Tbx1 (NM_001285472) Mouse Untagged Clone

CAT#: MC227909

Tbx1 (untagged) - Mouse T-box 1 (Tbx1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tbx1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227909 representing NM_001285472
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTTCAGCACAGTCACCAGGGACATGGAAGCCTTCGCAGCCAGCAGTCTGAGCGGCCTGGGATCCC
  CGTCGCCTGGCGCCGACCCGTTCGGCCCTCGCGAGCCGCCGCCACCGCGCTACGATCCGTGCGCTGCAGT
  CCCCGGTGCCCCGGGCCCGCCGCCGCCGCGCGCCTATCCTTTCGCGCCCGCCCCCGGGGCGGCTGGCAGC
  TCGGCGGCGGAGTCCGAGGGTCCGGGGGCTAGCCGCGCGGCTGCGGTCAAGGCTCCGGTGAAGAAGAACC
  CGAAGGTGGCCAGCGTGAGCGTGCAGCTGGAGATGAAGGCGCTGTGGGACGAGTTCAATCAGCTGGGCAC
  CGAGATGATCGTCACCAAGGCAGGCAGACGAATGTTCCCCACGTTCCAAGTGAAGCTTTTTGGAATGGAT
  CCCATGGCCGACTACATGCTGCTCATGGACTTTGTGCCCGTAGATGACAAGCGCTACCGGTATGCTTTCC
  ATAGCTCCTCCTGGCTGGTGGCCGGCAAGGCAGATCCTGCTACACCTGGCCGAGTACACTACCACCCGGA
  CTCGCCGGCTAAGGGCGCACAGTGGATGAAACAGATTGTGTCTTTCGACAAGCTGAAACTGACCAATAAC
  CTGCTGGATGACAATGGCCATATTATTCTCAACTCCATGCACAGATATCAGCCCCGATTCCATGTTGTCT
  ATGTGGACCCTCGAAAAGACAGTGAGAAATATGCAGAGGAGAACTTCAAAACTTTTGTGTTTGAGGAGAC
  ACGCTTCACTGCAGTCACTGCCTACCAGAATCACCGGATCACGCAGCTTAAGATTGCCAGCAACCCCTTC
  GCCAAAGGCTTCCGGGATTGCGACCCGGAGGACTGGCCCCGGAACCACCGGCCCGGAGCGCTGCCGCTCG
  TGAGTGCCTTTGCTCGCTCTCGGAATCCCGTGGCTTCCCCCACGCAGCCCAATGGCTCAGACAAAGACGC
  TGCAGAAGCCCGGCGCGAGTTCGACCGTGACTCCGGACCCGCAGCGCTCGGCGACGCTACGCACCCGCCG
  CAGCTGCTGGCGCGCGTGCTGAGCCCCGCACTGCCCGGGCCTGGCGGCCTCGTCCCGCTACCCGGCGGAT
  CCGGAGGCCGCCACAGTCCCCCGCACGCCGATCTGCGCCTGGAGGCGCCGGGCGCGTCCGAGCCGCTGCA
  CCACCATCCCTACAAGTACCCGGCCGCCGCCTACGACCACTACCTCGGGGCCAAGAGCCGGCCGGCGCCC
  TACCCGCTGCCAGGCCTGCGCGGCCACGGCTACCACCCGCACGCGCACCCGCACGCGCACCCGCACCATC
  ACCACCACCCCGCGGTGAACCCGGCCGCCGCCGCCGCTGCTGCCGCAGCAGCCAACGTGTACTCGTCGGC
  GGCCGCGCCGCCCGGTGCCTACGACTACTGCCCCAGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285472
• Insert Size: 1440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285472.1, NP_001272401.1
• RefSeq Size: 1858 bp
• RefSeq ORF: 1440 bp
• Locus ID: 21380
• Cytogenetics: 16
• Gene Summary: Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.