Rbck1 (NM_019705) Mouse Untagged Clone

CAT#: MC228090

Rbck1 (untagged) - Mouse RanBP-type and C3HC4-type zinc finger containing 1 (Rbck1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rbck1
• Synonyms: AL033326; HOIL-1; HOIL-1L; Ubce7ip3; UIP28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228090 representing NM_019705
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAGAAGACCAAGAAAGCAGAGGAGATGGCCCTGAGCCTTGCCCGGGCAGTGGCTGGCGGAGATG
  AACAGGCTGCTATCAAGTATGCCACCTGGCTGGCAGAGCAGAGGGTGCCCCTCAGGGTGCAAGTAAAACC
  CGAGGTCTCCCCAACACAGGACATCAGGCTCTGTGTGAGTGTGGAGGATGCGTACATGCACACCGTCACC
  ATTTGGCTCACAGTACGCCCGGATATGACAGTGGCCTCCCTTAAGGACATGGTATTCCTGGACTATGGCT
  TCCCACCTAGCCTGCAGCAGTGGGTGGTTGGACAGAGGCTGGCACGAGACCAGGAGACCTTGCATTCACA
  CGGCATTCGGCGGAATGGAGACGGTGCCTATCTCTACCTGCTGTCAGCCCGCAACACGTCACTCAACCCA
  CAAGAGCTGCAGCGGCAGCGGCAACTGCGAATGTTGGAAGATTTGGGCTTCAAGGACCTCACCCTTCAGT
  CACGGGGGCCCTTGGAACCTGTCCTTCCGAAGCCCAGGACCAACCAGGAGCCGGGACAGCCAGATGCAGC
  ACCGGAGTCACCACCGGTGGGCTGGCAGTGCCCTGGCTGCACTTTCATCAACAAACCCACACGGCCTGGG
  TGTGAGATGTGCTGTCGTGCAAGGCCTGAGACCTACCAGATACCTGCTTCATACCAGCCTGACGAGGAAG
  AGCGAGCACGCCTGGCCGGCGAGGAGGAGGCGCTGCGCCAGTACCAGCAGCGGAAACAGCAGCAGCAGGA
  GGGGAACTACCTGCAGCACGTGCAGCTGGAGCAGAGGAGCCTGGTGCTGAACACCGAACCTACTGAGTGC
  CCTGTGTGCTACTCAGTGCTGGCACCCGGCGAGGCCGTGGTGCTGCGTGAGTGTCTGCACACCTTCTGCA
  GGGAGTGCCTGCAGGGCACCATCCGAAACAGCCAGGAGGCGGAGGTGGCTTGCCCCTTCATTGACAGCAC
  CTACTCATGCCCCGGCAAGCTGCTGGAGAGAGAGATCCGGGCGCTCCTGTCCCCTGAGGACTACCAGCGT
  TTCCTGGATCTGGGTGTGTCCATCGCAGAGAACCGAAGCACCTTGAGCTACCACTGCAAGACCCCCGACT
  GCAGGGGTTGGTGCTTCTTTGAGGATGATGTCAACGAGTTCACCTGTCCTGTGTGCACCCGTGTCAACTG
  CCTGCTCTGCAAGGCCATCCATGAGCACATGAATTGCAGGGAGTACCAAGACGACCTGGCCCTGCGGGCT
  CAGAATGATGTGGCTGCCCGGCAGACGACAGAGATGCTAAAGGTAATGCTGCAGCAGGGCGAGGCCATGC
  ACTGCCCACAGTGCCGGATTGTGGTGCAGAAGAAGGACGGCTGTGACTGGATCCGCTGTACAGTCTGCCA
  CACTGAGATCTGCTGGGTCACCAAGGGCCCACGCTGGGGCCCAGGGGGCCCAGGGGACACCAGTGGTGGC
  TGCCGCTGCCGGGTCAATGGGATTCCATGCCACCCAAGCTGTCAAAACTGCCACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_019705
• Insert Size: 1527 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019705.3, NP_062679.2
• RefSeq Size: 2251 bp
• RefSeq ORF: 1527 bp
• Locus ID: 24105
• UniProt ID: Q9WUB0
• Cytogenetics: 2 G3
• Gene Summary: E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a).