Ascc1 (NM_001199187) Mouse Untagged Clone

CAT#: MC227246

Ascc1 (untagged) - Mouse activating signal cointegrator 1 complex subunit 1 (Ascc1), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ascc1
• Synonyms: 1810015P09Rik; AI550520; ASC1p50; CGI-18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC227246 representing NM_001199187
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTTCTACGCCCACAGATTGTAACGTTCGATGGACGGAATTATAGAAAGAATCCCATCCAGGAGA
  AACAGTACCAACATGAAGAAGACGAGGATTTCTATCCTGACTCCATGGAATACTCTGATGAGCCCTGCGG
  TGCCTACGAGGTGGCACAGACCCCTCACGGATTCCGGGCCACCGTCAGTGCCCCTAGCCTGCTCTACAAG
  CACATAGTTGGAAAGAGAGGGGATACCAAGAAAAAGATAGAAGTGGAGACTAAAACATCTATTAACATTC
  CGAAGCATGGGCATGAAGGGGAAATCGTGATCACTGGTCAGCATCGAAATGGCGTGGTCTCAGCGCGAAC
  ACGGATCGACGTGCTCCTGGACACGTTCAGGAGGAGGCAGCCTTTTACCCACTTCCTTTCCTTCTTCCTC
  AATGAAGTTGAGGTTCAGGAACGGTTTCTGATGTTCCAGGAGGAGGTGCTGAGGAAGTGCTCCAAGGATC
  GTGGAGTTGATAGCACCATTTTTCAGAACCCCAAAAAGCTTCACCTAACTATTGGGATGTTGGTACTTCT
  AAGTGAGCAGGAGATCCAGCAGACGTGTGAGATCCTGCAGCGCTGTAAAGAGGAATTCATTAATGACATT
  TCTGGAGGCAGACCCCTGGAAGTCGAGATGGCAGGCATCGAATACATGAATGACGATCCTGCTATGGTGG
  ATGTTCTTTATGCCAAAGTCCACATGAAAGATGGCTCCAACAGGCTGCAGGAGCTGGTTGATCGAGTGCT
  GGAGCGTTTTCAGTCCCTGGGACTCATCGTGAAAGAGTGGACCAGTGTGAAACTGCACGCCACGGTCATG
  AACACACTGCTCAGGAAAGACCCCAACGCGGAAGGCAGGTACAATCTCTACACAGCAGATGGCAAATATA
  TCTTCAAGGAGAGAGAGTCTTTTGATGGCCGGAACATTTTAAAGACATTTGAGAACTTCTACTTTGGTTC
  CCTGAGGCTCAATTCTATTCACATCTCCCAGAGGTTCACGGTGGACAGCTTTGGAAACTATGCCTCCTGC
  GGACATGTTGACTTCTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001199187
• Insert Size: 1071 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199187.2, NP_001186116.1
• RefSeq Size: 1492 bp
• RefSeq ORF: 1071 bp
• Locus ID: 69090
• UniProt ID: Q9D8Z1
• Cytogenetics: 10 B4
• Gene Summary: Plays a role in DNA damage repair as component of the ASCC complex. Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation. In cells responding to gastrin-activated paracrine signals, it is involved in the induction of SERPINB2 expression by gastrin. May also play a role in the development of neuromuscular junction.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.