Mknk1 (NM_001285488) Mouse Untagged Clone

CAT#: MC227577

Mknk1 (untagged) - Mouse MAP kinase-interacting serine/threonine kinase 1 (Mknk1), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mknk1
• Synonyms: 2410048M24Rik; Mnk; Mnk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227577 representing NM_001285488
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGCAGTGAGCCCCTTCCCATCGTGGATTCTGACAAGAGGAGGAAGAAGAAGCGTAAGACCCGGG
  CCACCGACTCTCTGCCAGGAAAGTTTGAAGATGTGTACCAGCTGACCTCGGAATTGCTGGGAGAAGGAGC
  CTATGCCAAAGTCCAGGGTGCTGTGAACCTACAGAGTGGGAAGGAGTATGCTGTCAAAATCATCGAGAAG
  CAAGCCGGGCACAGTCGAAGTCGAGTGTTCCGTGAGGTGGAGACACTGTATCAGTGTCAAGGGAACAGGA
  ACATTTTGGAGCTGATTGAATTCTTTGAAGATGACACACGGTTTTACTTGGTCTTTGAGAAATTGCAAGG
  AGGCTCCATCCTAGCACACATCCAGAAGCGGAAGCACTTCAATGAGCGAGAAGCCAGTAGAGTGGTGCGG
  GACGTCGCCACTGCCCTTGACTTCCTGCACACTAAAGGCATTGCTCACCGTGATCTGAAGCCAGAAAACA
  TACTGTGTGAATCTCCAGAAAAGGTGTCTCCGGTGAAAATTTGTGACTTTGACTTGGGCAGTGGGGTAAA
  GTTGAACAACTCCTGCACTCCCATAACCACGCCAGAGCTGACTACTCCATGCGGCTCTGCAGAGTATATG
  GCACCTGAGGTGGTGGAGGTCTTTAGGGACGAGGCTACTTTCTATGACAAGCGCTGTGACCTGTGGAGCC
  TGGGTGTGGTCCTCTACATCATGCTGAGTGGCTACCCCCCCTTTGTAGGTCACTGTGGGGCTGACTGTGG
  CTGGGACCGGGGAGAGGTATGCAGGATGTGCCAGAACAAGCTGTTTGAGAGCATCCAGGAAGGCAAATAC
  GAGTTTCCTGACAAAGACTGGGCTCACATCTCCAACGAGGCCAAAGACCTCATCTCTAAGCTCCTGGTTC
  GAGATGCGAAGCAAAGACTCAGTGCTGCCCAGGTTCTGCAGCATCCATGGGTACAAGGGCAAGCTCCAGA
  AAGGGGACTCCCCACGCCGCAAGTCCTTCAGAGAAACAGCAGCACCATGGACTTGACTCTCTTCGCAGCT
  GAGGCCATTGCCCTGAACCGCCAGCTGTCTCAGCATGAGGAGAATGAACTGGCCGAGGAACAGGAGGCCC
  TAGCTGAGGGCCTCTGCTCCATGAAGCTGTCCCCTCCATCCAAATCTCGCCTGGCTCGAAGGCGAGCCCT
  GGCCCAGGCTGGCCGAAGCCGAGATGCAAACCCATGTTTGACACCGGCAGGGCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285488
• Insert Size: 1248 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285488.1, NP_001272417.1
• RefSeq Size: 2598 bp
• RefSeq ORF: 1248 bp
• Locus ID: 17346
• UniProt ID: O08605
• Cytogenetics: 4 D1
• Gene Summary: This gene encodes a serine-threonine protein kinase that is activated by extracellular signal-regulated kinase or p38 mitogen-activated protein kinases, and it may function in cytokine and environmental stress responses. This kinase is required for phosphorylation of eukaryotic translation initiation factor 4E but it is not required for cell growth during development. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Oct 2013]
  Transcript Variant: This variant (2) lacks a portion of the 5' UTR and 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a. Both variants 2 and 3 encode isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.