Mavs (NM_001206385) Mouse Untagged Clone

CAT#: MC228071

Mavs (untagged) - Mouse mitochondrial antiviral signaling protein (Mavs), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mavs
• Synonyms: cardif; D430028G21Rik; IPS-1; Visa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC228071 representing NM_001206385
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACATTTGCTGAGGACAAGACCTATAAGTATATCCGAGACAACCACAGCAAGTTTTGCTGTGTTGACG
  TTCTGGAGATCCTGCCTTACCTGTCCTGCCTCACAGCTAGTGACCAGGATCGACTGCGGGCTTCCTACAG
  GCAGATCGGGAACCGGGACACACTCTGGGGACTCTTCAATAATCTCCAGCGCCGGCCTGGCTGGGTGGAG
  GTCTTCATCCGGGCACTGCAGATCTGTGAGCTGCCTGGGCTGGCTGATCAAGTGACTCGAGTTTATCAGA
  GCTACCTGCCTCCGGGGACCTCACTCCGCTCCCTAGAGCCACTGCAGTTACCAGACTTTCCTGCTGCGGT
  TTCTGGACCCTCTGCATTTGCGCCAGGTCACAACATCCCTGACCATGGCTTACGAGAGACACCAAGTTGC
  CCCAAGCCTGTCCAGGACACCCAGCCACCAGAGTCCCCAGTAGAGAATTCAGAGCAACTCCTCCAGACCA
  ACTCCGGGGCCGTCGCGAGGATGTCTGGTGGCTCTTTGATACCCTCTCCTAACCAGCAGGCTCTCAGCCC
  TCAGCCCTCCAGAGAGCATCAAGAGCAAGAACCAGAACTGGGTGGCGCCCACGCAGCAAATGTTGCCTCT
  GTTCCCATAGCAACCTATGGACCTGTGTCTCCAACCGTTTCCTTCCAGCCCCTTCCACGTACTGCCCTGA
  GGACAAACCTCTTGTCTGGGGTCACAGTATCAGCCCTATCTGCTGATACCTCTTTGTCCTCCTCGTCCAC
  TGGATCAGCTTTTGCAAAGGGAGCTGGTGACCAGGCCAAAGCTGCCACCTGTTTCAGTACTACACTCACC
  AATTCTGTGACTACCAGCTCAGTGCCTTCTCCCAGATTGGTCCCAGTAAAAACCATGTCTTCCAAGTTGC
  CCCTCAGTTCAAAGTCCACTGCTGCGATGACGTCTACTGTGCTCACCAATACAGCGCCATCAAAATTACC
  CAGCAACTCAGTGTATGCGGGCACAGTGCCATCCAGAGTGCCTGCTAGTGTGGCCAAAGCACCTGCCAAC
  ACAATACCACCTGAGAGGAACAGCAAGCAAGCCAAGGAGACCCCGGAGGGTCCAGCAACCAAAGTCACCA
  CTGGAGGCAACCAGACTGGACCAAATAGCAGTATCAGGAGCTTGCACTCTGGACCAGAGATGAGCAAGCC
  AGGTGTGCTGGTATCCCAGTTGGACGAGCCATTCTCAGCCTGCTCTGTGGACCTTGCCATTAGCCCTAGC
  AGCTCCTTGGTCTCAGAACCCAACCATGGTCCAGAGGAGAATGAGTATTCGTCCTTTAGAATCCAGGTAG
  ACGAAAGCCCCAGTGCTGATCTATTAGGAAGCCCTGAGCCACTAGCCACCCAGCAGCCCCAAGAAGAGGA
  AGAACATTGTGCCAGTTCAATGCCCTGGGCTAAGTGGCTTGGGGCCACCAGTGCACTCTTGGCTGTATTC
  CTGGCAGTGATGCTGTACCGTAGTAGGCGCCTGGCCCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001206385
• Insert Size: 1512 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001206385.1, NP_001193314.1
• RefSeq Size: 2871 bp
• RefSeq ORF: 1512 bp
• Locus ID: 228607
• UniProt ID: Q8VCF0
• Cytogenetics: 2 F1
• Gene Summary: Required for innate immune defense against viruses (PubMed:24037184). Acts downstream of DHX33, DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5) (PubMed:24037184). Peroxisomal and mitochondrial MAVS act sequentially to create an antiviral cellular state (By similarity). Upon viral infection, peroxisomal MAVS induces the rapid interferon-independent expression of defense factors that provide short-term protection, whereas mitochondrial MAVS activates an interferon-dependent signaling pathway with delayed kinetics, which amplifies and stabilizes the antiviral response (By similarity). May activate the same pathways following detection of extracellular dsRNA by TLR3 (By similarity). May protect cells from apoptosis (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.