Agxt (NM_001276710) Mouse Untagged Clone

CAT#: MC227455

Agxt (untagged) - Mouse alanine-glyoxylate aminotransferase (Agxt), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Agxt
• Synonyms: AGT; Agt1; Agxt1; SPT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227455 representing NM_001276710
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCGTATCAGCTGCTGGTGCCACCGCCAGAGGCCCTGAGCAAGCCCCTGTCAGTTCCTACGAGGC
  TCCTGTTGGGTCCGGGGCCCTCCAACCTGGCTCCCCGTGTGCTGGCGGCGGGAAGTCTGAGAATGATTGG
  CCACATGCAAAAAGAGATGCTTCAGATCATGGAGGAGATCAAGCAAGGCATCCAGTATGTGTTCCAGACC
  AGGAACCCCCTCACACTGGTTGTCAGCGGTTCAGGGCACTGCGCCATGGAGACTGCCCTGTTCAACCTCC
  TAGAGCCTGGGGACTCCTTTCTAACCGGAACCAATGGCATCTGGGGAATGCGGGCTGCAGAGATTGCTGA
  TCGCATTGGAGCCCGTGTGCACCAGATGATCAAGAAGCCTGGAGAACATTATACACTGCAGGAGGTGGAG
  GAGGGCCTGGCCCAACACAAACCGGTATTGCTGTTCCTGGTCCACGGGGAGTCATCCACTGGCGTGGTGC
  AGCCCCTGGATGGTTTTGGGGAGCTCTGCCATAGGTACCAGTGCCTACTCCTGGTGGACTCAGTGGCATC
  CCTGGGCGGGGTTCCTATCTACATGGACCAACAAGGCATTGACATCATGTACTCTAGCTCTCAGAAGGTC
  CTGAATGCCCCACCAGGGATCTCCCTCATCTCCTTCAACGACAAGGCCAAATACAAGGTCTACTCCCGGA
  AGACAAAGCCAGTCTCCTTCTACACAGACATCACATACTTGGCCAAATTGTGGGGATGTGAGGGCGAGAC
  CAGAGTAATTCATCATACCACACCTGTCACCAGCTTATACTGCCTGAGGGAGAGCCTGGCACTCATTGCA
  GAGCAGGGCCTGGAGAATTGCTGGCGGCGTCACAGGGAGGCTACAGCACATCTGCACAAACACCTGCAGG
  AGATGGGCTTAAAGTTCTTTGTGAAGGATCCGGAAATCCGGCTACCCACAATCACCACCGTGACTGTGCC
  TGCCGGCTACAACTGGAGGGACATCGTCAGCTATGTGCTGGACCACTTCAGCATTGAAATCTCTGGGGGT
  CTTGGGCCCACTGAGGAGAGGGTGCTACGGATTGGCCTCCTGGGCTACAACGCCACCACTGAGAATGTGG
  ACCGTGTGGCCGAGGCCCTGAGGGAGGCCCTGCAGCATTGTCCTAAGAACAAGTTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276710
• Insert Size: 1179 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276710.1, NP_001263639.1
• RefSeq Size: 1553 bp
• RefSeq ORF: 1179 bp
• Locus ID: 11611
• Cytogenetics: 1 47.0 cM
• Gene Summary: Dual metabolic roles of gluconeogenesis (in the mitochondria) and glyoxylate detoxification (in the peroxisomes).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) includes an upstream AUG start codon (uAUG) and a downstream AUG start codon (dAUG). Isoform 2 is encoded from the CDS with the dAUG start codon, and is a peroxisomal enzyme. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.