Tmem39a (NM_001205286) Mouse Untagged Clone

CAT#: MC227946

Tmem39a (untagged) - Mouse transmembrane protein 39a (Tmem39a), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tmem39a
• Synonyms: 2610033C09Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227946 representing NM_001205286
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCAGTAGAAGGCGGGGTCCCAGTCGGCAGCAGCTAAGCCGGTCAGCTTTACCTTCCATACAGACGT
  TGGTCGGCGGCGGCTGTGGCAATGGGACGGGCTTGAGGAACAGGAATGGTAATGCTATTGGCCTTCCAGT
  CCCACCCACCACAGCCTTAATTACCCCAGGTCCTGTTCGTCATTGCCAAATCCCTGACTTACCAGTAGAT
  GGGAGCCTGTTCTTTGAATTTCTTTTCTTCATCTACCTGCTGATTGTTCTGTTCATTCAGTATATCAACA
  TCTATAAGACGGTGTGGTGGTATCCGTACAATCATCCTGCTTCTTGTACTTCACTGAATTTTCACCTCAT
  TGATTATTACCTGGCAGCATTCATCACAGTGATGCTTGCGAGGAGGCTTGTATGGGCCCTCATTTCCGAG
  GCTACTAAAGCAGGTGCTGCGTCTACGGTTCACTACACAGCTCTGATCCTGGCCCGCTTGGTGCTCCTTA
  CTCTGTGTGGATGGGTGCTCTGCTGGACCCTGGTCAATCTTTTCCGAAGCCATTCAGTCCTCAATCTCCT
  GTTCCTTGGCTATCCGTTTGGTGTTTATGTTCCTCTCTACTGTTTCCACCAAGATAGCAGAGCCCATCTT
  CTTCTCACAGACTATGTGGTTCAGCACCAGGCAGTAGAGGAGGCGGCTTCAAATGTGGGCAGTTTGGCCA
  GATCCAAAGATTTCCTCTCCTTACTGCTGGAGTCTCTAAAAGAACAGTTTAATAATGCCACGCCCATCCC
  CACCCACAGCTGCCCCCTATCTCCTGATCTCATTCGCAATGAAGTAGAATGTCTGAAAGCAGATTTCAAC
  CACAGAATCAAGGAAGTTCTCTTCAACTCCCTCTTCAGTGCCTACTACGTTGCATTTCTCCCGCTGTGTT
  TTGTGAAGAGTACCCAGTATTACGACATGCGCTGGTCGTGTGAGCACCTCATTATGGTGTGGATCAATGC
  TTTTGTCATGCTCACCACGCAACTGCTGCCATCCAAATACTGTGACTTGCTACACAAATCAGCTGCTCAC
  CTGGGCAAGTGGCAGAAGTTGGAGCATGGGTTCTACAGCAATGCTCCACAGCACATTTGGTCAGAAAACA
  CAATATGGCCTCAAGGGGTGTTGGTGCGGCACAGCAGATGTTTATATCGAGCCATGGGACCTTACAATGT
  AGCAGTGCCTTCAGATGTATCCCATGCCCGCTTTTATTTCCTGTTTCACCGCCCATTACGAGTGCTGAAC
  CTGCTTATCCTCATTGAAGGCAGTGTTGTCTTCTACCAGCTGTATTCCTTGCTGCGGTCGGAGAAGTGGA
  ACCACACGCTCTCCATGGCCCTCATCCTTTTCTGCAACTACTATGTTTTATTTAAGCTCCTCCGGGACAG
  AATAGTCTTAGGCAGGGCATACTCCTACCCCCTCAACAGTTACGAACTCAAGGCCAACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205286
• Insert Size: 1461 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205286.1, NP_001192215.1
• RefSeq Size: 2617 bp
• RefSeq ORF: 1461 bp
• Locus ID: 67846
• UniProt ID: Q9CYC3
• Cytogenetics: 16 B4
• Gene Summary: Regulates autophagy by controlling the spatial distribution and levels of the intracellular phosphatidylinositol 4-phosphate (PtdIns(4)P) pools (By similarity). Modulates (PtdIns(4)P) levels by regulating the ER-to-Golgi trafficking of the phosphatidylinositide phosphatase SACM1L (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) has an alternate 5' UTR exon, compared to variant 1. Variants 1 and 2 encode the same isoform (1).