Sass6 (NM_001289568) Mouse Untagged Clone

CAT#: MC228308

Sass6 (untagged) - Mouse spindle assembly 6 homolog (C. elegans) (Sass6), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sass6
• Synonyms: 2810453L12Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228308 representing NM_001289568
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGAACACGCGAAAGAGACTCCAAGGTTCCTGCTGCAGCTTCTTTCTTCAGCCACTCTTTTGGAGA
  ACTCACCGGTCCTTTTAAACGTAGTGGAGACAAATCCTTTTAAGCATCTTATTCACCTGTCACTAAAGCT
  TTTACCTGGAAATGATGTAGAAATAAAGAAATTTCTAGCAGGATGTTTGAAATGTAGCAAGGAAGAAAAA
  TTATCACTGACTAGATCACTAGATGATGTTACCAGGCAACTGCACATTACACAAGAGACGTTGTCGGAAA
  AAATGCAAGAATTAGATAAGCTGCGGAGTGAATGGGCCTCGCACACAGCATCGCTGACGAATAAGCACTC
  TCAGGAGTTAACAGCTGAGAAGGAGAAGGCCTTGCAGACCCAAGTTCAGTGCCAACAGCAGCACGAGCAA
  CAGAAAAAGGAACTGGAAACCCTCCATCAACGGAATATCCACCAGCTACAAAGCAGATTGTCCGAGTTAG
  AGGCAGCTAATAAAGAGCTCACCGAGAGGAAGTATAAAGGAGACTCGACTGTCCGAGAGCTAAAGGCGAA
  GCTGGCTGGCGTGGAAGAGGAGCTGCAGCGGGCCAAGCAAGAGGTCCTTTCTCTGAGAAGAGAGAATTGT
  ACTCTGGATACTGAATGCCACGAGAAAGAAAAGCACATCAACCAGCTACAAACAAAAGTGGCCGTTTTAG
  AACAGGAGATCAAAGATAAAGACCAGCTTGTTCTGAGAACAAAAGAAGCATTCGATACAATCCAAGAGCA
  AAAGGTGGCTTTAGAAGAAAATGGTGAGAAAAATCAGATACAACTGGGAAAACTTGAAGCTACAATAAAA
  TCATTATCAGCAGAACTTCTAAAGGCAAATGAGATCATCAAGAAGTTACAAGGAGATCTTAAAACTCTGA
  TGGGTAAATTGAAACTGAAGAATACAGTTACTATTCAGCAAGAAAAACTATTGGCTGAGAAAGAAGAAAT
  GCTACAAAAGGAGCGAAAGGAATCACAGGACGCTGGGCAGTTTCTTCGTGCCAAAGAGCAAGAGGTATGC
  AGATTACAGGAACAATTAGAAACTACAGTTCAGAAACTCGAAGAAAGTAAACAACTTTTGAAAAATAATG
  AAAAATTAATCACGTGGCTAAACAAAGAGCTAAATGAAAATCAGCTGGTAAGAAAACAGGACACATTGGG
  AACCTCTGCCACCCCACATTCTACTAGCAACAGCACCATCAGAAGTGGGCTCTCTCCCAATCTGAATGTG
  GTTGATAGACTAAATTACCCAAGCTGTGGAATTGGCTATCCTGTCTCCTCTGCATTGACATTCCAGAATG
  CTTTTCCTCATGTAGTAGCTGCCAAAAACACCAGCCACCCTATCTCTGGACCAAAGGTTCACTTTAACCT
  GCAGCTTACAAAACCAAGCGCTTCGATAGATGGGCAGCCGGGAGCCGCTGTTAACAGGCCTTGTTCAAAT
  GATAAGGAAAATGGTGAAACTTTAGGATTGGAATCCAAATACTTGAAGAGAAGAGAAGCTAGCATTCCTT
  TACGCGGACTTAGCCAGAATCTGTTGAGTGACTCAGACCATCAGAAAGACGGCATGCTGGGAGCGTTCCA
  GCTATCTTCCAAACCCACCGTTCTCCCCTCCTCGTCTTCAGCCTACTTCCCTGGGCAGTTACCAAGTAGT
  TAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289568
• Insert Size: 1683 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289568.1, NP_001276497.1
• RefSeq Size: 4392 bp
• RefSeq ORF: 1683 bp
• Locus ID: 72776
• UniProt ID: Q80UK7
• Cytogenetics: 3 G1
• Gene Summary: Central scaffolding component of the centrioles ensuring their 9-fold symmetry. Required for centrosome biogenesis and duplication: required both for mother-centriole-dependent centriole duplication and deuterosome-dependent centriole amplification in multiciliated cells (PubMed:24240477). Required for the recruitment of STIL to the procentriole and for STIL-mediated centriole amplification (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) contains an alternate exon, that causes translation initiation at a downstream start codon, compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.