Mdm4 (NM_001302802) Mouse Untagged Clone

CAT#: MC227981

Mdm4 (untagged) - Mouse transformed mouse 3T3 cell double minute 4 (Mdm4), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mdm4
• Synonyms: 4933417N07Rik; AA414968; AL023055; AU018793; AU021806; C85810; Mdmx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227981 representing NM_001302802
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACATCACATTCCACCTCGGCCCAGTGTTCAGCATCTGACAGTGCTTGCAGAATTTCTTCGGAACAAA
  TTAGTCAGCAGGTGCGGCCAAAACTGCAGCTTTTGAAGATTTTGCATGCAGCAGGTGCGCAGGGGGAAGT
  ATTCACCATGAAAGAGGTAATGCACTATCTAGGCCAGTATATAATGGTGAAGCAGCTCTATGATCAACAG
  GAGCAACATATGGTATACTGTGGTGGAGATCTTTTGGGAGATCTACTTGGATGTCAGAGCTTTTCTGTGA
  AAGATCCAAGCCCTCTCTATGACATGCTAAGAAAGAATCTTGTTACATCAGCTTCTATTAACACAGATGC
  TGCTCAGACTCTCGCTCTCGCACAGGATCACACTATGGATTTTCCAAGTCAAGACCGACTGAAGCACGGT
  GCAACAGAATACTCCAATCCCAGAAAAAGAACTGAAGAAGAGGATACTCACACACTGCCTACCTCACGAC
  ATAAATGCAGAGACTCCAGAGCAGATGAAGACTTGATAGAACATTTATCTCAAGATGAGACATCTAGGCT
  TGACCTTGATTTTGAGGAGTGGGACGTTGCTGGCCTGCCTTGGTGGTTTCTAGGGAATTTGAGAAACAAC
  TGTATTCCTAAAAGTAATGGCTCAACTGATTTACAGACAAATCAGGATATAGGTACTGCCATTGTTTCAG
  ACACTACGGATGATTTGTGGTTTTTAAATGAGACCGTGTCAGAGCAATTAGGTGTTGGAATAAAAGTTGA
  AGCTGCTAATTCTGAGCAAACAAGTGAAGTAGGGAAAACAAGTAACAAGAAGACGGTGGAGGTGGGAAAG
  GATGATGATCTTGAGGACTCCAGGTCCTTGAGCGATGATACTGACGTGGAACTTACCTCTGAGGATGAGT
  GGCAGTGTACGGAATGCAAGAAGTTTAATTCTCCAAGCAAGAGGTACTGTTTTCGTTGCTGGGCCTTGAG
  AAAGGATTGGTATTCGGATTGTTCTAAATTAACTCATTCCCTATCTACATCTAATATTACTGCCATACCT
  GAAAAGAAGGACAATGAAGGAATTGATGTTCCCGATTGTAGGAGAACCATTTCAGCTCCTGTTGTTAGGC
  CTAAAGATGGATATTTAAAGGAGGAAAAGCCCAGGTTTGACCCTTGCAACTCAGTGGGATTTTTGGATTT
  GGCTCATAGTTCTGAAAGCCAGGAGATCATCTCAAGCGCGAGAGAACAAACAGATATTTTTTCTGAGCAG
  AAAGCTGAAACAGAAAGTATGGAAGATTTCCAGAATGTCTTGAAGCCGTGTAGCTTATGTGAAAAAAGGC
  CTCGGGATGGGAACATTATTCATGGGAAGACGAGCCATCTGACGACATGTTTCCACTGTGCCAGGAGACT
  GAAGAAGTCTGGGGCTTCGTGTCCTGCTTGTAAGAAAGAGATTCAGTTGGTTATTAAAGTTTTTATAGCA
  TAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001302802
• Insert Size: 1473 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302802.1, NP_001289731.1
• RefSeq Size: 7107 bp
• RefSeq ORF: 1473 bp
• Locus ID: 17248
• UniProt ID: O35618
• Cytogenetics: 1 57.75 cM
• Gene Summary: This gene encodes a protein that has been shown to negatively regulate the activity of the tumor suppressor protein p53. Homozygous knockout mice exhibit embryonic lethality as a result of p53-dependent apoptosis and cell cycle arrest. Amplification of this gene or overexpression of the encoded protein has been linked to a range of human cancers. A pseudogene has been identified on the X chromosome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1).