Epn2 (NM_001252189) Mouse Untagged Clone

CAT#: MC228377

Epn2 (untagged) - Mouse epsin 2 (Epn2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Epn2
• Synonyms: 9530051D10Rik; AA536924; Ibp2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228377 representing NM_001252189
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAACTTCATCTATCAGACGGCAGATGAAAAACATTGTGAACAATTACTCAGAGGCTGAAATCAAAG
  TCCGGGAGGCCACCTCCAATGACCCGTGGGGCCCATCCAGCTCTCTGATGACTGAGATTGCTGACCTGAC
  CTACAATGTGGTAGCGTTCTCGGAGATCATGAGCATGGTTTGGAAGCGACTTAATGACCATGGCAAGAAC
  TGGCGGCATGTATACAAGGCACTGACACTGCTGGACTACCTTATCAAGACAGGTTCTGAGCGGGTGGCCC
  AGCAGTGCCGTGAGAACATCTTTGCCATACAGACTCTGAAGGACTTTCAGTACATTGACCGTGATGGCAA
  AGACCAGGGTATTAACGTTCGAGAGAAATCAAAGCAACTGGTTGCGCTCCTCAAGGATGAGGAGCGGCTG
  AAGGTTGAGAGAGTTCAGGCTCTCAAAACCAAAGAGCGCATGGCTCAGGTGGCCACTGGCGTGGGCAGCA
  ACCAAATCACCTTTGGCCGTGGCTCCAGCCAGCCCAACCTTTCTACCAGCTACTCAGAGCAGGAGTATGG
  CAAGGCTGGGGGCTCGCCGGCATCCTACCACGGCTCTACTTCCCCACGAGTATCCTCTGAGTTAGAGCAG
  GCCCGGCCACAGACAAGCGGAGAAGAGGAGCTGCAGCTACAACTGGCACTTGCCATGAGCAGAGAGGTTG
  CTGAGCAGGAAGAACGCCTCAGGCGGGGTGATGACCTCAGATTGCAGATGGCTCTGGAAGAAAGCCGGAG
  AGACACAGTAAAAGTTCCAAAAAAGAAAGAGGCGAAAGCTTGTTGCAAGCCAGGCTCCCACTCCCAGCAG
  ACTACCTTGTTGGATTTAATGGATGCCCTCCCCAGCTCAGGCCCTGTTACACAGAAAACTGAGCCCTGGA
  GTGCAGGAGCCTCTGCTAACCAGACCAACCCCTGGGGTGGAACAGTGGCTCCTTCGAACATTACTGACCC
  GTGGCCATCGTTTGGTACCAAGCCAGCTGCCTCTGTGGACCCCTGGGGAGTACCTACCACAGCCAGCACA
  CAGTCTGTCCCTAAGAACTCAGACCCTTGGGCAGCCTCACAGCAGCCTGCCTCCAATGCTGGAAAGACAA
  CAGATGCCTGGGGAGCTGCTAAGCCCAGTTCTGCCTCAGGGTCCTTTGAGCTCTTCAGTAATTTCAACGG
  TACAGTTAAAGATGATTTTTCTGAATTTGACAACCTTCGAACTTCAAAAAAACCAGCTGAGTCAGGGGCC
  TCAGTGCCACCCCAGGACAGCAGAACCACGAGCCCTGACCTCTTTGAGTCTCAATCCTTGACTTCTGCGT
  CAAGCAAGCCTAGCAGTGCCAGGAAAACACCTGAGTCCTTCCTGGGCCCCAATGCAGCCCTGGTGAACCT
  GGACTCATTGGTGACTAAGCCTGCTCCACCAGCTCAGTCCCTTAATCCCTTCCTGGCACCAGGTGCTGCT
  GCTCCAGCCCCTGTCAACCCCTTCCAGGTCAACCAGCCCCAGCCGCTGACACTGAACCAGCTCCGGGGAA
  GCCCTGTCCTGGGAAGCAGTGCATCCTTTGGGTCTGGTCCAGGGGTGGAGACTGTGGCTCCCATGACCTC
  AGTAGCTCCACACTCATCAGTGGGGGCCAGTGGCTCCTCCTTGACACCACTGGGCCCTACAGCAATGAAC
  ATGGTAGGCAGTGTGGGCATTCCCCCATCAGCAGCTCAGTCAACAGGCACAACAAACCCTTTCCTTCTCT
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001252189
• Insert Size: 1752 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252189.1, NP_001239118.1
• RefSeq Size: 4230 bp
• RefSeq ORF: 1752 bp
• Locus ID: 13855
• UniProt ID: Q8CHU3
• Cytogenetics: 11 B2
• Gene Summary: Plays a role in the formation of clathrin-coated invaginations and endocytosis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an in-frame coding exon, and encodes a shorter isoform (3), compared to variant 1.