Rufy3 (NM_001289775) Mouse Untagged Clone

CAT#: MC228137

Rufy3 (untagged) - Mouse RUN and FYVE domain containing 3 (Rufy3), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rufy3
• Synonyms: 2810428M05Rik; 6330416M07Rik; AW455998; AW538594; D5Bwg0860e; Ripx; Rpipx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228137 representing NM_001289775
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGTCGCCGGCGCCCGGCGCGGCTGCTGAAAGTTGCGGCGAAGAGCAGGAGAGGGGCGGGGAGC
  GGCGCCCCTCCGAGCCGCTGGAACCGCGCGGTGCTAGCGCCCGCGGCGCGGACCGGGAGGACGAGGCGGG
  GCCCTCGGAGCCGGACTCGCCGGTGGCCGCCCCCTTCTTCCTCCTCTACCCGGGCGATGGAGGCGCGGGC
  TTCACAGCGCGCCCGCCACCGCAGCGCGCCTGGAGGACCCCGCCGTCGCCCGGCTCCCCGCTGCCCTTCC
  TGCTGCTGAGCTACCCGAGCGGCGGCAGCGGCGGCGGAGGCAAGCACCATCCCAATTATCTCATGGCTAA
  CGAACGCATGAACCTGATGAACATGGCAAAGCTGAGCATCAAGGGCTTGATTGAATCGGCTCTGAATCTG
  GGGCGGACCCTGGACTCTGACTACGCACCTCTCCAGCAGTTTTTCGTGGTGATGGAACACTGCCTGAAAC
  ACGGCTTGAAAGCCAAGAAAACTTTTCTTGGACAAAATAAATCCTTCTGGGGGCCTCTAGAGCTGGTGGA
  GAAGCTTGTTCCAGAAGCTGCAGAAATAACAGCGAGTGTAAAAGACCTCCCAGGACTCAAGACACCAGTT
  GGCAGAGGAAGAGCCTGGCTTCGGTTGGCATTGATGCAAAAGAAGCTTTCTGAGTATATGAAAGCCTTGA
  TCAATAAGAAGGAACTTCTCAGTGAGTTCTATGAAGTCAATGCCCTCATGATGGAAGAAGAAGGAGCTAT
  TATCGCTGGTCTCCTGGTGGGTCTGAATGTCATCGATGCCAATTTCTGCATGAAAGGAGAAGACCTGGAC
  TCTCAGGTTGGAGTTATAGATTTTTCGATGTATCTCAAAGATGGAAACAGTAGTAAAGGTAGTGAAGGGG
  ATGGACAGATTACTGCGATCCTAGACCAGAAGAACTATGTAGAAGAACTCAACAGACATCTGAATGCAAC
  TGTAAACAACCTTCAGACAAAAGTAGATCTGTTAGAAAAATCCAACACTAAATTGACAGAAGAACTTGCC
  GTTGCCAACAACAGAATTATTACCTTACAAGAAGAAATGGAACGAGTTAAAGAAGAAAGCTCCTATCTAC
  TGGAATCCAATCGGAAGGGTCCTAAACAAGACAGAACTGCAGAAGGGCAAGCGCTGAGCGAAGCCAGAAA
  GCATCTAAAGGAGGAGACACAGTTAAGATTGGATGTCGAGAAGGAGCTGGAGCTGCAGATCAGCATGAGG
  CAGGAGATGGAACTGGCTATGAAGATGCTGGAGAAGGATGTCTGTGAGAAGCAGGATGCCCTGGTGTCTC
  TGCGGCAGCAGCTGGACGATCTCCGGGCTCTTAAGCATGAGCTCGCCTTTAAACTGCAGAGTTCAGACCT
  AGGAGTGAAACAGAAAAGTGAATTAAACAGTCGCTTGGAAGAAAAGACCAATCAGATGGCTGCCACCATT
  AAACAGCTGGAGCAAAGTGAAAAAGATTTGGTGAAACAGGCAAAGACCTTAAATAGTGCAGCAAATAAAC
  TGATCCCAAAGCACCATTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289775
• Insert Size: 1560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289775.1, NP_001276704.1
• RefSeq Size: 3729 bp
• RefSeq ORF: 1560 bp
• Locus ID: 52822
• UniProt ID: Q9D394
• Cytogenetics: 5 43.77 cM
• Gene Summary: Plays a role in the generation of neuronal polarity formation and axon growth (PubMed:24720729). Implicated in the formation of a single axon by developing neurons (PubMed:24720729). May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes (By similarity). Plays a role in the formation of F-actin-enriched protrusive structures at the cell periphery (By similarity). Plays a role in cytoskeletal organization by regulating the subcellular localization of FSCN1 and DBN1 at axonal growth cones (PubMed:24720729). Promotes gastric cancer cell migration and invasion in a PAK1-dependent manner (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks several exons in the 3' coding region and contains an alternate 3' terminal exon, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct C-terminus, compared to isoform 1.