Tspear (NM_001287074) Mouse Untagged Clone

CAT#: MC228627

Tspear (untagged) - Mouse thrombospondin type laminin G domain and EAR repeats (Tspear)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tspear
• Synonyms: C330046G03Rik; ORF65; Tnep1; TSP-EAR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228627 representing NM_001287074
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCGCTGCTGATGTTGTGTGCGGTGCTCTTGCTTCTGGGTACCCCAAGCCGTGGTGCCCGGCCCT
  GGGAGCCCTGCACAGACCTTCGTCCCCTGGACATTCTGGCAGAGGTGGTCCCTTTGAATGGAGCTACAAG
  TGGGATCCGGATGGTGCAGGTGGAGGGTGTGCGGGGCCTCCAGTTCTCAGCCACCGAACCCCGTACCACA
  AGTTTCCCTGCCTCTAGAATCTTCTCCAGCTGCGATTTCTTCCCTGAAGAATTCTCCATCATCGTCACCT
  TGAGAGTTCCCAACCTTCCACCCAAGAAGAATGAATACCTGCTCTCCCTGCTGGCGGAGGAGAGGGACAC
  GCTACTGCTCGGCCTGAGGTACTCACCCACACAGCTGCACTTCCTGTTTCTCAGCGAGGACCTGGCTGGT
  GCCTGGCAGACCCGTGTGTCCTTCTGGAGCCCTGGCCTCATGGACAGCCGATGGCACACTCTGATCCTGG
  CTGTGTCCCAAGGATCCTTTTCTCTCACCACGGACTGTGGCCTCCCGGTGGACATAATGGCTGATGTTTC
  CTTCCCACCCACCCTATCAGTAAGAGGAGCCCGGTTCTTCATTGGCAGCCGGAAAAGAACCAAAGGCCTA
  TTCACAGGTGTGATAAGGCAACTGGTTTTGCTGCCTGGATCAGATGCCACCCCACAGCTGTGTCCCAGCA
  GGAACGCTAGGCTGGCTGAGCTGTCCATCCCCCAGGTGCTGAAGAGGCTCACAGGGAAGCCTGATGATAA
  TGAGGTGCTAAATTATCCCTATGAAGCTGACATGAAAGTAACCCTGGGATCCCGGCCACCGTGTACCAAG
  GCAGAGGGTGCCCAGTTCTGGTTCGATGCTGCCCAAAAGGGGCTATACCTGTGTGCTGGCAGCGAATGGG
  TCTCCGTGTTGGCAGCCAAGACCAAGCTGGACTACGTTGAAGAACACCAGAGTCTGCACACCAACTCAGA
  GACACTGGGCATCGAGGTGTTCAGCATCCCCGGTGTGGGGCTCTTCGCAGCCGCAGCCAACCGGAAGGCT
  AGATCAGCCATCTATAAATGGACAGATGGGAAATTTGTCTCCTATCAGAACATTGCTACGCACCAGGCAC
  AGTCATGGCGACATTTCACCATTGGGAAAAAGATCTTCCTGGCTGTGGCCAACTTTGGGCCAAATGAAAG
  GGGTCAAGAGTTCTCCGTCATCTACAAATGGAGCCCCAGAAAGCTGAAGTTCACCCTCTACCAGAGGATT
  GCCACCCACAGCGCCCGTGACTGGGAGGCCTTTGAGGTGGACGGAGAGCACTTCCTGGTGGTAGCCAACC
  ACCGAGAAGGGGACAACCACAACATCGACAGCATGGTCTACAGGTGGAACCCCAGCAGCCAGCTCTTTGA
  AGCCAATCAGTCCATTGCCACGTCGGGTGCCTACGACTGGGAGTTCTTCACCGTGGGGCCTTACTCCTTC
  CTGGTGGTGGCCAACACCTTCAATGGCACTTCCACCCAGGTGCATTCCCACCTGTACATCTGGCTGGTCG
  GGGCTTTCCAGCTCTTCCAGTCGTTCCTGACATTCGGCGCCGCAGACTGGGAGGTCTTCCACATTGGCGA
  GAGGATCTTCCTTGCAGTGGCAAACAGTCACAGCTACGATGTGCAGATGCAGGCACAAAATGACTCCTAT
  GTCCTCAGCTCTGTTATCTACGAGCTGAACATCACCGCTCAGACCTTTGTCAAGTTCCAGGACATCCCCA
  CCTGCAGTGCCCTGGACTGGGAGTTTTTCTCCGTGGGAGAAGATCACTTCCTGGTGGTTGCGAACTCCTT
  TGACGGAAACACCTTCTCAGTGAACAGTATTATTTACAGGTGGCAAGGCTATGAGGGCTTCGTGGCCGTA
  CACAAACTCCCCACTTTTGGCTGTCGGGACTGGGAGGCTTTTAACACCACTGCTGGCTCTTACCTCATCT
  ACTCCAGCGCCAAGGAGCCCCTCTCCAGGGTCCTGAAGCTGAGGACTGGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001287074
• Insert Size: 2013 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287074.1, NP_001274003.1
• RefSeq Size: 2446 bp
• RefSeq ORF: 2013 bp
• Locus ID: 252974
• UniProt ID: J3S6Y1
• Cytogenetics: 10 C1
• Gene Summary: Plays a critical role in tooth and hair follicle morphogenesis through regulation of the Notch signaling pathway. May play a role in development or function of the auditory system.[UniProtKB/Swiss-Prot Function]