Jmjd4 (NM_001205068) Mouse Untagged Clone

CAT#: MC227580

Jmjd4 (untagged) - Mouse jumonji domain containing 4 (Jmjd4), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Jmjd4
• Synonyms: 6430559I23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227580 representing NM_001205068
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCGGGAGACACGCACCTTTGCCGAGCGGTACTACCGGGACCTCCGAGACCCAGTCCCTAGCGGCG
  GTGGCGGCCCCACACCCAGCGGCGTGACCTTCATTCAGACCCCGAACGCCTTCTCCTATGCTGACTTCGT
  GAAGGGCTTCCTGCTGCCTAACCTGCCCTGCGTGTTTTCTAGCGCCTTCACCGAGGGCTGGGGCAGCAGG
  CGGCGCTGGGTGACCTCGGAGGGAAAGCCTGACTTCGAGTATCTGCAGCAGAAGTATGGAGACGCGGTTG
  TACCGGTTGCCAACTGTGGAGTGCGAGAATACAATTCAAACCCCAAGGAGCACATGTCCTTCCGCGACTA
  TATCAGTTACTGGAAGGACTACATCCAAGGGAGTTACTCCTCTTCGCGGGGCTGCCTCTACCTCAAGGAC
  TGGCATCTGTGCAGAGACTCCTTGGTGAATGACTTGGAAGACATATTCACCCTGCCCGTGTACTTCTCGT
  CAGACTGGCTGAATGAGTTCTGGGACGTCCTCAATGTGGATGACTATCGTTTTGTCTATGCAGGCCCCAG
  GGGCACCTGCTGGTCAGTCAACATCTGTGGGAAGAAGAAATGGTTGTTCTTCCCGCCAGGCGAGGAGGAA
  GCCCTCCGGGACTGCCATGGTAACCTGCCCTATGATGTGACTTCCACTGAGCTCCTGGACACCCACCTGT
  ATCCCAAGATCCAGCACCACAGCCTTCCCATTGAAGTCATACAGGAGCCTGGTGAGATGGTGTTTGTGCC
  CAGCGGGTGGCACCATCAAGTGTACAACCTGGATGACACCATTTCTATTAACCACAACTGGGTCAATGGC
  TGCAACCTGCCAAACATGTGGCACTTCCTGCAGCAAGAGCTCCAGGCTGTGCAGCATGAGGTCGAAGAAT
  GGAAGGACTCCATGCCTGACTGGCACCACCACTGCCAGGTCATCATGAAGTCCTGCACAGGGATCAATTT
  TGAAGAATTTTACCATTTCCTCAAAGTCATCGCCGAGAAGAGGCTCCTTGTCCTGGAGCAAGGACTGAAG
  GGGGATTCAGGGGACAGCCGGAGCCTAGACCTGGGCCTACAGCAGGCTGCATTTGATATTGGCCGCCTTG
  CAGATGTGCTGGCCTCTGTGGTTGTGAATCCTGACTTTCAGAGAGTGGACACCAGCGCATTCTCACCACA
  GCCAGAAGAGTTGCTTCAGCAGCTGGAGGACGCAGTGGCTGCTGCAGAGGCCCTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001205068
• Insert Size: 1248 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205068.1, NP_001191997.1
• RefSeq Size: 4409 bp
• RefSeq ORF: 1248 bp
• Locus ID: 194952
• UniProt ID: Q8BFT6
• Cytogenetics: 11 B1.3
• Gene Summary: Catalyzes the 2-oxoglutarate and iron-dependent C4-lysyl hydroxylation of ETF1 at 'Lys-63' thereby promoting the translational termination efficiency of ETF1 (By similarity). Not essential for embryonic stem cell (ESC) maintenance and the embryonic and postnatal development (PubMed:27147518).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.