Brap (NM_001289543) Mouse Untagged Clone

CAT#: MC227727

Brap (untagged) - Mouse BRCA1 associated protein (Brap), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Brap
• Synonyms: 3010002G07Rik; BRAP2; IMP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227727 representing NM_001289543
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTCCCTAAAAGAAGACGTTCGGCGCAGTGCCATGCTGTGTGTCCTCACCGTCCCTGCCACCATGA
  CCAGTCATGACCTTATGAAGTTTGTTGCCCCATTCAATGATGTAATTGAACAAATGAAAATCATCAGGGA
  CTCTACTCCGAATCAGTACATGGTACTGATCAAGTTCAGTGCGCAGGCTGATGCAGACAGTTTCTACATG
  GCGTGCAATGGCCGCCAGTTCAACTCAATCGAAGATGACGTCTGCCAGCTGGTCTATGTGGAAAGGGCTG
  AAGTGCTGAAATCTGAAGATGGCGCCAGCCTCCCCGTGATGGACCTGACGGAGCTGCCCAAGTGCACTGT
  GTGTCTGGAGCGGATGGACGAGTCTGTGAATGGCATCCTCACCACCCTCTGCAACCACAGCTTCCATAGT
  CAGTGTCTGCAGCGGTGGGATGACACCACGTGTCCTGTGTGCCGATACTGTCAAACGCCAGAGCCAGTGG
  AAGAAAACAAATGTTTTGAGTGTGGTGTCCAGGAAAACCTCTGGATTTGTTTAATATGCGGCCACATAGG
  CTGTGGGCGGTACGTGAGTCGGCATGCTTACAAGCACTTTGAGGAGACCCAGCACACATACGCCATGCAG
  CTCACCAACCATCGAGTCTGGGACTATGCTGGAGATAATTATGTCCATCGACTGGTTGCAAGCAAGACGG
  ATGGAAAGATCGTTCAGTACGAGTGTGAGGGCGACACCTGCCAGGAAGAGAAGATAGATGCCTTACAGTT
  AGAGTACTCGTACCTGTTGACAAGCCAGCTGGAATCGCAGCGGATATACTGGGAGAACAAAATCGTGCGC
  ATAGAGAAGGACACGGCAGAGGAGATTAACAACATGAAGACCAAGTTTAAAGAGACCATCGAGAAGTGTG
  ACAGCCTGGAGCTCAGGCTCAGTGACCTCCTGAAGGAGAAGCAGTCTGTGGAAAGGAAGTGTACCCAGCT
  GAACACCAGAGTGGCCAAGCTCAGCACGGAGCTGCAGGAGGAGCAGGAGCTGAACAAGTGTCTGCGCGCC
  AACCAGCTGGTGCTGCAGAACCAGCTCAAGGAGGAGGAGAAGCTGCTGAAGGAGACCTGTGCCCAGAAAG
  ACCTGCAGATCACCGAGATCCAGGAGCAGCTGCGCGATGTCATGTTCTACCTGGAGACACAGCAGCAGAT
  CAGCCACCTGCCTGCGGAGACGAGGCAGGAGATCCAGGAAGGCCAGATCAACATCGCCATGGCCTCAGCG
  CCCAACCCACCCTCTTCCGGGGCCGGTGGGAAGCTGCAGTCCAGAAAGGGCCGCAGCAAGAGGGGCAAGT
  GA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289543
• Insert Size: 1332 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289543.1, NP_001276472.1
• RefSeq Size: 3541 bp
• RefSeq ORF: 1332 bp
• Locus ID: 72399
• Cytogenetics: 5 F
• Gene Summary: Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon in the 5' coding region and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.