCGTCAGCACCAAGCACGTTATTTGATTTCTTGGAGTCTAAAA
  TGGGAACTCTGAATGTGGAAGAACCTAAGTCACAGCCACAGCACCTTCATCAAGGACAGCACAGAGGATG
  GAATGCCGAGCAAAATGGGATGAAGGACGGCACTCAGTCAAGACATCTTCCTCGAAACGATACCAGGCAG
  CCAAGAAATGAGAGGCCACCTCGCTTTCAAAAAGACACCCCAACTTCCAAGTCAACTGTAGAAAACAGCG
  TATTATCTAGAAATAGAGGCTCCGAAAGGCCGAGTAGTTCCTCAGGTTCTGATGTATGGGCAGAAGAGAG
  GATCAAGTGTGACAGGCCATATTCTAGGTATGACAGAACTAAAGACGCTTCACATCCTCTAGGCCTTCAG
  CACAACGATGGTGCTTTTAAAAAGAGAGAGAACTCTATGCAAAACAGACCAGGAAGAGGCCCTTTGTATG
  CAGAGGCCAAAGAAAACCCACTTCCTCCAGAATTCGTAGATTACAATAATCAAAGACGTGGGAGAAGAGA
  AAACCAAACAGGTCATCCTGATCACTGTTATGAGAGGAAACCACGGACAATGAATAGTGAAGCTGTTAGT
  GGTCTAAAAATTGAGAAACATTTTAGTGTAAACACTGATTACCCGAGGCCTGTGCAGAGCAATAGTCTTG
  GTGTTCCAAATGGAGAGACAGCTCCGCCTCTGAAAGGAAGGCGCGTAGGACCGATTAAGTCAGCAGGACC
  TGTCACAGCTGTGCCCTACGATGATAAAATATTTTATAATAGTGGGCCCAAAAGAAGGTCTGGGCCAATT
  AAGCCTGAAAAGGTGATAGAATCATCTATTCCTGTGGAGTATGCAAAAGTGTGGAAACCTGGCGATGAAT
  GTTTTGCACTTTATTGGGAAGACAACAAGTTTTACCGAGCAGAAGTTGAAGCCTTGCATTCTTCGGGTAT
  GACAGCGGTCGTTAAGTTCACGGACTACGGCAACTATGAAGAGGTGCTACTGAGCAATATCAAGCCTGTT
  CAGACAGAGGCATGGGTACGTGATCCAAATTCTGTACAAAGGCTACTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001253755
• Insert Size: 2151 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253755.1, NP_001240684.1
• RefSeq Size: 5762 bp
• RefSeq ORF: 2151 bp
• Locus ID: 219249
• UniProt ID: Q91W18
• Cytogenetics: 14 E1
• Gene Summary: Scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. In nucleus, acts as a coactivator: recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci. In cytoplasm, may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks two exons and its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1.