Atg16l1 (NM_001205391) Mouse Untagged Clone

CAT#: MC228505

Atg16l1 (untagged) - Mouse autophagy related 16-like 1 (S. cerevisiae) (Atg16l1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atg16l1
• Synonyms: 1500009K01Rik; Apg16l; Atg16l; WDR30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228505 representing NM_001205391
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCGGGCCTGCGCGCCGCAGACTTTCCCCGCTGGAAGCGTCACATCGCGGAGGAACTGAGGCGCC
  GGGACCGACTGCAGAGGCAGGCGTTCGAGGAGATCATTCTGCAGTATACCAAGTTGCTGGAAAAGTCAGA
  TCTTCATTCAGTATTGACCCAGAAACTACAAGCAGAAAAGCATGACATGCCAAATAGGCATGAAATAAGT
  CCTGGACATGATGGTGCGTGGAATGATAGTCAACTACAAGAAATGGCCCAGTTGAGGATCAAACACCAGG
  AAGAGCTGACCGAACTGCACAAGAAGCGTGGGGAGTTAGCTCAGTTGGTGATTGACCTGAACAACCAAAT
  GCAGCAGAAGGACAAGGAGATACAGATGAATGAAGCAAAGATTTCGGAGTATTTACAGACCATCTCTGAC
  CTGGAGACAAACTGCCTGGACCTGCGCACCAAACTGCAGGACCTCGAGGTAGCCAACCAGACCCTGAAGG
  ATGAGTATGACGCCCTGCAGATTACTTTTACTGCCCTAGAAGAGAAACTGAGGAAAACTACTGAGGAGAA
  CCAGGAACTGGTCACCAGATGGATGGCTGAGAAGGCCCAAGAAGCCAATCGCCTCAATGCAGAGAATGAG
  AAGGACTCCAGGAGGCGTCAAGCACGGCTGCAGAAGGAGCTTGCAGAAGCAGCAAAGGAACCTCTACCTG
  TTGAACAGGATGATGACATTGAAGTCATTGTGGATGAGACCTCAGACCACACAGAAGAGACCTCTCCCGT
  CCGAGCTGTCAGCAGAGCAGCTACTAAGCGACTCTCGCAGCCTGCTGGAGGCCTTCTGGATTCTATCACT
  AATATCTTTGGTCTGTCCGAATCTCCCCTTTTGGGACATCATTCTTCTGATGCTGCCAGGAGACGCTCTG
  TCTCTTCCATCCCAGTCCCCCAGGATATCATGGACACTCATCCTGCTTCTGGTAAAGATGTGAGAGTCCC
  AACTACTGCCTCGTATGTCTTCGATGCGCATGACGGAGAGGTCAACGCAGTGCAGTTCAGTCCAGGCTCC
  CGGTTGCTGGCCACTGGAGGCATGGACCGCAGGGTGAAACTTTGGGAAGCATTCGGAGATAAATGTGAAT
  TCAAGGGCTCCCTGTCTGGCAGTAATGCTGGAATTACAAGCATTGAATTTGATAGTGCTGGAGCTTACCT
  ATTAGCAGCTTCAAATGATTTTGCAAGCCGAATCTGGACTGTGGATGATTATCGATTACGGCACACACTC
  ACAGGCCACAGCGGGAAAGTCCTCTCTGCCAAGTTCCTGCTGGACAATGCACGGATTGTCTCAGGAAGTC
  ACGACCGGACCCTCAAACTCTGGGATCTCCGCAGCAAAGTCTGCATAAAAACAGTGTTTGCAGGATCCAG
  CTGCAATGACATTGTTTGCACTGAACAATGTGTAATGAGTGGACATTTTGACAAGAAAATTCGTTTCTGG
  GATATCCGGTCAGAGAGTGTGGTCCGAGAGATGGAACTGTTAGGGAAGATCACTGCTCTGGACCTAAACC
  CTGAGAGAACTGAGCTCCTGAGCTGCTCCCGTGATGACCTGCTAAAAGTCATCGACCTCCGGACAAATGC
  AGTCAAACAGACATTCAGTGCGCCTGGATTCAAATGCGGCTCTGACTGGACCCGGGTTGTCTTCAGCCCT
  GATGGCAGTTACGTGGCAGCAGGCTCAGCCGAGGGTTCTCTTTATGTCTGGAGTGTGCTGACAGGGAAAG
  TGGAGAAGGTTCTTTCAAAACAGCACAGCTCTTCTATCAATGCGGTGGCGTGGGCCCCCTCGGGCTTACA
  TGTTGTCAGTGTGGACAAAGGAAGCAGAGCTGTGCTGTGGGCACAGCCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205391
• Insert Size: 1872 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205391.1, NP_001192320.1
• RefSeq Size: 3197 bp
• RefSeq ORF: 1872 bp
• Locus ID: 77040
• UniProt ID: Q8C0J2
• Cytogenetics: 1 D
• Gene Summary: Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed:18849966, PubMed:12665549, PubMed:24954904, PubMed:24553140, PubMed:23392225). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (By similarity). Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed:24238340). Instead, promotes with NOD2 an autophagy-dependent antibacterial pathway. Plays a role in regulating morphology and function of Paneth cell.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1, also known as Apg16Lgamma) represents the longest transcript and encodes the longest isoform (1, also known as Apg16Lgamma). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.