Kynu (NM_001289593) Mouse Untagged Clone

CAT#: MC227647

Kynu (untagged) - Mouse kynureninase (L-kynurenine hydrolase) (Kynu), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kynu
• Synonyms: 4432411A05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227647 representing NM_001289593
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAGCCTTCGCCTCTTGAGCTTCCAGTTGATGCAGTGCGGCGCATCGCGGCTGAACTCAATTGTG
  ACCCAACAGATGAGAGGGTTGCTCTCCGCTTGGATGAGGAAGATAAACTGAGTCATTTTAGGAACTGTTT
  TTATATTCCCAAAATGCGGGACCTGCCTTCAATTGATCTATCTTTAGTGAGTGAGGATGATGATGCCATC
  TATTTCCTGGGAAATTCCCTTGGCCTTCAACCGAAAATGGTTAGGACATACCTGGAGGAAGAACTAGATA
  AGTGGGCCAAGATGGGAGCCTATGGCCATGATGTAGGCAAACGCCCTTGGATTGTAGGGGATGAGAGTAT
  TGTAAGCCTTATGAAGGACATTGTAGGAGCCCATGAGAAAGAAATAGCTCTAATGAATGCTTTGACTATT
  AATTTACATCTCCTGCTGTTATCATTCTTTAAGCCTACTCCAAAGCGGCACAAAATTCTTCTAGAAGCCA
  AAGCCTTCCCTTCTGATCATTATGCTATTGAGTCACAGATTCAACTTCACGGACTTGATGTTGAGAAAAG
  TATGCGGATGGTAAAGCCACGAGAGGGGGAAGAGACCTTAAGGATGGAGGACATACTGGAAGTAATCGAG
  GAGGAAGGAGACTCGATCGCCGTGATCCTGTTCAGTGGGCTGCACTTTTATACTGGACAGCTGTTCAACA
  TTCCTGCCATAACAAAAGCTGGACATGCAAAGGGCTGTTTTGTTGGCTTTGACCTAGCACATGCAGTTGG
  AAATGTTGAACTCCGCTTACATGACTGGGGTGTTGACTTTGCCTGCTGGTGTTCCTATAAGTATTTAAAT
  TCAGGAGCTGGAGGTCTGGCTGGTGCCTTTGTCCACGAGAAACATGCTCATACTGTCAAGCCTGCGTTAG
  TGGGATGGTTCGGCCATGACCTCAGTACAAGGTTTAACATGGATAACAAACTACAATTAATCCCCGGGGC
  CAATGGATTCCGAATTTCAAACCCTCCCATTTTGTTGGTCTGCTCCTTGCACGCCAGTTTAGAGGTCTTT
  CAGCAAGCAACTATGACTGCGCTGAGAAGAAAATCCATTCTGCTGACAGGTTATCTGGAATACATGCTCA
  AACATTACCACAGCAAAGATAACACCGAAAACAAGGGGCCGATTGTGAATATCATCACCCCGTCCAGAGC
  AGAGGAGCGTGGCTGCCAGTTAACACTCACCTTTTCCATTCCCAAGAAAAGCGTTTTTAAGGAACTAGAA
  AAAAGAGGAGTCGTTGTATGTATATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289593
• Insert Size: 1287 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289593.1, NP_001276522.1
• RefSeq Size: 1604 bp
• RefSeq ORF: 1287 bp
• Locus ID: 70789
• UniProt ID: Q9CXF0
• Cytogenetics: 2 B
• Gene Summary: Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains a 3' terminal exon which extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.