Lhx2 (NM_001290646) Mouse Untagged Clone

CAT#: MC227299

Lhx2 (untagged) - Mouse LIM homeobox protein 2 (Lhx2), transcript variant 2

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lhx2
• Synonyms: ap; apterous; Lh-2; LH2A; Lim2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227299 representing NM_001290646
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGTCCATCAGCAGTGACCGGGCAGCGTTGTGTGCTGGCTGTGGGGGCAAGATCTCTGACCGCTACT
  ACCTGCTGGCAGTAGACAAGCAATGGCACATGCGCTGCCTCAAGTGCTGTGAATGCAAGCTCAACCTGGA
  GTCGGAACTCACCTGCTTCAGCAAGGATGGCAGCATCTACTGCAAAGAAGACTACTACAGGCGGTTCTCT
  GTGCAGCGCTGCGCCCGCTGCCACCTGGGCATCTCGGCCTCAGAGATGGTGATGCGCGCTCGGGACTTGG
  TTTATCACCTCAACTGCTTCACATGCACAACGTGTAACAAGATGCTGACGACCGGCGACCATTTCGGCAT
  GAAGGACAGCCTGGTCTATTGCCGCTTGCACTTCGAGGCTCTGCTGCAGGGCGAATACCCAGCACACTTT
  AACCATGCCGACGTGGCAGCGGCGGCAGCCGCAGCCGCAGCAGCTAAGAGTGCAGGATTGGGCTCAGCCG
  GGGCTAATCCGCTGGGTCTTCCCTACTACAACGGCGTGGGCACTGTGCAAAAGGGGAGGCCGAGAAAGCG
  CAAGAGTCCAGGACCCGGGGCAGATCTGGCAGCTTACAACGCCGCGCTTAGCTGTAACGAGAACGATGCT
  GAACACCTGGATCGTGACCAGCCCTACCCCAGCAGCCAAAAGACAAAGCGCATGCGCACCTCCTTCAAGC
  ACCACCAGCTTCGGACAATGAAGTCTTACTTTGCCATTAACCACAATCCCGATGCCAAGGACTTGAAGCA
  GCTTGCGCAAAAGACCGGCCTCACCAAGAGAGTCCTCCAGGTCTGGTTTCAGAATGCCCGGGCCAAGTTC
  AGGCGCAACCTTTTACGGCAGGAAAACACGGGCGTGGACAAGACGTCAGATGCCACGCTGCAGACAGGGA
  CGCCGTCAGGGCCCGCCTCGGAGCTGTCCAACGCCTCGCTCAGCCCCTCCAGCACGCCTACAACCCTCAC
  AGACTTGACTAGCCCCACCCTGCCGACTGTGACGTCAGTCTTAACTTCTGTGCCTGGCAACCTGGAGGGC
  CACGAGCCCCACAGCCCTTCACAAACGACTCTTACCAACCTTTTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290646
• Insert Size: 1098 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290646.1, NP_001277575.1
• RefSeq Size: 2234 bp
• RefSeq ORF: 1098 bp
• Locus ID: 16870
• Cytogenetics: 2 B
• Gene Summary: Acts as a transcriptional activator. Stimulates the promoter of the alpha-glycoprotein gene. Transcriptional regulatory protein involved in the control of cell differentiation in developing lymphoid and neural cell types.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate 5' terminal exon and it thus differs in the 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a.