Impdh1 (NM_001302934) Mouse Untagged Clone

CAT#: MC228339

Impdh1 (untagged) - Mouse inosine 5'-phosphate dehydrogenase 1 (Impdh1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Impdh1
• Synonyms: B930086D20Rik; IMPDH-I
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228339 representing NM_001302934
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAACCGCTCTCACCCCCACCGGAGTCCGGGCTGCTCTCGCCGCTGCTGCAGGGAGGTGGAGCGG
  CTGCCGCTCCAGAGCCCGGAGCCCGGCAACACCCGGGACATGAGACCGCGGCGCAGCGGTACAGCGCCCG
  CCTGCTGCAGGCCGGCTACGAGCCGGAGAGCATGGCGGACTACCTGATCAGCGGCGGCACCGGCTACGTT
  CCCGAGGATGGGCTCACCGCGCAGCAGCTCTTTGCCAACGCGGATGGCCTCACCTACAACGACTTCCTGA
  TCCTCCCAGGATTCATAGACTTCATAGCTGATGAAGTGGACCTGACATCAGCCCTGACCCGGAAGATCAC
  ACTGAAGACACCATTGATCTCGTCTCCCATGGATACAGTGACAGAGGCTGATATGGCCATTGCAATGGCT
  CTCATGGGAGGAATTGGTTTCATTCATCACAACTGTACCCCAGAGTTCCAGGCCAATGAAGTACGGAAGG
  TCAAGAAGTTTGAGCAAGGCTTCATCACAGACCCTGTGGTCCTGAGCCCTTCACATACTGTGGGTGATGT
  TCTGGAGGCCAAGATACAGCATGGCTTCTCTGGTATCCCCATCACCGCGACGGGCACCATGGGGAGCAAG
  CTGGTGGGCATCGTCACCTCCCGAGACATTGACTTCCTTGCTGAGAAGGACCACACCACCCTCCTCAGTG
  AGGTGATGACTCCGAGGGTCGAGCTGGTGGTGGCTCCAGCAGGTGTGACATTGAAAGAAGCAAATGAGAT
  CTTGCAGCGCAGCAAGAAAGGGAAGCTGCCCATAGTCAACGATCAAGATGAGCTGGTAGCCATCATTGCG
  CGCACAGACCTGAAGAAGAACAGAGACTACCCTCTGGCCTCCAAGGACTCCCACAAACAGCTGTTGTGTG
  GGGCAGCTGTGGGCACCCGTGAGGATGACAAATACCGCCTGGACCTGCTCACTCAGGCCGGTGCTGACGT
  CATAGTACTAGATTCATCCCAGGGGAACTCAGTGTATCAGATCGCCATGGTGCACTATATCAAGCAGAAG
  TACCCCCACCTCCAAGTGATTGGGGGAAATGTGGTGACAGCAGCCCAGGCCAAGAACTTGATTGATGCTG
  GTGTGGACGGGCTTCGTGTGGGCATGGGCTGTGGTTCCATCTGCATCACCCAGGAAGTGATGGCCTGTGG
  CCGACCCCAGGGGACTGCTGTCTACAAGGTGGCCGAGTACGCCCGACGTTTTGGGGTGCCGGTAATAGCG
  GATGGTGGCATCCAGACCGTGGGCCATGTGGTCAAAGCCCTGGCACTTGGAGCCTCTACAGTAATGATGG
  GCTCCCTGCTGGCTGCCACCACGGAGGCGCCTGGTGAATACTTCTTCTCAGATGGGGTGAGGCTGAAGAA
  GTACCGGGGCATGGGTTCTCTGGACGCCATGGAGAAGAGCAGCAGCAGCCAGAAAAGATACTTCAGTGAG
  GGGGATAAGGTGAAGATCGCACAAGGTGTCTCCGGTTCCATCCAGGATAAAGGCTCCATTCAGAAGTTTG
  TGCCCTACCTCATAGCAGGGATCCAGCATGGCTGCCAGGATATTGGGGCCCAAAGCCTATCTGTCCTGCG
  ATCCATGATGTACTCAGGAGAGCTCAAGTTTGAGAAGCGGACCATGTCGGCCCAGATTGAGGGTGGCGTG
  CACGGCCTACACTCTTACGAGAAGCGGCTGTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302934
• Insert Size: 1716 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302934.1, NP_001289863.1
• RefSeq Size: 2617 bp
• RefSeq ORF: 1716 bp
• Locus ID: 23917
• UniProt ID: P50096
• Cytogenetics: 6 A3.3
• Gene Summary: Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2 which is shorter by one amino acid compared to isoform 1.