Eya2 (NM_001271963) Mouse Untagged Clone

CAT#: MC228201

Eya2 (untagged) - Mouse eyes absent 2 homolog (Drosophila) (Eya2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eya2
• Synonyms: Eab1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228201 representing NM_001271963
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTAGAAGTGGTGACCTCACCCAGCCTCGCAACAAGCAGTGACTGGAGCGAGCACGGTGCTGCCGTGG
  GGACGCTGAGTGACAGGGAAGGCATCGCCAAATCAGCGGCTCTGAGTGTGCCTCAGCTCTTTGTGAAGTC
  TCATCCACGTGTCCCTCCTGGTCAGTCCTCCACAGCCATGGCGGCCTATGGCCAGACACAGTACAGCACA
  GGCATTCAGCAGGCACCACCCTATACAGCGTACCCAACTCCGGCGCAAGCCTATGGAATCCCCCCTTACA
  GCATCAAGACAGAAGACAGTTTGAATCACTCCCCCAGCCAGAGCGGGTTCCTGAGCTATGGACCGAGCTT
  CAGCACCGCGCCTGCTGGACAGAGCCCCTACACCTACCCCGTGCACAGCACCGCTGGGCTCTATCAAGGC
  GCCAACGGACTGACCAACACCGCTGGATTTGGGAGCGTGCACCAGGATTATCCGTCCTACCCCAGCTTTT
  CACAGAACCAGTACCCCCAGTATTTCAGCCCATCATACAACCCGCCCTACGTCCCTGCCAGCAGCCTCTG
  CTCCTCGCCCCTCTCCACGTCCACCTACGTCCTCCAGGAGGCTCCTCACAATGTCCCCAGCCAGAGTTCT
  GAGTCCCTGGCCGGAGACTACAACACACACAACGGACCCTCCACACCAGCAAAGGAGGGTGACACAGAGA
  GGCCACATCGAGCCTCGGATGGGAAGCTACGGGGCCGGTCAAAGAGAAATAGTGACCCTTCCCCAGCAGG
  AGACAATGAAATCGAGCGCGTGTTCGTCTGGGACCTGGACGAGACAATCATTATCTTCCACTCCCTGCTC
  ACAGGGACGTTTGCATCCAGATACGGGAAGGACACCACGACGTCTGTGCGCATTGGCCTGATGATGGAGG
  AGATGATCTTCAACCTTGCTGACACACACCTGTTCTTCAATGACCTGGAGGACTGTGACCAAATCCACGT
  GGATGATGTCTCATCCGATGACAATGGTCAGGATTTAAGCACATACAACTTCTCCACTGATGGCTTCCAC
  AGCACGGCGCCAGGAGCCAGCTTGTGCCTGGGTACAGGTGTTCATGGCGGTGTGGACTGGATGAGGAAAC
  TGGCCTTCCGCTACCGTCGTGTGAAGGAGATGTACAACACCTACCGCAACAACGTGGGTGGCTTGATAGG
  TGCTCCCAAAAGAGAGACCTGGCTGCAGCTGCGCGCCGAGCTGGAGGCCCTGACTGACCTCTGGCTCACC
  CACTCCCTGAAAGCCCTCAATCTCATCAACTCTCGACCCAACTGTGTCAATGTGTTGGTCACCACCACGC
  AACTGATCCCTGCATTGGCCAAGGTCCTGCTGTACGGTCTGGGCTCCGTGTTCCCCATCGAGAACATCTA
  CAGTGCGACCAAGACAGGCAAGGAGAGCTGCTTCGAAAGAATCATGCAGAGGTTTGGCCGCAAAGCTGTC
  TACATTGTGATAGGCGACGGGGTAGAGGAAGAGCAAGGAGCCAAAAAGCACAACATGCCTTTCTGGAGGA
  TATCCTGTCATGCTGACCTGGAGGCTCTAAGGCATGCCCTGGAACTGGAGTATCTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001271963
• Insert Size: 1599 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271963.1, NP_001258892.1
• RefSeq Size: 2459 bp
• RefSeq ORF: 1599 bp
• Locus ID: 14049
• UniProt ID: O08575
• Cytogenetics: 2 85.83 cM
• Gene Summary: This gene encodes a member of the eyes absent protein family. The encoded protein is a tyrosine phosphatase which acts as a transcriptional activator during development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.