Skp2 (NM_001285980) Mouse Untagged Clone

CAT#: MC227443

Skp2 (untagged) - Mouse S-phase kinase-associated protein 2 (p45) (Skp2), transcript variant 3

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Skp2
• Synonyms: 4930500A04Rik; FBXL1; FWD1; p45
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227443 representing NM_001285980
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGTCTCGGCCTTGGAGAAGGAGGAGGTGGACAGTGAGAACATCCCACATGGACTGCTCTCAAACC
  TCGGCCACCCCCAGAGCCCTCCAAGGAAACGAGTCAAGGGCAAAGGGAGTGACAAAGACTTTGTGATCAT
  CCGTCGGCCGAAGCTTAGTCGGGAGAACTTTCCAGGTGTCTCCTGGGACTCCCTTCCAGATGAGCTGCTC
  CTTGGGATCTTTTCCTGTCTGTGCCTCCCTGAGCTGCTGAGAGTCTCGGGCGTTTGCAAGAGGTGGTACC
  GCCTCTCGCTCGATGAGTCTCTCTGGCAGTCCCTCGACCTCGCGGGTAAAAATCTGCACCCAGACGTGAC
  TGTGCGCTTGCTCTCCCGCGGGGTGGTCGCCTTCCGCTGCCCTCGGTCCTTTATGGAGCAGCCGCTGGGT
  GAAAGCTTCAGCTCTTTCCGGGTACAGCACATGGACCTGTCGAACTCAGTGATAAATGTGTCGAACCTCC
  ATAAGATTCTGTCCGAGTGCTCCAAGCTGCAGAATCTAAGCCTGGAAGGCCTGCAGCTCTCAGACCCCAT
  TGTCAAGACTCTTGCACAGAATGAAAACTTGGTGCGACTAAACCTTTGTGGGTGCTCTGGGTTTTCTGAA
  TCTGCCGTGGCGACTCTGCTAAGCAGCTGCTCCAGACTGGATGAGCTAAATCTCTCCTGGTGCTTTGACT
  TCACTGAAAAGCACGTGCAAGCGGCTGTGGCACATTTACCAAACACCATCACCCAGCTGAACCTCAGCGG
  CTACCGAAAGAACCTCCAGAAAACAGATCTTTGTACCATAATTAAACGATGCCCCAACCTCATCCGCCTC
  GACTTAAGTGACAGTATCATGCTAAAGAATGACTGCTTTCCAGAATTTTTTCAACTCAACTACCTCCAAC
  ACCTCTCGCTCAGCCGGTGCTATGATATAATACCTGATACTCTACTTGAACTTGGAGAAATTCCTACACT
  AAAAACGCTACAAGTTTTTGGAATCGTGCCAGAGGGAACCCTTCAGCTACTGAGGGAAGCTCTTCCTCGG
  CTGCAGATTAACTGCGCCTATTTCACCACCATTGCAAGGCCAACTATGGACAGCAAGAAGAACCTGGAGA
  TTTGGGGTATCAAGTGCCGACTGACTCTGCAAAAGCCCAGTTGTCTATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001285980
• Insert Size: 1170 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285980.1, NP_001272909.1
• RefSeq Size: 3140 bp
• RefSeq ORF: 1170 bp
• Locus ID: 27401
• Cytogenetics: 15 A1
• Gene Summary: Substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. The SCF complex provides substrate specificity and interacts with both, the E2 ubiquitin-conjugating enzyme and the substrate. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, lacks part of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (c) has a shorter N-terminus, compared to isoform a.