Traf2 (NM_001290413) Mouse Untagged Clone

CAT#: MC228093

Traf2 (untagged) - Mouse TNF receptor-associated factor 2 (Traf2), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Traf2
• Synonyms: AI325259
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228093 representing NM_001290413
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCAGCCAGTGTGACTTCCCCTGGCTCCCTAGAACTGCTACAGCCTGGCTTCTCCAAGACCCTCC
  TGGGGACCAGGTTAGAAGCCAAGTACCTCTGTTCAGCCTGCAAAAACATCCTGCGGAGGCCTTTCCAGGC
  CCAGTGTGGGCACCGCTACTGCTCCTTCTGCCTGACCAGCATCCTCAGGTGTGCATCCATTCTCTCCAGC
  TCTGGGCCCCAGAACTGTGCTGCCTGTGTCTATGAAGGCCTGTATGAAGAAGGCATTTCTATTTTAGAGA
  GTAGTTCGGCCTTTCCAGATAACGCTGCCCGCAGAGAGGTGGAGAGCCTGCCAGCTGTCTGTCCCAATGA
  TGGATGCACTTGGAAGGGGACCTTGAAAGAATACGAGAGCTGCCACGAAGGACTTTGCCCATTCCTGCTG
  ACGGAGTGTCCTGCATGTAAAGGCCTGGTCCGCCTCAGCGAGAAGGAGCACCACACTGAGCAGGAATGCC
  CCAAAAGGAGCCTGAGCTGCCAGCACTGCAGAGCACCCTGTAGCCACGTGGACCTGGAGGTACACTATGA
  GGTCTGCCCCAAGTTTCCCTTAACCTGTGATGGCTGTGGCAAGAAGAAGATCCCTCGGGAGACGTTTCAG
  GACCATGTTAGAGCATGCAGCAAATGCCGGGTTCTCTGCAGATTCCACACCGTTGGCTGTTCAGAGATGG
  TGGAGACTGAGAACCTGCAGGATCATGAGCTGCAGCGGCTACGGGAACACCTAGCCCTACTGCTGAGCTC
  ATTCTTGGAGGCCCAAGCCTCTCCAGGAACCTTGAACCAGGTGGGGCCAGAGCTACTCCAGCGGTGCCAG
  ATTTTGGAGCAGAAGATAGCAACCTTTGAGAACATTGTCTGCGTCTTGAACCGTGAAGTAGAGAGGGTAG
  CAGTGACTGCAGAGGCTTGTAGCCGGCAGCACCGGCTAGACCAGGACAAGATTGAGGCCCTGAGTAACAA
  GGTGCAACAGCTGGAGAGGAGCATCGGCCTCAAGGACCTGGCCATGGCTGACCTGGAGCAGAAGGTCTCC
  GAGTTGGAAGTATCCACCTATGATGGGGTCTTCATCTGGAAGATCTCTGACTTCACCAGAAAGCGTCAGG
  AAGCCGTAGCTGGCCGGACACCAGCTATCTTCTCCCCAGCCTTCTACACAAGCAGATATGGCTACAAGAT
  GTGTCTACGAGTCTACTTGAATGGCGACGGCACTGGGCGGGGAACTCATCTGTCTCTCTTCTTCGTGGTG
  ATGAAAGGCCCCAATGATGCTCTGTTGCAGTGGCCTTTTAATCAGAAGGTAACATTGATGTTGCTGGACC
  ATAACAACCGGGAGCATGTGATCGACGCATTCAGGCCCGATGTAACCTCGTCCTCCTTCCAGAGGCCTGT
  CAGTGACATGAACATCGCCAGTGGCTGCCCCCTCTTCTGCCCTGTGTCCAAGATGGAGGCCAAGAATTCC
  TATGTGCGGGATGATGCGATCTTCATCAAAGCTATTGTGGACCTAACAGGACTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290413
• Insert Size: 1527 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290413.1, NP_001277342.1
• RefSeq Size: 3065 bp
• RefSeq ORF: 1527 bp
• Locus ID: 22030
• UniProt ID: P39429
• Cytogenetics: 2 A3
• Gene Summary: Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Isoform 2 does not seem to mediate activation of NF-kappa-B but inhibits isoform 1 activity. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).