Pus10 (NM_001033654) Mouse Untagged Clone

CAT#: MC228176

Pus10 (untagged) - Mouse pseudouridylate synthase 10 (Pus10), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pus10
• Synonyms: 2810013G11Rik; 4933435A13Rik; AU014648; C77560; Ccdc139
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228176 representing NM_001033654
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCCACTGACTGAGGAGAACAAGCACGTGGCACAGCTGTTGTTCAGTTCTGGCACCTGTCCAAGAT
  GCATCTTAAGATTCTGTGGTGTGGATCTTCCCGCACCTTACAAACACCCATCCAAGGAGTTGCTCAATGA
  GCTACAAAAATTTCTAGAACCGGAAAAACCTGAATTAATTTTAGAAGCTCCAAACCCACCATTGAAGAAA
  ATTCGTCTACATGAAGATGGGATTGATAATTTGAGTGAAGATGGAAAGGAGGGAGTCTCTGTTACTGAAG
  ATGAAAGCATGGCTGAGAAGCCTTCAAAGCTGAGAGTGTGCAATGTTTGCTTAGGAATTCTTCAGGAATT
  CTGTGAGAAGGGGTTTATTACGAAGGTGTGCCAAAAGGTTGAAGCCTCTGGGTTTGAATTCACCAGCGTG
  GTTTTATCTGTCTCCTTTCCACCGCAGCTGTCTGTAAGAGAGCATGCTGCATGGTTGCTGGTAAAACAGG
  AAATGGGAAAGCAGAGTCTGTCCCTGGGAAGAAATGATGTAGTCCAGCTGAAAGAAGCCTACAAGTGGAT
  AACTCACCCCCTGTTTTCAGAGGAGCTGGGCGTTCCCACTGATGGAAAGAGCTTGTTCGAAGTGAGCGTG
  GTCTTTGCTCATCCAGAAACAGCTGAAGATTGCCATTTCCTAGGTGAAGTTTGCCGAGATTGTTTCAAAC
  CAGCTAAAAATAAACAGTCGGTATTTACCAGAATGGCAGTTTTGAAAGCTTTGAGTAAGATAAAAGAAGA
  AGACTTCCTCGGGCAATTTCCTTGTCCTCCAAACTCACCGAAGACTGTATGCACTGTCCTTGAAGTTGAG
  TGCACTCATGGTGCTGTTTTTGTCGCTGGCAGATATAATAAATACTCCAGGAATCTACCACAAACTCCTT
  GGATAATTGATGGAGAAAGGAAAATGGAATCTTCGGTGGAAGAATTAATTTCAGATCATCTTCTGGCAGT
  ATTCAGAGCAGAGAGTTTTAATTTCTCATCCTCGGGACGAGAAGACGTAGACGTGAGAACATTAGGAAAT
  GGAAGGCCCTTTGCAGTTGAACTTCTGAATCCTCATCGAGTGCATTTCACCTCACAAGAAATGAAGGAGC
  TCCAGCAGACAATTAATAAATCATCTGACAAAATCCAAGTCCGTGACTTGCAGCTTGTCACCAGGGAGGC
  AATAGGGCATATGAAGGAAGGTGAGGAAGAAAAGACTAAGACCTACAGTGCCTTGATATGGACGAATAGA
  GCTATCCAGAAGAAGGACATTGGGTTCCTGGACGACTTAAAGGACTTAAAGATTGACCAGAAAACACCTC
  TCCGAGTCCTTCACCGAAGGCCTTTGGCTGTGAGAACTCGGGCCATTCACTCTATGAAGACACACTACCT
  GGATGAGCATCATTTTCGTCTGCATTTGAAGACCCAAGCTGGAACCTACATTAAAGAATTTGTACATGGA
  GACTTTGGGAGAACCAAACCAAACCTTGGCTCTTTGATGAACGTGACTGCAGACATTCTTGAGCTGGATG
  TGGAGTCTGTAGATGTTGACTGGCCGCCTGCTCTGGATGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001033654
• Insert Size: 1584 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033654.2, NP_001028826.1
• RefSeq Size: 3177 bp
• RefSeq ORF: 1584 bp
• Locus ID: 74467
• UniProt ID: Q9D3U0
• Cytogenetics: 11
• Gene Summary: Pseudouridylate synthases catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. PUS10 catalyzes the formation of the universal psi55 in the GC loop of transfer RNAs (Probable). Modulator of TRAIL-induced cell death via activation of procaspase 8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death (By similarity).[UniProtKB/Swiss-Prot Function]